酵母的

The results showed that:(1) 5'-nucleotide contents in yeast autolysate was slightly increased by adding RNase frommalted barley roots;(2) higher pH did not improve the 5'-nucleotide contents even in the presence of RNase from malted barleyroots, but at the pH 8.0, the addition of Acalase could increase the final yield, soluble protein contents and 5'-nucleotide inthe presence of RNase from malted barley roots.

酵母核酸酶的pH稳定性和温度稳定性均比麦芽根核酸酶的要差。这说明自溶法生产酵母提取物时，即使外加5'-磷酸二酯酶不能有效提高呈味核苷酸的原因并非由于外加的5'-磷酸二酯酶性质不稳定，而是另有其它原因。

Yeast elicitor repeatedly added on days 15, 17 and 19 at the dose of 106 mg total sugar/1 medium improved PeGs accumulation further, and the final PeGs content and production reached 31.3 mg/g DW and 317.8 mg/1, which were 195%and 235% that of the control, respectively.

研究表明酵母诱导子的添加同时促进了细胞生长和PeGs合成，在培养第15、17和19天以106mg总糖／l的浓度三次加入酵母诱导子极大地刺激了PeGs的合成，其含量和产量在21天分别达31.3mg／g DW和317.8 mg／l，为对照培养的195％和235％。

The erythritol is produced industrially by Zhongshi Gerui biotechnology Co., Ltd., Zibo by means of utilizing Candida lipolyti developed by China Food Fermentation Industry Inst. And with the preservation number of CGMCC No.1431 as the high erythritol yield spawn and deep liquid fermentation of glucose. The erythritol is used as functional sweetening agent in sugar substitute for diabetics, low calorie sugar, low calorie beverage, low calorie cake, low calorie candy, low calorie milk product, etc.

具体是指淄博中食歌瑞生物技术有限公司与开发出赤藓糖醇高产菌种――解脂假丝酵母 Candida lipolytic 的中国食品发酵工业研究院合作，利用保藏编号为 CGMCC No.1431的解脂假丝酵母 Candida lipolytica 菌种，用葡萄糖深层液态发酵，工业规模制取的赤藓糖醇；作为功能性甜味剂，在下述产品中的应用：糖尿病人代用糖，低热量佐餐用糖，低热量饮料，低热量糕点，低热量糖果，低热量奶制品和低热量冰激淋或低热量雪糕或低热量冰糕。

The results indicated that the content of cytosolic CaM in cells treated with exogenous Ca2+ has increased indistinctively, while fluorescence intensity in cells treated with TFP decreased. So we believed that exogenous Ca2+ has little effect on the expression of CaM. High concentration of TFP can enter yeast cells and combine to CaM to make it inactive, which is the reason that TFP restrain the growth of yeast cells. 5mmol/L EGTA could completely arrest the cell proliferation of MFP7 after 28h, when the fluorescence intensity in cells wasobviously increased with flow cytometry and LSCM.

被较高浓度TFP处理过的MFP7胞内荧光强度则相对较弱，推测是因为TFP进入胞内与CaM结合从而使其失活，这也是高浓度TFP抑制酵母细胞增殖的原因。5mmol／L EGTA处理28h左右后，酵母细胞的生长被抑制在G1晚期，此时通过细胞流式法和在激光扫描共聚焦显微镜下观察到胞内荧光强度显著高于对照，说明CaM的表达水平在G1后期开始上升；回加10mmol／L Ca~(2+)处理24h后，细胞开始恢复生长，细胞流式法测定胞内荧光强度有所下降，表明多数细胞完成G1／S转换，进入生长对数期。

In the condition of the above mentioned, field experiments were done. The results showed that:The process was the inoculation proportion of Candida tropicalis, Pseudo monas and Candida utilis as 1:2:2, inoculation 2%, 2.0% urea added in Jowar juice, pH 6.0, fermenting 72h at 30℃. The content of fulvic acid could reach more than 20%.5% mixture of Azotobacter chooroccum, Phospho bacteria and Silicate bacteria (2:1:2) were added to the product. The total number of bacteria reached 10 /ml.

研究结果表明：用热带假丝酵母、假单胞杆菌和产朊假丝酵母组合进行液体发酵，接种比例1∶2∶2，接种量2%，在甜高粱秸秆汁中加入2.0%的尿素，pH值为6.0，发酵温度为30℃，发酵72h，发酵产物中生化黄腐酸含量可达20%以上；将圆褐固氮菌、磷细菌、钾细菌以2∶1∶2的比例，按5%加入到发酵产物中，三种细菌总的活菌数可达10~8个/ml；生化黄腐酸复合生物液肥对各种作物的产量均有不同程度的提高，经济效益显著。

We transformed antiapoptotic genes Bcl-2, CED-9 and PpBI-1 into the yeast to evaluate their function of resistance to cold stress. The results are as follows:(1) Cold induced apoptosis in yeast cells, moreover, yeast cells display representive features such as chromatin condensation, DNA fragmentation and chromatin margination.

而且酵母没有如Bcl-2家族的主要凋亡调控基因的同源基因，因此酵母是研究外源抗凋亡基因Bcl-2，CED-9和PpBI-1在抗冷方面的作用及其调控机制的理想体系。

In this paper,Growth hormone cDNAs of 9 Pleuronectiformes fishes were cloned and the phylogenetic analysis via these sequences was performed by PAUP software.Four vectors expressing flounder GH in Saccharomyces cerevisiae were constructed and studies on the transgenetic yeast were made.

本论文克隆了9种鲽形目鱼类的生长激素基因cDNA序列并运用PAUP软件进行了分子系统进化树分析，构建了四种不同类型的酿酒酵母表达牙鲆生长激素的载体，并研究了牙鲆生长激素基因在酿酒酵母中的表达情况。

In order to express the recombinant peptide of both gp52 and pp150 Oterminal peptides from human cytomegalovirus in the Pichia pastoris, the recombinant plasmids were transformed into Pichia pastor is by electroporation after linearised respectively by Sac I or Bg1II, the transformants were screened in MD plates without histidine.

为了在巴斯德毕赤酵母中表达人巨细胞病毒gp52C末端和pp150C末端串联片段的嵌合肽，将构建好的hCMVp-pPIC9K重组质粒分别经Sac Ⅰ和Bg1 Ⅱ酶切，电打孔法导入毕赤酵母GS115后，在缺组氨酸的MD板上筛选出转化子。

In this paper, the principle of preparation of live vaccine with Saccharomyces cerevisiae by using the surface-display technique, the advantage of displaying protein on surface of the yeast cell, and surface-display of haemolysin from Vibrio harveyi on the yeast cells and their potential applications as live vaccine in marine fish are reviewed.

本文对利用酒精酵母细胞表面展示技术制备活疫苗的基本原理，在酵母细胞表面展示蛋白质的优点，以及哈维氏弧菌溶血素蛋白在酵母菌细胞表面上展示及其作为活疫苗的潜在应用作了综述。

This paper describes two-step enrichment and double-time replica plating technology to select mutants with enhanced α,ω-oxidation and blocked β-oxidation. After C.

为提高热带假丝酵母转化烷烃生产长链二元酸的能力，建立了通过两步浓缩和双重影印技术筛选获得α、ω-氧化增强、β-氧化减弱的热带假丝酵母诱变菌株的筛选体系。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。