酰胺酶

Bacillus cereus;amidase;acrylamide;purification

蜡状芽孢杆菌；酰胺酶；丙烯酰胺；纯化

High expression of cellular asparagine synthetase is a causative factor for the resistance of leukemic cell to Lasparaginase therapy.

细胞内门冬酰胺合成酶（asparagines synthetase， AS）的表达水平与白血病细胞对左旋门冬酰胺酶耐药有关。

Understand the therapeutic applications of β-lactam antibiotics and β-lactamase inhibitors.

了解β-内酰胺类、β-内酰胺酶抑制剂的临床应用。

We also observed the interaction between outer membrane permeability rate,β-lactamase and clinical effects of 5β-lactam antibiotics.

而且，进一步观察外膜通透率及β-内酰胺酶与5种β-内酰胺类抗生素临床疗效的关系。

"Leukemic cells that resist asparaginase and survive in this protective niche of the bone marrow might be the reason that leukemia recurs in some children who have been treated with this drug," said Dario Campana, MD, PhD, Oncology and Pathology.

Dario Campana，肿瘤学和病理学的医学博士和理学博士，说：&白血病细胞抵抗天冬酰胺酶的效果，从而在这种保护性的小生境中存活下来可能是一些接受了天冬酰胺酶治疗的儿童白血病复发的原因。&

The pathogenic bacterium of prepartum and intrapartum infectious pneumonia mostly were G- bacilli,the pathogenic bacterium of postpartum infectious pneumonia mostly were G- bacilli and staphylococcus aureus.6.The drug resistant rates of G- bacteria to penicillins,cephalosporins,and monocyclicβ-lactam antibiotic were high,However,their compound of addingβ-lactamase inhibitor agents was relatively low resistance,and all G- bacteria were sensitive to carbapenem antibiotics.

产前及产时感染性肺炎的病原菌以G-杆菌多见，而产后感染性肺炎的病原菌主要为G-杆菌和金葡菌。6.G-菌对青霉素类、头孢菌素类及单环类的β内酰胺类抗生素耐药率高，但对其添加β内酰胺酶抑制剂的复合制剂耐药率较低，对碳青霉烯类抗生素均敏感；金葡菌对苯唑西林耐药率低，对万古霉素均敏感。7。

Results After screening, 59 subtracted library clones were isolated which were specific for strain VIB72, and the DNA sequences of these clones were determined. Seventeen fragments showed high homology to the genes of known functions in other bacteria. This includes soluble lytic murein transglycosylase, mobilization protein, transposase (IS66), resistance-related protein (metallo-beta-lactamase and acetyltransferase family), toxin protein (DT-201 and alveicin A immunity protein), ATP-dependent endonuclease of OLD family like protein, SocE and GTP-binding protein HflX (high frequency of lysogenization).

通过对差减文库筛选，分离到59个对菌株VIB72的克隆，并对这些克隆的DNA序列进行了测定。17个基因片断与其它细菌的已知功能的基因有较高的同源性，其中包括可溶性溶胞壁质转糖基酶、转移蛋白MobA和MobC、转座子IS66、抑制相关蛋白（金属β-内酰胺酶和乙酰转移酶家族）、毒素蛋白（DT-201和alveicin A免疫蛋白）、与OLD 家族相似的ATP依赖性核酸内切酶以及SocE 和GTP结合蛋白HflX。

Histological observation showed that degeneration,necrosis of liver cells,edema,extravasated blood,erosion and degranulation of the mast cells in intestine mucosa.

肠谷氨酰胺酶活性降低，肠粘膜对谷氨酰胺利用减少，肠粘膜通透性增加。组织细胞学显示，TAA组动物肝细胞发生严重变性坏死；肠粘膜淤血、水肿、糜烂，肠肥大细胞脱颗粒。

HGF is 2,000 nanograms per ml of a micro-dilution recombinant hybrid Biotropin "The real thing"+ amino acid-polypeptide precursors, Arginine pyroglutemate, Lysine HCL, Glycine and L-Glutamine.

HGF微型稀释液每毫升约含2000毫微克生长因子，稀释液的成分有&真正的生长因子&+氨基酸肽前体，精氨酸酰胺酶，赖氨酸盐酸，甘氨酸和L -谷氨酰胺。

When the platform of screening β-lactamse inhibitor is constructed,it can not only be used to find new compounds for treating bacteria which resist β-lactam antibiotics, but also be used to screen inhibitors of hydrolase or modifying enzyme which is related to drug resistent bateria.

通过建立β-内酰胺酶抑制剂筛选的平台，不仅为寻找治疗β-内酰胺类抗生素耐药菌开辟一条新途径，并且这一新方法也适用于筛选与细菌耐药有关的其它一些由细菌产生的水解或修饰酶的抑制剂。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。