酰胺酶

On the other hand, the amidase activity and esterase activity of papain were determined at different temperatures.

一方面，通过比较常温溶液体系、低温溶液体系以及低温固液体系中的肽合成产率，发现低温和固液共存是动力学控制肽键合成的两个有利因素：另一方面，通过对木瓜蛋白酶催化水解动力学的研究，分别测定了该酶在不同温度下的酯酶活性和酰胺酶活性。

The different effects of water-miscible organic solvents on the esterase and amidase activities of trypsin have been known for some time.

本文首次报道在单相共溶剂体系通过溶剂的疏水作用对木瓜蛋白酶的酰胺酶活性和酯酶活性进行调控。

Acrylonitrile or methacrylonitrile is hydrolyzed in a suitable aqueous reaction mixture by an enzyme catalyst characterised by a nitrile hydratase and amidase activity of Comamonas testosteroni 5-MGAM-4D, producing the corresponding acid.

在合适的含水反应混合物中通过催化剂使丙烯腈或甲基丙烯腈进行水解生产对应的酸，催化剂的特征在于具有睾丸酮假单胞菌5-MGAM-4D的腈水合酶和酰胺酶活性。

Results The percentage of produced metallo-β-lactamase, lossed outer membrane OprinD2 and active efflux system in P A resistance to carbapenem are 29.8%, 31.9% and 31.9%, produced other β-lactamase is 6.4%, Conclusion Metallo-β-lactamase production is one of the main mechanisms in PA resistance to carbapinem, the result of correct detection in laboratory can not only promote the rational application of antimicrobial agents but also prevent the spread of bacterial resistance

结果 产金属酶、D2缺失、外排泵出分别占29.8%、31.9%、31.9%，产其他β-内酰胺酶占6.4%。结论产金属酶是PA耐碳青酶烯类抗菌药物最主要机制之一，准确的实验室检测不仅可以帮助临床合理选用抗菌药物并可减少细菌耐药性的传播。

Methods: We collected 121 of clinical isolates of Enterobacter, including 87 isolates Enterobacter cloacae, 34 isolates Enterobacter aerogenes, all of the isolates were detected by cefoxitin three-dimension test, drug resistance phenotypes estimation, cloxacillin disc synergy test and cloxacillin-potentiated disc diffusion test respectively to find high-level AmpC β-lactamase isolates, drug resistance phenotypes estimation were also used to detected high induction producing AmpC β-lactamases isolates, meanwhile, we took three-dimension test as standard method, and compared to other methods, then calculated the coincidence rates, sensitivities and specificities of them.

采用头孢西丁三维实验、纸片耐药表型推断法、邻氯西林纸片协同法、双纸片邻氯西林增效试验分别检测高产AmpCβ-内酰胺酶株，获得高产AmpC酶在肠杆菌属细菌中的表达状况。采用纸片耐药表型推断法检测诱导和高产AmpC酶，同时以头孢西丁三维实验为标准，其他三种方法与之相比较，分析它们检测高产AmpC酶的符合率、灵敏度和特异性。

Objective To estimate the occurrence of derepressed hyperproduction AmpC β-lactamases,extended-spectrum β-lactamasesand dual enzyme expression ,as well as drug resistance profile in Enterobacter cloacaeisolates at large hospitals in Wuhan.

目的 了解近两年武汉地区四家教学医院院内感染阴沟肠杆菌耐药现状，以及去阻遏持续高产AmpC酶和超广谱β-内酰胺酶及同时表达这两类酶的发生率。

The former is necessary for equilibrium-controlled synthesis of peptide bonds while the latter is necessary for kinetically controlled synthesis of peptide bonds.

酰胺酶活性起水解或合成肽键的作用，因而对平衡控制的肽键合成是必需的；酯酶活性起转移酰基的作用，因而对动力学控制的肽键合成是必需的。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

Coil and B. subtilis using the β-lactamase distribution assay. The results showed that the β-lactamase activities of E.coli are mainly in periplasm while the enzyme produced by B.

内酰胺酶活力测定表明，大肠杆菌产生的酶主要积累在周质空间，而枯草芽孢杆菌产生的酶主要分泌在胞外。

The chromosomally located cephalosporinase and carbapenem-hydrolyzing beta-lactamase NmcA were inducible by cefoxitin and meropenem in E. cloacae NOR-1, and both were stably overproduced in the ceftazidime-resistant mutant.

在E阴沟杆菌NOR-1中，位于头孢菌素酶和氨基甲酰水解的β-内酰胺酶NmcA中的染色体可以被头孢西丁和美罗培南诱发，而且两者在头孢三嗪噻肟抗体突变体中都是稳定的大量产生的。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。