酰胺酶

It is now well understood that asparagine synthetase is composed of two parts: a wedge-shaped place in the two antiparallel β-overlapping layers near the N-terminus that functions in glutamine hydrolysis; a C-terminus responsible for binding of Mg2+ ATP and aspartate.

现又明确天冬酰胺合成酶是由两部分组成：N基末端的两个反平行式β-折叠片层中的楔形部分是负责水解谷氨酰胺的活性部位；C基末端负责结合Mg2+ATP和天冬氨酸。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

Nicotianamine synthase gene has an important function in the Fe-deficiency response of graminaceous monocots, and it catalyzes substance to nicotianamine which plays an important carrier in the internal transport of Fe(superscript 2+) and other divalent metal ions. Nicotianamine can bind with Fe(superscript 2+) to form Fe(superscript 2+)-NA complex and protect plants from toxicity in acidic soil.

烟酰胺合成酶基因在单子叶禾本科的缺铁胁迫应答反应中起着关键作用，它的催化产物烟酰胺是铁及其他二价金属离子在体内吸收和转运的重要载体，且能与Fe(上标 2+)结合形成Fe(上标 2+)-NA复合物，从而使植物在酸性土壤中免受铁毒害。

The optimization of ferment, preparation of immobilized cells, optimization of bioconversion and extraction of nicotinamide were studied in this paper.

本论文中主要研究烟腈水合酶发酵工艺的优化、固定化细胞的制备、固定化细胞生物转化烟腈生产烟酰胺的工艺优化、烟酰胺的提取工艺。

In this study rat a-amidase was expressed secretively in S.

本研究在链霉菌中分泌表达了大鼠的α-酰胺化酶，以便利用链霉菌生产酰胺化多肽。

N-isopropylacrylamide, which has both hydrophilic acylamino and hydrophobic isopropyl, receives much concern in recent years due to its polymers showing favorable thermosensitivity and memory effect. It has been used extensively in drug delivery, material separation, enzyme immobilization and immunoanalysis.

N-异丙基丙烯酰胺同时具有亲水性的酰胺基和疏水性的异丙基，使其系列聚合物具有优良的温敏智能特性和记忆效应，目前已广泛应用于药物缓释、物料分离、免疫分析、酶的固定等领域。

The paper proposed a possible binding mode of MS-275, a benzamide histone deacetylase inhibitor, to HDAC by intensive docking study.

通过计算机模拟的对接过程研究，发现了MS-275--一种苯甲酰胺类的组蛋白去乙酰酶抑制剂与酶的可能的全新结合方式。

In order to clarify the rules of POD isoenzyme in the hazelnut, identify their blood relations between female, male and filial generations and select the superior parents for cross-breeding, we have studied the characteristics of peroxidase isoenzyme which are distributed in the 34 hazelnut varieties or lines by polyarclamide gel electrophoresis, The results showed that:POD isoenzymes of Corylus heterophylla Fisch, Corylus avellana L, and the hybrid of Corylus heterophylla Fisch × Corylus avellana L had obvious difference; some hybrids had lost P10 spectrum band of parents; the enzyme spectrum of 32 hybrid hazelnuts produced 'new hybrid spectrum bands'that could be considered as a mean to identify hybrids.

为了阐明过氧化物同工酶在榛品种中的分布规律，探讨平榛、欧榛及其杂种榛之间的亲缘关系，选育优势杂交亲本，本试验采用聚丙烯酰胺凝胶垂直电泳，研究了34个榛品种成熟叶过氧化物酶同工酶酶谱特征，结果表明：平榛、欧榛及其平欧杂种榛品种间酶谱具有较大差异；有的杂种品种遗失了父母本共有谱带P10；32个平欧杂种榛品种出现了双亲所不具有的新酶带，可作为鉴别杂种的一条途径。

In this paper,the Lactate dehydrogenase, malate dehydrogenase,superoxide dis-mutase of five tissues of Carassius auratus gibelio; Cyprinus carpio Linnaeus; Esox americanus Gmelin; Leuciscus waleckii in Dalai Lake were studied by vertical polypropylene gel electrophoresis and the isozyme composion of four kinds of fishes were obtained. Then the analysis of the expressing specify of 3 kinds of isozyme in different tissues and loci were carried on and the genetic base of isozyme system was discussed.

本实验采用垂直板聚丙烯酰胺凝胶的电泳方法对达赉湖的野生鲤鱼、银鲫、狗鱼、东北雅罗鱼的五种组织脏器(肌肉、心脏、肝脏、脑、眼睛)的三种同工酶(苹果酸脱氢酶、乳酸脱氢酶、超氧化物岐化酶)进行了初步研究，获得了达赉湖四种鱼类物种的同工酶谱，在此基础上对同工酶在不同组织的表达差异和基因位点进行分析，探讨其同工酶系统的遗传基础。

Glutamine-binding protein is one of the ligand-specific periplasmic binding proteins in the Escherichia coli permease systems, predicted to play an important role in transferring Gln from the pe-riplasmic space to the cytoplasmic space.

谷氨酰胺结合蛋白(Glutamine-binding protein， GlnBp)是大肠杆菌透性酶系统中一个细胞外液底物专一性结合蛋白，对于细胞外液中谷氨酰胺的运输和传递至关重要。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。