酵母的

The glucoamylase I gene was inserted into the pAC1 plasmid,and a expression plasmid was constructed,then it was used to transform the yeast strain, S.cerevisiae AS2.1364,without auxtrophic marker,The amylolytic tests showed that when this transformant was incubated on plate of YPDS medium containing 1% glucose and 1% starch at 30℃ for 48 hrs zones of starch degradation could be visualized by stained with iodine vapour.

这说明酵母转化子不仅表达了该酶，且有效地分泌到胞外，从而水解培养基中的淀粉，进一步测定酵母转化子48hrs发酵液的葡萄糖淀粉酶活力为8.4u/ml，培养基中的淀粉已完全水解，其生物产量较高。

Strains of high alcohol-producing or high ethyl acetate-producing yeasts were isolated from koji of Shanxi overmature vinegar at different temperatures. One high alcohol-producing yeast Nz19.3 and one high ethyl acetate-producing yeast Nz9.8 were identified as Saccharomyces cerevisiae and Hansenula anomala, respectively.

通过对山西老陈醋大曲的富集培养，筛选到9株高产乙醇或高产乙酸乙酯的酵母菌功能菌株，分别挑选高产乙醇和乙酸乙酯的酵母菌各1株（经分类鉴定，二者分别为酿酒酵母和异常汉逊酵母）进行共培养试验。

With the development of molecular biology, based on yeast two-hybrid, many techniques such as yeast one-hybrid, reverse two-hybrid, three-hybrid, and extranuclear two-hybrid were set up to be applied in the research on the interaction between protein-protein, protein-DNA, protein-RNA, and protein-ligand.

随着分子生物学的发展，在酵母双杂交的基础上又建立起了酵母单杂、反向双杂交、三杂交及核外双杂交等多项技术，并已经成功地运用于蛋白质之间、蛋白质与DNA、RNA和配体之间相互作用的研究。

The CCAAT binding factor of brewer's yeast Saccharomyces cerevisiae has been shown previously to be a heteromeric complex containing HAP2, HAP3, HAP4 and HAP5 subunits. This factor can bind specifically to CCAAT-containing upstream sites within the promoters of numerous yeast genes, and then activate the transcription.

HAP转录因子(HAP2/HAP3/HAP4/HAP5)是存在于酿酒酵母中的一种异源多聚蛋白，它能与酵母中许多启动子上游的CCAAT盒专一性结合，以增强基因的转录。

The CCAAT binding factor of brewers yeast Saccharomyces cerevisiae has been shown previously to be a heteromeric complex containing HAP2, HAP3, HAP4 and HAP5 subunits. This factor can bind specifically to CCAAT-containing upstream sites within the promoters of numerous yeast genes, and then activate the transcription.

HAP转录因子(HAP2/HAP3/HAP4/HAP5)是存在于酿酒酵母中的一种异源多聚蛋白，它能与酵母中许多启动子上游的CCAAT盒专一性结合，以增强基因的转录。

Objective: On the basis of the random mutagenesis library of ARS, we wish to preparation by screening new ARS sequence which not only have higher ARS activity, but also display higher transformation frequency, in order to constructed new gene recombination vector which have higher efficiency in the Nonconventional yeast expression system.

目的：通过构建酵母ARS 随机突变体库，从中筛选出具有较强的转化效率的ARS序列，用以构建新型高效的克鲁维酵母基因工程载体。

The recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened and cultured in YPD, BMMY and MM media respectively. The expression levels of HBsAg were determined by ELISA, and the virus-like particles were observed by transmission electron microscopy.

将其电转化多型汉逊酵母尿嘧啶缺陷型宿主菌ATCC34438(Ura3-)，筛选分泌表达HBsAg的汉逊酵母工程菌株，并比较在YPD、BMMY、MM3种培养基中分泌表达HBsAg的水平，ELISA检测培养上清液中HBsAg的表达量，透射电镜观察类病毒颗粒。

MOTIVATION: In order to facilitate a systematic study of the promoters and transcriptionally regulatory cis-elements of the yeast Saccharomyces cerevisiae on a genomic scale, we have developed a comprehensive yeast-specific promoter database, SCPD.

动机：为了促进在基因组范围上对酵母中启动子和转录调控顺式调控元件的系统性研究，我们开发了一个全面的酵母特异的启动子数据库----SCPD。

However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

而传统的三丁酸甘油酯平板缺乏有效营养成分，重组酵母无法生长；橄榄油平板灵敏度较低，低脂肪酶活力的重组酵母不能产生变色圈，这给产脂肪酶基因工程菌的鉴定带来了困难。

According to the nutritional needs of B.bifidum,based culture and stimulated factors were optimized by quadratic design.The optimum based culture was as following: yeast extract was 0.9%,tryptone was 0.3%,soya peptone was 0.3%,glucose was 2.0%,bifidus was 0.8%,growth factor was 10%.

针对两歧双歧杆菌的营养需要，采用正交试验设计优化增殖培养基，结果表明，酵母膏是影响短双歧杆菌的显著因素，最佳的优化培养基配比是：酵母膏0.9%，胰蛋白胨0.3%，大豆蛋白胨0.3%，葡萄糖2.0%，双歧因子0.8%，生长因子10%。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。