酵素

In this study, we isolate, purify and characterize endo-β-glucanase by Ascomycete Xylaria regalis. The cellulose-containing medium is used to induce the production of the cellulose-degrading enzymes. When these enzymes are produced at maximum level, we harvest and filtrate the culture medium. The filtrate is purified by anion exchanger column and hydrophobic interaction column (phenyl-650M). The purification fold of endo-β- glucanase increases 4.3 times, the specific activity approaches 3.7 unit/mg, and the recovery is 27.6%, respectively.

为了分离且纯化此株菌产生在培养基中的endo-β-glucanase，先将此菌在cellulose液态培养基中大量培养，在此酵素达到最高峰时，利用滤纸过滤将菌丝及菌液分开，收集粗酵素液，再经过阴离子交换树脂及疏水性层析管柱(Phenyl-650M)等步骤予以纯化，所得到最终酵素纯度为 4.3倍，比活性为3.7 unit/mg，回收率为27.6%。

Intestinal juice contains all food enzymes (carbohydrase, protease and lipase) for complete digestion of food.

小肠液含有所有食物酵素(碳水化合物酵素、蛋白质酵素和脂肪酵素)，可完成食物的消化。

Contain enzymes (carbohydrase, protease and lipase) which digest carbohydrates, proteins and fats.

含有可消化碳水化合物，蛋白质和脂脂的酵素碳水化合物酵素，蛋白质酵素和

In clinical. acetylcholinesterase inhibitors reduce the breakdown of synaptic acetylcholine, have been modestly effective in improving the cognitive deficits of Alzheimer's disease.The goal of this work is to determine enzyme kinetics and mechanisms of acetylcholinesterase and butyrlcholinesterase inhibition by five cardiovascular drugs, lovastatin, simvastatin, amlodipine besylate, nifedipine, and hydralazine hydrochloride, and two benzodiazepines, diazepam and chlordiazepoxide hydrochloride. All drugs in this study are reversible mixed-type inhibitors of acetylcholinesterase and butyrylcholinesterase. The pKi values for acetylcholinesterase and butyrylcholinesterase inhibition by the cardiovascular drugs are linearly correlated with the molecular weights of the drugs with the slopes of 0.005 and 0.0021, respectively. Therefore, van der Waals' interactions between acetylcholinesterase and the cardiovascular drugs are stronger than those between butyrylcholinesterase and the drugs. This is probably due to a smaller active site gorge and a more significant peripheral anionic substrate binding site of acetylcholinesterase than those of butyrylcholinesterase.

本研究之目的系决定乙醯胆碱酯酵素和丁醯胆碱酯酵素被五种心脏血管药物lovastatin， simvastatin， amlodipine besylate， nifedipine、hydralazine hydrochloride和两种benzodiazepines：diazepam和Chlordiazepoxide hydrochloride的抑制作用之动力学及机转，这些药物都是乙醯胆碱酯酵素和丁醯胆碱酯酵素的可逆性、混合型之抑制剂，实验结果显示，五种心血管药物对於乙醯胆碱酯酵素及丁醯胆碱酯酵素抑制之pKi值和药物之分子量呈直线之关系，斜率分别为0.005及0.0021因此丁醯胆碱酯酵素，与心血管药物间之凡德瓦作用（van der Waals' ）比乙醯胆碱酯酵素与心血管药物间者弱，这可能原因是丁醯胆碱酯酵素之活性区域比乙醯胆碱酯酵素之活性区域更宽广，但丁醯胆碱酯酵素之周边阴离子区域不及乙醯胆碱酯酵素之周边阴离子区域者明显重要，由於五种心血管药物对於乙醯胆碱酯酵素和丁醯胆碱酯酵素抑制之pKi值存在著线性关系表示此种抑制作用系经过共同之反应机理。

Bamboo mosaic virus encodes a 155-kDa replicase that the middle region contains a helicase-like domain according to the existence of several conserved featured motifs of helicase. Previous studies showed that the helicase-like domain exhibits both ATPase and RNA 5'-triphosphotase activities, and Mg2+ is essential for both activities. Mutagenesis of conserved motifs showed these amino acids play important roles for enzymatic activities. These results suggested that the properties of this denatured and renatured protein are coincident with other SF1 helicases.

从胺基酸序列分析显示，竹嵌纹病毒复制酵素中间区域的类解螺旋酵素具有解螺旋酵素的保留性胺基酸区域，经实验显示此蛋白具有ATPase和RNA 5'-triphosphatase活性，而且是依赖镁离子进行反应的，其中保留性胺基酸的突变会破坏其催化能力，说明这在大肠杆菌表达的蛋白质经过变性及重新摺叠的过程，具有和其他SF 1解螺旋酵素相似的特性。

Collagenase activity was defined as the radioactivity extractable by 50% dioxane. Clostridial collagenase was used as a standard. The lower limit of this assay method was 0.01 units of clostridial collagenase. Among 17 glaucoma eyes, 6 had trace collagenase activity (around 0.01 units) in aqueous humor; and for cataract eyes, 8 out of 27 had trace collagenase acitivty.

我们所采用之测量方法可侦测出约0.01单位以上的胶原酵素；一般前房液之胶原酵含量非常低，许多患者无法以我们之方法利得胶原酵素之存在：17位青光眼患者中，只有6位可於其前房液中到得约0.01单往之胶原酵素；27位白内障急者中，有8位可测得微量胶原酵素；二者在统计学上与太大差异存在。

These ferments are made of the roofs, leaves and stems of 58 handpicked kinds of plant and vegetables through exclusive the biochemical technology, with the high-reactivity, high-stability and high-safety; The second is the fruit compound ferments(the approval No.J11000510836) with the abundant vitamin, minerals, favorable viable groups, multi-amino acid groups, oligosaccha groups and glucose , is made from handpicked 103 kinds of fruits and 20 kinds of Chinese herbal medicines through microbial treatment and fermentation method; The third is grain and bean high-fiber nutritious ferments (the approval No.J11000600369) with abundant proteases, amyloclast enzyme and lipolytic enzyme, is made from 60 kinds of grains and beans through special biologic technology, and is used to lose weight, expel toxin, clear the bowels, supply nutrition and activate cells in human body.

公司精选台湾58种植物、蔬菜的根、茎、叶天然原料，以独特的生化科技手段研制而成，不仅具有高活性、稳定性和安全性的特点，而且具有耐高温、耐强酸的特点，不含任何添加剂，其活性是当今世界任何厂家生产的酵素都不可比拟的。第二宝是水果复合酵素（检验审核号是J11000510836）。公司精选台湾103种水果，通过微生物科技处理，经半年多时间发酵酿制而成，含有丰富的维生素、矿物质、有益活菌群、氨基酸、果寡糖、葡萄糖等。第三宝是谷豆高纤营养酵素（检验审核号是J11000600369）。公司精选60种谷、豆及种子，用生物科技手段，经过特殊研制而成，含有丰富的蛋白质分解酵素、淀粉分解酵素及脂肪分解酵素。

In this study, we investigate the heat-induced inhibition of drug-metabolizing enzyme activity in mice fed with freeze-dried, heated freeze-dried or half amount autolyzed freeze-dried Brussels sprouts powder. 0.5% ethoxyquin diet was fed to another group of mice as a positive control. The study mice, four per group, were fed a diet consisting of 20% Brussels sprouts for 7 days. Each group had its own pair-fed group. We found significantly greater inductions of glutathione S- transferase, quinine reductase, and phase Ⅱ drug-metabolizing enzyme activities in groups fed within freeze-dried and heated freeze-dried Brussels sprouts than their pair-fed groups. These findings indicate that intestinal microflora still can hydrolyze glucosinolates without plant myrosinase activity, though the alteration of cytochrome- P-450 1A (CYP1A, a phase Ⅰ drug-metabolizing enzyme) induction was only 1.4-fold.

本实验欲进一步探討加热处理之抱子甘蓝对phase Ⅰ与phase Ⅱ解毒酵素活性之影响差异，实验分三组，抱子甘蓝(1)冷冻乾燥组（glucosinolate未裂解）(2)加热冷冻乾燥组(3)半量之裂解冷冻乾燥组（glucosinolate已裂解），(4)and 0.5% ethoxyquin diet was used as a positive control以2%重量混合成饲料，餵以小白鼠（4只／组）7天，结果发现，在第(2)组小白鼠GST phase Ⅱ酵素）活性显著上升，显示在植物裂解酵素不存在之状况下，肠胃道之微生物仍可促进裂解反应，但由CYP1A活性增加不显著（仅1.4倍）看来，根据定义：单独phase Ⅱ酵素活性增加，为monofunctional induction/inducer，可加速致癌物之代谢。

Cinnamomea, antrocamphin A was purified from previous ethanol extraction using bioactivity-guided fractionation. As results, the antrocamphin A could significantly inhibit NO and PGE2 autacoids production in LPS-induced RAW 264.7 macrophage cells. Meanwhile, the mRNA and protein expression levels of iNOS and COX-2 were inhibited by antrocamphin A in a dose-dependent manner. Antrocamphin A also reduced the translocation of NF-κB induced by LPS, which was associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 proteins level in the nucleus. This is the first report demostrating the A.

接著并以活性为导向的分离策略，从樟芝子实体乙醇抽出物中分离出具抗发炎活性成分antrocamphin A，续利用LPS诱导小鼠巨噬细胞（RAW 264.7）产生发炎的模式，解析antrocamphin A之抗发炎机制，结果证实antrocamphin A确可有效抑制一氧化氮自由基和前列腺素的生成，透过蛋白质表现分析得知，antrocamphin A之抗发炎活性是经由抑制一氧化氮生成酵素和第二型环氧酵素的mRNA表现，进而抑制了一氧化氮生成酵素、第二型环氧酵素和核转录因子-κB等酵素之表现。

Lipases and chitinases are industrially useful enzymes, and have the potential applications for use in food industry.

脂质酵素、磷脂质酵素及几丁质分解酵素是工业上应用研究近年来倍受瞩目的重要酵素，其在食品工业上应用的潜力亦大。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。