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In this paper, a high-yield cell-surface phytase yeast was studied to build foundation for deep study.

本文对一株高产细胞表面植酸酶酵母突变株WZ-4 菌的细胞植酸酶进行了研究。

The purpose was to study constitutive expression of the Phytase gene from Thermomyces sp.

[目的]研究植酸酶基因在毕赤酵母中的组成型表达。

Objective To explore the effect of strategy for methanol feeding during high density fermentation of Hansenula polymorpha on the expression of recombinant HBsAg.

目的 考察不同甲醇流加策略对汉逊酵母高密度发酵表达重组乙型肝炎表面抗原的影响。

GAP gene was found to be a good internal control for the first time for studying gene expression in H. polymorpha. It was shown by Northern hybridization that H-OLE1 gene was expressed in H.

为研究H-OLE1基因的转录及其调节规律,通过一系列实验,首次找到了可在研究多形汉逊氏酵母基因表达时用作内标的GAP基因。

To prove the H-OLE1 coding for △9-fatty acid desaturase, DNA electroporation system of H. polymorpha was established, and genetic complementation test was conducted.

为验证H-OLE1基因的功能,建立了多形汉逊氏酵母DNA电穿孔实验系统,进行了遗传互补测验。

Bio-active α-hydroxynitrilelyase expressed in Hansenula polymorpha after 0.5% methanol induction was obtained with 2.015 U determined by analysis of enzyme activity and SDS-PAGE.

酶活测定和SDS-PAGE分析显示HNL在汉逊酵母NCYC495(leu1.1)中得到正确表达,每毫升发酵液中获得2.015 U的醇腈酶。

There is a highly efficient metabolism for the utilization of the one-carbon compound methanol in methylotrophic yeast species, such as Candida boidinii, HansenuLa polymorpha and Pichia pastoris.

甲基营养酵母如Candida boidinii,HansenuLa polymorpha和Pichia pastoris具有利用一碳化合物甲醇的高效代谢机制。

The fusion gene was inserted downstream to the MOX promoter of Hansenula polymorpha shuttle plasmid pDGXHP2.0, and the constructed multicopy secretory recombinant plasmid was transformed to Hansenula polymorpha ATCC34438 Ura3(superscript - by electroporation.

将融合基因插入汉逊酵母穿梭质粒pDGXHP2.0的甲醇氧化酶基因启动子下游,构建成多拷贝分泌型重组表达载体。

But there is no report about the research using YC2.1 in Schizosaccharomyces pombe.

本文首先构建了含YC2.1基因的粟酒裂殖酵母表达质粒pESP-YC2.1。

Pombe. An active recombination of pREP3X-HA-mid2 was identified from 41 single clones of relevant transformed S.

在41个酵母转化单菌落中,筛选到一个有表达活性的pREP3X-HA-mid2重组子。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。