酵母的

France Lesaffre Group Company is one of the world famous multinational, who is founded 1853 that 150 years before and now is in the leadership in world yeast area, the Yeast and Baking Ameliorant and Extract Yeast are the 1st in the world, and the malt is the 3rd in the world.

法国乐斯福公司是世界上著名的跨国公司之一，创建于1853年，在食品酵母领域处于世界领先地位，乐斯福集团酵母居世界前列，烘焙改良剂居世界前列，酵母精居世界前列，麦芽居世界前列。

Furfur. At the same time, selected the culture supernatant. Used the whole-fat-cow"s-milk plate method, the Folin-phenol method and the QuantiCleave proteinase Assay Kit to test the proteinase activity of mycelial phase and yeast phase of M. furfur. The effect of temperature and pH on this assay was also evaluated.Result:①The whole-fat-cow"s-milk plate method: Seven days after inoculation, the proteinase activity of the mycelial phase supernatant (12.5 ± 1.2mm) is higher than that of the yeast phase (9.25±0.71mm)(p.05); With the passage of the inoculation time, the proteinase activity of the mycelial phase is rising; the temperature has no obvious effect on the proteinase

结果：①全脂牛奶平板法：培养第7天菌丝相上清液蛋白酶活性(12.5±1.2mm)高于酵母相(9.25±0.71mm)(p＜0.05)；菌丝相上清液蛋白酶活性随培养天数延长而增大；25℃和37℃两种温度对上清液蛋白酶活性无明显影响(P＞0.05)；②Folin-酚法：上清液蛋白酶活性随培养天数、菌落计数、菌丝诱导率增加而增大，培养液pH值呈下降趋势；同一天的菌丝相上清液蛋白酶活性明显高于酵母相(p＜0.05)；三种温度(25℃、37℃、49℃)及三种pH值(3.8、5.6、7.4)对上清液蛋白酶活性无明显影响；温度37℃、pH5.6时菌丝相上清液蛋白酶活性最高；③QuantiCleave蛋白酶试剂盒：菌丝相及酵母相上清液中均含有蛋白酶且

Construction yeast and plant expression vector with original gene and mutated gene, transferred into tobacco plant by electroporation and Agrobacterium mediated transformation respectively, validating genic function. Under the glyphosate pressure, the transgenic yeast strains with good tolerance are screened. The mark named ATME-4-M and ATME-9 prives the attained genes have biological function.

成功地将epsps基因构建到酵母胞内表达载体pPIC3.5K和植物表达载体PBI121上，利用电击法转化酵母及农杆菌介导法转化烟草，转基因酵母在草甘膦压力下筛选出耐受性较好的菌株，标记为ATME-4-M和ATME-9证明了所获基因具有生物学功能。

Yeast cell rupture conditions by zymolysis were determined with waste brewer's yeast as material and α-amino nitrogen as inspection target.

确定了以啤酒废酵母为原料，酵母酶解液中α-氨基氮含量为考察指标的蜗牛酶酶解破壁条件；并以啤酒废酵母酶解液为有机氮源，厌氧发酵制备丁二酸。

High palmitoleic acid-producing strains were obtained by protoplast fusion between Saccharomyces cerevisiae No.12.926 producing palmitoleic acid and Rhodotorula No.12.908 producing oil and fat.

采用原生质体融合技术进行产棕榈油酸酵母Saccharomy cescerevisiaeNo.12.926和产脂酵母RhodotorulaNo.12.908的融合研究，获得了棕榈油酸高产酵母工程菌株。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

Three capsules or tablets of brewer's yeast three times per day for two weeks was reported to improve three cases of infectious diarrhea caused by Clostridium difficile . 24 Animal research has confirmed that brewer's yeast helps fight this unfriendly bacterium. 25 Note that real brewer's yeast is not identical to nutritional, or torula, yeast and that when asking for "brewer's yeast" in health food stores, people are often directed toward these other products.

三胶囊或啤酒酵母片两个星期，每日3次报告，以改善的三名艰难梭菌引起的感染性腹泻病例。24动物的研究已经证实，啤酒酵母协助打击这种不友善的细菌。25（请注意，真正的啤酒酵母不相同的营养，或torula，酵母和当为&啤酒酵母在保健食品商店&要求，人们往往直接针对这些其他产品。

FDR3 was inserted into the yeast expression vector pDBLeu and then was transferred to the yeast strain M3 mediated by PEG/LiAc and by electroporation . In the medium without copper and iron, onlywith ferric citrate , the expression of FDR3 could complement the growth defect of ctrl mutant .

把FDR3基因片段克隆到酵母表达载体pDBLeu上，然后通过电击和乙酸锂转化两种方法将重组质粒导入酿酒酵母Ctrl突变体（M3 mutant）中，采用酵母异源互补法进一步鉴定FDR3基因的功能。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

Pombes growth inhibition experiment. The difference has statistic significance (P＜0.01) between these cell treated with 5.0μM, 10.0μM and 20.0μM edelfosine for 6 h and those cell treated with 0μM edelfosine for 6 h.

利用粟酒裂殖酵母细胞胞核分裂试验和粟酒裂殖酵母DNA含量检测试验，观察依地福新对粟酒裂殖酵母细胞胞核分裂的影响。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。