酰胺酶

However, alkylating agents, such as cyclophosphamide, and the topoisomerase II inhibitors, doxorubicin and epirubicin, are associated with two types of cytogenetically distinct leukemias after adjuvant chemotherapy.

然而，烷化剂如环磷酰胺、拓扑异构酶II抑制剂、阿霉素、表阿霉素都与辅助化疗后的两种细胞遗传学不同的白血病的发生相关。

Ingredients: Arbutin 2%, Electrodialyzed Deep Sea Water, Mink Oil, Emu Oil, Borage Seed Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Centella asiatica L.

主要成分：含熊果苷2%，海洋深层水，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，玫瑰果油，突厥蔷薇，蜂胶，积雪草，黄芩，甘草，金银花瓢虫，银杏叶，人参，洋甘菊，葛根等提取物

Ingredients: Electrodialyzed Deep Sea Water, Disodium Laureth sulfosuccinate, Coco-Betaine, Mink Oil, Emu Oil, Jojoba Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Rose Hip Oil, Borage Seed Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Morus alba L.

主要成分：含海洋深层水， Disodium Laureth sulfosuccinate，可可甜菜碱，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，玫瑰果油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，弹性蛋白水解，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，突厥蔷薇，蜂胶，薄荷，芝麻，何首乌，银杏叶，枸杞，荨麻腿子属，洋甘菊，葛根等提取物

Hebei University of Technology, TianJin 300130, China Abstract: Chromobacerium pathogens S-4 with herbicidal activity was screened from rhizosphere of Digitaria sanguinalis using the glutamine synthetase inhibitor. The condition of S-4 in liquid fermentation was studied.

河北工业大学化工学院，天津 300130 摘要：采用谷氨酰胺合成酶抑制剂模型从马唐根际土壤中分离出一株马唐致病菌S-4，经鉴定为色杆菌属，对马唐抑制效果较好。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

The first part reports some new reactions of BNAH, especially the reductive cyclizations of some activated ethylenic compounds to five-membered carbocycles and a sevenmembered enolic ether ring, both of which may be useful in related areas.

详细论文的研究对象是重要的辅酶NADH的化学模型1-苄基-1，4-二氢烟酰胺。论文包括一个对近期该领域进展的综述和三部分工作。

The glutamate oxaloacetate transaminase isozyme of thirty-three accessions of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum including seven species of the big-achene group and one species of the small-achene group were studied by means of polyacrylamide gel eletrophoresis.

用聚丙烯酰胺凝胶电泳技术时荞麦属8个种（含大粒组7个种和小粒组1个种）33份荞麦资源的谷草转氨酶同工酶进行了研究。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

Methods Three kinds of special sequence primers for polymerase chain reaction with regard to the mutation styles (CGT、GTT、GAT) at codon 12 of K-ras were used to study the human pancreatic cancer cell line Patu 8988.The amplification products were studied with 12% polyacrylamine gel eletrophoresis to detect the style of point mutation.

针对K-ras基因第12位密码子点突变方式(CGT、GTT、GAT)设计顺序特异性引物，对人胰腺癌细胞株Patu 8988进行聚合酶链反应，扩增产物经12%非变性聚丙烯酰胺凝胶电泳后判断该细胞株有无K-ras基因点突变及其突变方式。

This research concerns about the isozymes analysis of parasitic sarcocystis hirsuta from water buffalo and cattle by means of isoelectric focusing electrophoresis to explore the host specificity of sarcocystis species.

采用聚丙烯酰胺垂直平板梯度凝胶电泳的方法，对寄生于黄牛和水牛的毛状肉孢子虫进行同工酶比较研究，探讨肉孢子虫的宿主特异性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。