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The regulatory elements of the tetracycline resistance operon of Eschenchia coli, Tet repressor and Tet operator, have served as the basis for the establishment of heterologous repression-based inducible system in many eukaryotes including yeast, plants, insects, mammals and parasitic protozoa, such as Trypanosomes, Entamoeba, Giardia, Toxoplasma, Leishmania and Trichomonas.

基于四环素抑制子(Tetracycline Repressor,TetR)和四环素操纵子(Tet Operator,TetO)相互作用的四环素可诱导转基因表达系统,已经广泛的应用于很多真核生物,包括酵母、植物、昆虫、哺乳类细胞和诸如锥虫、阿米巴、贾第虫、弓形虫、利什曼和毛滴虫的寄生原虫。

Researches on drosophila, mice and yeast also suggested that epistasis between nuclear and mitochondria affected significantly the phenotype of offspring.

细胞质效应在动植物中广泛存在,在模式生物果蝇、小鼠和酵母上的研究表明,细胞质与细胞核间的上位性互作效应对后代有明显的影响。

A new gene which was a putative ERG was selected and expressed in yeast and it is helpful to study the function of the downstream genes.

选取其中一条候选早期应答基因为后续研究对象,并进行蛋白质的酵母表达,为下游的基因功能研究奠定一定的基础。

This paper introduces the current status and development tendency of the research and production of erythritol, and discusses the biosynthetic pathway of erythritol produced by an osmophilic yeast.

现介绍赤藓糖醇的生产和研究现状,耐高渗酵母赤藓糖醇的合成途径和赤藓糖醇的应用,并对其研究和发展提出看法。

Here, we investigated heterochromatin and euchromatin profiles of the entire fission yeast genome and explored the role of RNA interference in genome organization.

这里,我们对裂殖酵母整个基因组上的异染色质和常染色质的状态进行了研究,并探索了RNA干扰在基因组组织中的作用。

Hours, the cells were synchronized in G1 phase, for that α factor can induce the expression of Farl protein, which inhibited CDK activity.

将同步于G1期的酵母细胞转入含有HU的YPG培养基,细胞摆脱α因子的作用,开始进入S期进行DNA复制。

The fermentability of Pichia stipitis NLP31 was hight on the fermentation medium with less expensive inorganic nitrogen source, thus reducing the cost of the fuel ethanol production.

树干毕赤酵母NLP31在以廉价的无机N源为发酵培养基上的乙醇发酵性能高,能够降低燃料乙醇的生产成本。

In order to further confirm its subcellular localization , pYES2.0-MxIRT1::GFP with a inducible promoter was constructed and then transformed into the yeast strain by LiAc method. We observed the cells under the fluoroscope and laser confocal microscope through a method of Fm4-64, red and green fluorescent co-localized in plasma membrane.

根据结构预测MxIRT1是一种膜蛋白,为进一步确认它的亚细胞定位,利用构建好的诱导型酵母表达载体pYES2.0-MxIRT1∷GFP,通过GFP与活细胞荧光膜染料FM4-64结合的方法在荧光显微镜和共聚焦激光显微镜下观察。

The result indicated that increasing the dosage of Daqu could effectively reduce the formation of fusel oil, and adding appropriate proteinase, saccharifying enzyme and dry yeast could reduce the formation of fusel oil with effects decreasing in turn.

结果表明增加大曲用量可以非常有效的降低杂醇油的生成,添加适量蛋白酶、糖化酶或干酵母都可以降低杂醇油的生成,并且作用效果依次降低。

The characteristic of pichia pastoris host system was also discussed. The construction of recombinant strain with coding glycolate oxidase gene was thought of possessing great practical and academic value.

同时介绍了本研究拟采用的毕赤氏酵母表达体系的特点,论述了构建含有乙醇酸氧化酶基因工程菌的重要理论和应用价值。

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