酵母的

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

The positive effects include invert of sugar into alcohol by yeast and invert of malic acid into lactic acid by lactobacillus etc.

摘 要：在整个葡萄酒的酿造生产过程中，不同的微生物起着不同的作用，酿酒酵母将糖转化成酒精，乳酸菌会将苹果酸转化成乳酸等，这些是正常的发酵过程；而醋酸菌会使葡萄酒发生酸败，产膜酵母会使葡萄酒起膜浑浊，霉菌会影响酒的风味等，这些是有害微生物的作用。

Experiment of the third part use the first part"s production as carrier, selecting Geotrich candidum and Candida tropicalis of higher production of protein also selecting producing the cellulose enzyme Bacillus subtilis FS and Lactobacillus bulgaricus by ourselves separated, design orthogonal test and a series of single factor experiment, finally assurancing the inoculation quantity of four strains germ, is Geotrich candidum 5%, Candida tropicalis 5%,Bacillus subtilis FS 5% of FS, Lactobacillus bulgaricus 3%, the most suitable development temperature is 30 "C , the development time is 15 days.

实验的第三部分是以第一步共培养所得的培养物为载体，选择产蛋白量高的白地霉、热带假丝酵母及本室筛选的产纤维素酶芽孢杆菌FS和自行分离的保加利亚乳酸杆菌，设计正交实验和一系列单因素实验，最终确定了四株菌的接种量，分别为白地霉5%，热带假丝酵母5%，FS5%，保加利亚乳杆菌3%，最适培养温度为30℃，培养时间为15天。

Chicken meal, brown rice, rice, corn, corn gluten meal, chicken fat, chicken, natural chicken flavor, pea fiber, rice flour, rice hulls, wheat gluten, dried beet pulp, soya oil, sodium silico aluminate, dried brewers yeast, fructo-oligosaccharides, anchovy oil (source of EPA/DHA), calcium sulfate, salt, potassium chloride, dried egg product, choline chloride, DL-methionine, taurine, Vitamins [DL-alpha tocopherol acetate, L-ascorbyl-2-polyphosphate, niacin supplement, biotin, riboflavin (vitamin B2) supplement, D-calcium pantothenate, vitamin A acetate, pyridoxine hydrochloride (vitamin B6), thiamine mononitrate (vitamin B1), folic acid, vitamin B12 supplement, vitamin D3 supplement], sodium tripolyphosphate, tea, Trace Minerals , L-Carnitine, preserved with natural mixed tocopherols and citric acid, rosemary extract.

能看懂的最好，我用翻译软件翻译一下是这样的鸡肉粉，大米，鸡肉脂肪，玉米蛋白粉，鸡肉，玉米，天然鸡味，干蛋产品，干甜菜粕，干燥啤酒酵母，豆油，凤尾鱼油（来源的EPA / DHA的）， 1赖氨酸，纤维素粉，盐，氯化钾，钠硅铝酸盐，果糖低聚糖，干啤酒酵母提取物，氯化胆碱，牛磺酸，维生素[消旋-α生育酚醋酸酯， L -抗坏血酸- 2 -聚，生物素，烟酸的补充，核黄素（维生素B2 ）的补充， D -泛酸钙，盐酸吡哆醇（维生素B6 ），维生素A醋酸酯，维生素B1单（维生素B1 ），叶酸酸，维生素B12的补充，补充维生素D3 ] ，微量矿物，万寿菊提取物， L -肉碱，保存与自然混合生育酚和柠檬酸，迷迭香提取物

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

After the identification of SARs in Attacus ricini rRNA gene, its rRNA gene and NTS were also found to have the ability binding with the nuclear scaffold of Saccharomyces cerevisiae. The 1kb NTS fragment which can bind clearly with nuclear scaffold of Attacus ricini was the chief fragment binding with yeast nuclear scaffold. This indicated the homology between this two kinds of SARs and provided an evidence for the evolutionary conservation of SARs.

在鉴定蓖麻蚕rRNA基因中具有骨架结合区域之后，利用体外结合的方法发现，蓖麻蚕rRNA基因及其非转录间隔区片段也具有与酵母核骨架结合的能力，尤其是能与蓖麻蚕核骨架结合的非转录间隔区1kb片段，也能明显地结合于酵母核骨架，这一结果表明二者的SAR顺序具有一定的同源性，为骨架结合区域具有进化保守性提供了又一证据。

Gibel carp were inoculated with formalin killed Aeromonas hydrophilaand fed with diets supplemented with appropriate amount of yeast β-glucan. 28 days after the inoculation, the infulence of dietary yeast β-glucan on the resistance of gibel carp against Aeromonas hydrophila were determined by examing their gained weight, agglutinating antibody titer, lysozyme activity, phagocytic activity and relative percent survival by challenging with live A.

在饲料中添加定量的酵母β-葡聚糖，投喂经注射接种福尔马林灭活的嗜水气单胞菌(Aeromonas hy-drophila)菌苗的异育银鲫28 d后，通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率，探讨了酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用。

Polymorpha cells in low level and produced a 1. 5kb transcript. Transcription of the gene was slightly repressed by exogenous △9 UFA. The FAR element in S. cerevisiae OLE1 promoter described by Choi et al. might not be crucial for positive transcriptional regulation of H-OLE1 gene.

Northern杂交发现，H-OLE1基因在细胞中以较低水平表达，产生1.5kb的转录子；基因表达略受不饱和脂肪酸的抑制；在多形汉逊氏酵母H-OLE1基因的转录调节中，Choi等在酿酒酵母OLE1基因中发现的脂肪酸调节元件FAR可能不是关键的。

Within plenty of systemic experiment just like the ability of fermentation test and the characteristic of fermentation test, F2 was screened as the best strain used in apple wine fermentation.

以实验室保藏的酵母J1，J2，M1，M2，F1，F2为出发菌株，通过测各菌株对苹果汁的发酵能力、发酵特性及发酵所得苹果酒品质的系统实验，选出F2为最佳苹果酒酿酒酵母，其对苹果汁的发酵能力强、发酵特性好，发酵所得苹果酒品质优，且酒体澄清透明、色泽橙红，具有苹果酒的典型风味，适用于果酒生产。

The reaction catalyzed by DAK converts DHA into dihydroxyacetone phosphate which is no toxic and can be ustilized by yeast cells. DAK presents in most organisms. DAK genes in the Saccharmyces cerevisiae and P. pastoris were cloned and characterized by a combined genetic and biochemical approach and the proteins encoded by these DAK genes are homodimer.

二羟基丙酮对酵母细胞来说是种有毒的化合物，在酵母菌中二羟基丙酮激酶参与二羟基丙酮的脱毒作用，DAK催化的反应使二羟基丙酮磷酸化，形成无毒性的磷酸二羟丙酮，可为酵母细胞所利用。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。