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First,through monofactorial experiment,we studied the influences of pH,temperature and substrate concentration on acidic trehalase activity of baker's yeast.

首先通过单因素实验研究了pH值、温度、底物浓度对面包酵母酸性海藻糖酶活性的影响,然后通过正交试验得出了酸性海藻糖酶活性测定的适宜条件:海藻糖浓度1.6%、温度30℃、pH 4.0、酵母菌泥接种量为0.02g/mL。

First, through monofactorial experiment, we studied the influences of pH, temperature and substrate concentration on acidic trehalase activity of baker's yeast. Then we designed an orthogonal experiment to detective the optimal condition of ascertaining acidic trehalase activity. The optimal conditions are: substrate concentration 1.6%, pH 4.0, temperature 30 and seed volume 0.02g/mL compressed yeast.

首先通过单因素实验研究了pH值、温度、底物浓度对面包酵母酸性海藻糖酶活性的影响,然后通过正交试验得出了酸性海藻糖酶活性测定的适宜条件:海藻糖浓度1.6%、温度30℃、pH 4.0、酵母菌泥接种量为0.02g/mL。

When components of fermentation medium were glucose 20 gL^(-1), yeast extract powder 25 gL^(-1), MgSO4.7H2O 0.38 gL^(-1), L-aminothiopropionic acid 1 gL^(-1), Triammonium cetrate 2 gL^(-1), sodium acetate 2.5 gL^(-1) and tween 80 1 mlL^(-1), the production of bacteriocin reached the maximum and the diameter of bacteriostasis circle was 28.97 mm.

当培养基的组成为:葡萄糖20 gL^(-1),酵母浸粉25 gL^(-1),MgSO4.7H2O 0.38 gL^(-1),L-半胱氨酸1 gL^(-1),柠檬酸三铵2 gL^(-1),乙酸钠2.5 gL^(-1),吐温80 1 mlL^(-1)时细菌素的产量最大,抑菌圈直径可达28.97 mm。

This reflected that EMP and HMP pathways and the tricarboxylic acid cycle were very important to the bioreduction.

表明糖酵解途径、磷酸戊糖途径和三羧酸循环的顺利进行对于面包酵母在有机介质中催化4-氯乙酰乙酸乙酯还原反应都是十分重要的。

Using a batch culture, a maximum nuclease yield of 3500 U/mL was obtained with a complex medium characterized by high concentrations of yeast extract, tryptone and magnesium ion.

在批次酦酵中,使用复合培养基可得最大的核酸分解酵素产量3500 U/mL;此复合培养基之特点为含有高浓度的酵母萃出液、蛋白胴、镁离子。

The optimal shaking flask fermentation conditions were: initial pH 5.5,inoculum 15%,volume charge 135 ml in 250 ml flasks,and nitrogen source 10 ml mixed with 10 g/L yeast extract and 20 g/L tryptone.

摇瓶分批发酵工艺条件的最佳组合是:起始pH5.5,接种量15%,装液量135mL,氮源加入量为10mL含有10g/L酵母膏和20g/L蛋白胨的有机氮源。

ABSTRACT Candida tropicalis Y20-1 with a strong ability of assimilating starch and trichoderma virid T2 with an ability of degrading cellulose and semi-cellulose were used for mixed culture in production of feed-protein from distiller's grain.

以大曲酒糟为原料,采用同化淀粉能力强的热带假丝酵母Y20-1和有降解纤维素和半纤维素能力的绿色木霉T2混合发酵,生产蛋白饲料。

We first found out the preparation of intact budding yeast nuclei suitable for substrates for replication in Xenopus egg extracts.

我们首先摸索出了一个制备适用于爪蟾卵复制底物的完整芽殖酵母细胞核的方法。

Methods The fluconazole, amphotericin B and itraconazole susceptibility test were performed by Rosco disk diffusion method and NCCLS M27A broth macrodilution method with 110 yeastlike fungus strains isolated from clinical elderly patients specimens. 3 standard strains were used as the quality control.

应用 Rosco 纸片扩散法和美国临床实验室标准化委员会M27A宏量肉汤稀释法测定临床分离的110株老年病例中不同酵母样真菌菌株对氟康唑、两性霉素B、伊曲康唑的药敏状况,并以3株酵母菌株作质量控制。

With yeast autolysis technology,the harvested yeast cells were autolyzed for soy sauce production.The results showed that when the soy sauce wa...

结果表明:利用酵母自溶液配制的酱油,其全氮达到1.50g/100mL,氨基酸态氮达到0.78g/100mL,同时酱油的鲜味也得到了明显提高。

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