酵母的

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Used to make beer; and just as the yeast in a sourdough starter gives sourdoughbread its distinctive flavor, different types of beer yeast help to give beer itsvarious tastes.

酿造啤酒的酵母种类很多；就像酵母在酵头中使酵母面包有特殊风味一样，不同的啤酒酵母给啤酒带来了不同的风味。

The paper researched by 24 Saccharomyces cerevisiae strains and three kinds of related ascomycetous yeasts in the Angel Yeast Co.,L td,by electrophoresising and sequencing, ribosomal DNA ITS-1 sequences can analysis their's phylogenetic systematics relationship.

本论文以安琪酵母股份有限公司有代表性的酿酒酵母属中24株酿酒酵母及相关3种子囊菌酵母为研究对象，通过对所选取酵母菌株的核糖体DNA ITS-1序列进行电泳和克隆测序，分析出它们之间的系统学关系。

METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Experimental result makes clear, the bacili having breed that belongs to lactobacillus is belonged to, lacteal coccus is belonged to, streptococcic belong to and alvine coccus belongs to what; belongs to microzyme to yeast is belonged to or Han Xun finishs yeast of bare family name is belonged to, false silk yeast belongs to Debali yeast is belonged to etc.

实验结果表明，属乳酸菌的有乳杆菌属、乳球菌属、链球菌属和肠球菌属；属酵母菌的有汉逊酵母属或毕赤氏酵母属、假丝酵母属德巴利酵母属等。

Yeast extracts made from baker's, brewer's or torula yeast have now become key elements in the development of most savory flavor systems.

由面包酵母、啤酒酵母或圆酵母制成的酵母抽提物现已成为大多数调味品开发系统中的关键因子。

Sour mashes are fermented with both fresh and previously fermented yeast; sweet mashes employ only fresh yeast.

酸性糖化醪，是用酵母发酵的，这种酵母包含一部分先前发酵过的酵母；糖化醪则是用鲜酵母制得。

SUC2 mainly encodes sucrose invertase. The invertase could hydrolyse sucrose into glucose and fructose which are carbon source for yeast growth. When sucrose is used as the main carbon source, invertase is necessary for the growth of yeast. Accordingly, it is of great significance for yeast glycometabolism to study the sequence, transcription and regulation of sucrose invertase.

蔗糖转换酶基因(SUC2)编码蔗糖转换酶，通过降解酵母细胞外的蔗糖成果糖和葡萄糖来提供酵母生长所需的碳源，在以蔗糖为主要碳源的培养条件下，蔗糖转换酶对于酵母生长必不可少，因此研究蔗糖转换酶基因序列、转录、调控等对酵母蔗糖代谢具有重要意义。

Sour mash es are fermented with both fresh and previously fermented yeast; sweet mash es employ only fresh yeast.

酸性糖化醪，是用酵母发酵的，这种酵母包含一部分先前发酵过的酵母；糖化醪则是用鲜酵母制得。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。