酰胺酶

The α-AE_(75) cDNA was fused downstream of melC1 signal peptide encoding sequence in correct reading frame so that α-AE_(75) will be expressed secretively.

用这两个表达质粒分别转化S.lividans得到能分泌表达α-酰胺化酶的工程菌S.lividans [pIJmel／AE680]和S.lividans[pMS-mel／AE680]。

The effects of the immobilization conditions, the structure of spherule, the mechanical strength, elasticity, stability, and the activity of the bacterial enzyme were investigated, analyzed and determined the immobilization conditions.

探讨了固定化条件对固定化小球结构、机械强度、弹性、稳定性和培养后菌体的谷氨酰胺合成酶的活性情况的影响，分析确定最佳的固定化条件。

Subtle changes, such as oxidative damage-induced loss of glutamine synthetase activity, may be a key event in stroke-induced brain injury.

一些细微的变化，比如氧化损伤诱导的谷氨酰胺合成酶活力丧失，可能是中风引起的脑损伤的关键环节。

Consequently,the cost of glutamine synthetase used inindustry was reduced.

业用谷氨酰胺合成酶的成本。

The pI of these components gradually decreased f rom pH 4.0 according to electrophoresis mobility from higher to lower on PAGE. The molecular weights were in the range of 22～34 ku. 6.5 and 7 w ere glycoproteins proved by staining with the shiff reagent and thymol/sulfuric acid. The fibrinolytic activity of 7 was highest as determined usi ng chromzym UK and chromzym PL as specific substrates.

这些组分的等电点按照它们在聚丙烯酰胺凝胶电泳图谱上的顺序从4.0开始依次降低；SDS-PAGE证明，除3、4外，其余组分均只含一种多肽链，分子质量在22～34ku之间；用shiff试剂和酚-硫酸染色，显示1、2、6.5和7是糖蛋白，其中7的糖含量最高；以BAEE、Chromozym UK和Chromozym PL为底物测定，7的纤溶酶活性最高。

It was discovered that proteases, lipases and abzymes can be used for the regioselective acylation of sucrose.

酶催化合成蔗糖酯通常在N，N-二甲基甲酰胺，二甲基亚砜和吡啶等有毒溶剂中进行。

Imidase ; Gene cloning ; Sequence analysis ; Gene expression ; Alcaligenes eutrophus

酰亚胺酶；基因克隆；序列分析；基因表达；真养产碱杆菌

Methods TGGP was purified from Balanophora japonica Makino by n-hexane and ethyl acetate extraction and column chromatography.

用萃取和柱层析方法分离提纯TGGP，用酶联免疫测定法、天然聚丙烯酰胺凝胶电泳、分子排阻高效液相色谱法检测其抑制HIV gp41六螺旋束结构形成的作用，用细胞－细胞融合方法检测TGGP抑制HIV进入靶细胞的活性。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

In order to further understand combining characteristic of cyclohexyl methylphosphonofluoridate and isopropyl methylphosphonofluoridate and contribute more scientific data for its medical protection, the potency of inhibition of acetylcholinesterase activity induced by GF, GF+GB and the effects of reactivation by oximes pyramidoxime monohydrate(HI-6) and pyridine-2-aldoxime methylchloride(2-PAM-Cl) in rat brain were investigated with DTNB microanalysis in this study.

为进一步了解甲氟膦酸环己酯与甲氟膦酸异丙酯的联合特性，为制订医学防护措施提供更多的理论依据，用微量DTNB法对GF，GB和GF与GB混合对大鼠脑乙酰胆碱酯酶的抑制及肟类重活化剂酰胺磷定（HI-6）和氯磷定（2-PAM-Cl）的重活化作用进行了研究。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。