酰胺酶

The results showed that the soil catalase and dehydrogenase activities, soil microbial biomass N and microbial biomass C were restrain somewhat by four acetanilide herbicides at the beginning of cultivation, but can recover soon.

研究表明，培养初期丁草胺、乙草胺、丙草胺和异丙甲草胺对土壤过氧化氢酶和脱氢酶活性、对土壤微生物量氮和微生物量碳有不同程度的抑制，但均能很快恢复，四种供试酰胺类除草剂对土壤生态的影响不大，除草剂之间也没有表现出显著差异；培养初期rac-异丙甲草胺和S-isomer高浓度处理均比常规用药量处理对土壤酶、土壤BN和Bc的影响大。

Most of these peptides are amidated at their C-terminals, which is essential for their full activities. The a-amide comes from posttranslational modification that is catalyzed by α-amidase (α-AE or PAM). The amidation reaction is a rate-limiting step in the biosynthesis of regulatory peptides.

大部分活性肽的C-端是酰胺化的，而且α-酰胺基为多肽的完全生物活性所必需；α-酰胺基来自多肽的翻译后加工，由α-酰胺化酶催化完成，是酰胺化多肽生物合成途径中的限述步骤。

The absorption of root system was the main sources of nitrogen in the plant.The effects of matrix ventilation on the abaorption,transportion and metabolism of nitrogen in the root system of cucmber were discussed deeply.The results showed that contents of ammonium,nitrate,free amino acids and soluble protein in cucumber roots were higher in G1 treatment.Contents of ammonium,nitrate and free amino acids in cucumber roots were the trend of the dropping after the first rising in F1 treatment,but contents of soluble protein was lower11.03%than that of the control.

根系吸收是植物体内氮素的主要来源，本研究深入探讨了基质通气状况对黄瓜根系氮素吸收、运输和氮代谢的影响，结果表明G1通气处理根系的铵盐、硝酸盐、游离氨基酸和可溶性蛋白含量均较高；非通气处理(F1处理)根系内铵盐、硝酸盐和游离氨基酸含量呈先升高后降低的趋势，而可溶性蛋白含量始终较低，比对照降低11.03%。G1处理根系硝酸还原酶、亚硝酸还原酶、谷草转氨酶、谷丙转氨酶、谷氨酰胺合成酶活性均较高；而F1处理的酶活性在处理末期显著降低，其中谷氨酰胺合成酶活性比对照降低33.33%。

Antifolate Alimta derived from pyrrolo[2,3-d]pyrimidine is a multitargeted antitumor agent, it can inhibit at least five major folate-dependent enzymes: thymidylate synthase, dihydrofolate reductase, glycinamide ribonucleo -tide formyltransferase, aminoimidazole ribonucleotide formyl transferase and C-1 tetrahydrofolate synthetase(C1-S).

吡咯并[2，3-d]嘧啶类叶酸拮抗剂Alimta是一种多靶向的抗癌药物，它能抑制至少五种叶酸依赖性酶：胸苷合成酶、二氢叶酸还原酶、叶酰多聚谷氨酸合成酶、甘氨酰胺核糖核苷酸转甲酰酶及C1-四氢叶酸合成酶(C1-S)。。

Major procurement of goods: a three-chlorosilanes, dichloromethane, AE activity ester, 3-iodine Silane, special acid, pentyl chloride, triethylamine, thiadiazole, tetrazolylazo acid, 2 - Acetamide, tetrahydrofuran, the four-guanidine, isopropanol, five phosphorus trichloride, sodium vary bitter, acid, sodium phenylacetate, 6-2 silicon n-amine, Ethylacetoacetate , Methyl isobutyl ketone, potassium dihydrogen phosphate, aluminium oxide, DL methionine, N, N-dimethylaniline, NN-diethyl aniline, 4 sodium EDTA, Anhydrous sodium sulfate, ammonium sulfate, potassium sulfate, sodium acetate, sodium carbonate, DMC, formic acid, sodium chloride medicinal, oxalate, protopine, acetone, alcohol, acetic acid, vinegar Ethyl, butyl acetate, methanol, ethanol (anhydrous, industrial, medicinal), formaldehyde, Ye Jian (30%), hydrochloride (industrial-grade, refined grade, reagent level), sulfate (98%), Ammonia, calcium carbonate, chlorine dioxide, 6 - APA ,7-ACA ,7-ADCA ,7-ANCA, sulbactam, ceftazidime activity ester, Deng salt (hydroxymethyl-K, acid precursors Potassium, sodium dihydrogen methyl), resin, the enzyme, water treatment agent, Xiao Mo agent, demulsifier, flocculants, activated carbon, all kinds of medicinal materials, All kinds of additives

三甲基一氯硅烷、二氯甲烷、AE活性酯、三甲基碘硅烷、特戊酸、特戊酰氯、三乙胺、噻二唑、四氮唑乙酸、二甲基乙酰胺、四氢呋喃、四甲基胍、异丙醇、五氯化磷、异辛酸钠、苯乙酸、苯乙酸钠、六甲基二硅胺烷、乙酰乙酸乙酯、甲基异丁酮、磷酸二氢钾、三氧化二铝、DL蛋氨酸、N，N-二甲基苯胺、NN-二乙基苯胺、乙二胺四乙酸四钠、无水硫酸钠、硫酸铵、硫酸钾、醋酸钠、碳酸钠、碳酸二甲酯、甲酸、药用氯化钠、草酸、片碱、丙酮、正丁醇、冰醋酸、醋酸乙酯、醋酸丁酯、甲醇、乙醇、甲醛、液碱（30％）、盐酸（工业级、精制级、试剂级）、硫酸（98％）、氨水、碳酸钙、二氧化氯、6－APA、7－ACA、7－ADCA 、7－ANCA、舒巴坦、头孢他啶活性酯、邓盐(羟甲基钾、前体酸钾、二氢甲基钠)、树脂、生物酶、水处理剂、消沫剂、破乳剂、絮凝剂、活性碳、各种药用辅料、各种添加剂

The distribution, properties,structure and regulation of plant allantoinase were reviewed in detail.

文摘：全面综述植物酰脲降解代谢酶的研究现况，概述植物尿囊素酶的分布、性质、结构，以及调节控制，并简介植物尿囊酸酰胺水解酶和脲基乙醇酸酰胺水解酶的研究近况，同时指出待研究的方向。

Two inducible and extracellular chitosanases secreted by Penicillium sp. D-l were purified by ammonium sulfate precipitation as well as gel filtration. The purified enzymes of ChoA and ChoB had molecular masses of 93 and 21 kDa by SDS-PAGE. Both ChoA and ChoB have an acidic pH optimum, which were 4.0. The enzymes were stable over the pH range 3 to 5 for 1 h.

采用(NH_4)_2SO_4盐析法和凝胶过滤层析对Penicillium sp.D-1的壳聚糖酶进行分离提纯，得到两种壳聚糖酶，酶A经变性聚丙烯酰胺凝胶电泳分析，结果显示该酶的分子量约为93 kD；酶B经非变性聚丙烯酰胺凝胶电泳和SDS-PAGE分析，得出分子量约为21 kD。

Enzymes could enhance the deamidation rate of Gln residues. Nutrase was the most effective. The rate of deamidation by Nutrase after 6 hours at the given conditions were up to 40.85 percent, followed by alkaline enzyme and papain. Prozyme A, flavourzyme, pepsin had no obvious effect.

蛋白酶具有明显的水解酰胺基的作用，以中性蛋白酶为最，反应6小时，脱酰胺率高达40.85％，碱性蛋白酶和木瓜酶次之，复合酶A、风味酶和胃蛋白酶的作用较小。

The gene of transglutaminase was amplified from the chromosome of Bacillus subtilis DB403 by PCR and translationally fused with thioredoxin gene in the plasmid pET32a to achieve the expression vector pET32-BTGase.

利用PCR技术，从枯草杆菌DB403染色体上扩增出谷氨酰胺转胺酶基因，将其克隆到大肠杆菌载体pET32a中，成功构建谷氨酰胺转胺酶表达载体pET32-BTGase，并转化大肠杆菌BL21 (DE3)。

Further, the method for producing acrylamide of the present invention is characterized by hydrating acrylonitrile having a concentration of acrolein of 1 ppm or less by a microbial cell containing a nitrile hydratase or a processed product of the microbial cell in an aqueous medium.; Furthermore, the method for producing an acrylamide-based polymer of the present invention is characterized by homopolymerizing the acrylamide or by copolymerizing the acrylamide and at least one unsaturated monomer copolymerizable with the acrylamide.

本发明的制备丙烯酰胺的方法的特征在于，用含有腈水合酶的微生物的菌体或该菌体处理物使丙烯醛浓度为1ppm以下的丙烯腈在水性介质中进行水合反应。本发明的制备丙烯酰胺类聚合物的方法的特征在于均聚所述丙烯酰胺，或共聚所述丙烯酰胺和能与丙烯酰胺共聚的至少一种不饱和单体。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。