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Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库,在大肠杆菌中扩增后提取混合质粒DNA,经电转化非缺陷标志酒精酵母 AS.2.1364,在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子,从阳性转化子中分离重组质粒证实含5.0kb的插入片段,用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

The diarrhea frequencies and the diameters of egesta of the mice fed on opioid peptides in filter papers were decreased significantly (P.05), under feeding conditions, there is less diarrhea than that under fasting conditions, there is a obvious difference between sample group and controlled group.

饥饿状态下测定的粪便水圈直径结果表明,阿片肽组小鼠粪便在滤纸上的水圈直径比对照酵母组显著降低(P.05)。进食状态下试验各组的腹泻程度比饥饿状态下都有所降低,灌胃阿片肽2mg/只组与对照酵母组之间有显著差异(P.05)。

Effect of SMF on Microorganisms (1) The influences of magnetic induction in SMF on microorganisms were studied.

以细菌、酵母和霉菌为对象,研究液体中不同强度静态磁场对微生物的影响,获得磁场作用24h后微生物的存活率与磁场磁感强度的拟合方程如下:(1)啤酒酵母〓(2)黑曲霉〓(3)枯草杆菌〓 2。

An SCP powder called Provesteen, now on the market, is produced from a yeast that grows at densities similar to those achieved by centrifugation with ordinary strains. This means not only that centrifugation can be omitted, but also that there is no excess water to be recycled; the yeast goes right from the fermenter to a pasteurizer and a drier.

一种叫Provesteen的SCP粉末现在已进入市场,它能在一种从普通酵母离心分离后才能达到的密度条件下生长,这就意味着,它不仅可省略掉离心步骤,而且也不需要回收过量的水;这些酵母直接从发酵罐到巴氏消毒器和干燥器。

There are many different species of yeast but the one normally encountered in the distilling and brewing industries is called Saccharomyces Cerevisiae.

酵母有许多不同的种类,但是在蒸馏和酿造业中最常见的则是一种叫做Saccharomyces Cerevisiae的酿酒酵母。

This methods of sensory evaluation and Thiobarbituric acid value were used to study the effect of active carbon, cyclodextrin, yeast on the fishy flavor's removing of the acid-/alkali-isolated silver carp protein, furthermore, study the mechanism of the fishy flavor's removing by yeast, using the simultaneous distill extraction technology.

通过感观评定,测定硫代巴比妥酸值,研究了酸碱法对鱼肉蛋白腥味的影响及活性炭、环糊精、酵母发酵的脱腥效果,并应用蒸馏萃取-气相-质谱技术研究了酵母发酵对碱提蛋白风味的影响及脱腥机理。

Gather a sour buffer liquid of the sugar adoption lemon to carry on taking out to lift to the sweet dew in the beer leaven, separate many sugar from ferment a liquid, is precipitate with cold methyl alcohol, get a white thick lift a thing, use the 铵 salt and boric acid of the quarter again with many sugar the formation match with a thing, adding ether to precipitate, getting white and refined sample, average each 50 gs the wet leaven cell can get such refined sweet dew to gather the sugar 0.6 gs, computing with the this kind method withdraw of produce a rate for 1.2%.

对啤酒酵母内的甘露聚糖采用柠檬酸缓冲液进行抽提,从发酵液中分离多糖,以冷甲醇沉淀,得白色粗提物,再用季铵盐和硼酸与多糖形成配合物,加乙醇沉淀,得到白色精制样品,平均每50g湿酵母细胞可得到这样的精制甘露聚糖0.6g,计算出以此种方法提取的产率为1.2%。

To investigate the mechanism of tTG in cell apoptosis, yeast two hybrid system was used to screen HeLa cDNA library.

为研究tTG在细胞凋亡过程中发挥作用的机制,利用Gal 4酵母双杂交系统筛选了HeLa细胞中与tTG相互作用的蛋白质,获得了 17个阳性酵母克隆。

The continuous ethanol fermentation with mixed substrates consisting of glucose and xylose in two series-wound fixed columns packed with gel beads of immobilized cells was developed and tested in the experiment.

本研究采用海藻酸钙固定普通酿酒酵母细胞和嗜鞣管囊酵母细胞于两个串联的发酵罐内,连续发酵葡萄糖和木糖组成的糖液并与膜耦合来制取酒精。

The effect of aeration rate was studied in the culture of brewer's yeast used for ATP production.

结合酵母细胞酶促合成ATP的活性测试,在5L发酵罐中培养啤酒酵母,考察了通气对菌体生长和细胞活性的影响。

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