酰胺酶

In this experiment, we made a comparison and analysis of peroxidase total activity and isozymes of Paulownia trees with and without witch's broom by using spectrophotometer and incontinuous polyacrylamide gel electrophoretic methods.

分别采用分光光度法和不连续聚丙烯酰胺凝胶盘状电泳法，比较分析了感病与未感病泡桐树木的过氧化物酶总活性与同工酶的变化。

Using polyacrylamide gel electrophoresis, the peroxidase isoenzyme of three different resistant varieties of apple seedlings inoculated with Marssonina coronaria was studied.

应用聚丙烯酰胺凝胶电泳，测定抗性不同的3个苹果品种过氧化物酶同工酶在接种褐斑病菌后的变化规律。

The detection rate of DHA-l is much higher than that in other regions; Pa isolated with DHA-1 gene showed powerful activities with resistance to cephalosporin and monocyclic lactam antibiotics. The prevalence of Pa in the same or different departments should be noted seriously.

淮北矿工总医院DHA-1型质粒AmpC酶检出率远高于国内其他地区；产DHA-1型质粒AmpC酶菌株对头孢菌素及单环酰胺类抗生素耐药严重，在同一科室或不同科室间的传播应引起高度重视。

The Esterase, Catalase, PPO isoenzyme and soluble mycoprotein patterns of 43 isolates were analyzed by polyacrylamide gel electrophoresis.

利用聚丙烯酰胺凝胶电泳技术对43个新月弯孢分离物进行了酯酶等三种同工酶及可溶性蛋白的分析。

With the vertical flat electrophoresis of polyacrylamide gel, 12 Pholiota adipose strains from different regions were compared and analyzed in esterase isozyme character and the genetic differences among strains were analyzed with the cluster analysis software.

方法］利用垂直板聚丙烯酰胺凝胶电泳，对来源于不同地区的12个黄伞菌株进行酯酶同工酶比较和分析，并通过聚类分析软件对各菌株间的遗传差异性进行分析。

Cloning of cytosolic (GS1) cDNA and chloroplast glutamine synthetase (CS2) cDNA from Pisum satium was carried out previously in our laboratory.

谷氨酰胺合成酶(Glutamine synthetase， GS， E。 C。 6。 3。 1。 2)是植物氨同化过程中的关键酶，对植物的氮素吸收和代谢起着至关重要的作用。

Cloning of cytosolic (GS1) cDNA and chloroplast glutamine synthetase (GS2) cDNA from Pisum satium was carried out previously in our laboratory.

谷氨酰胺合成酶(Glutamine synthetase，GS，E.C.6.3.1.2)是植物氨同化过程中的关键酶，对植物的氮素吸收和代谢起着至关重要的作用。

objectiveto establish a peroxidase isoenzyme method for identification of rugosa rose varieties in shandong province.methodsthe polyacrylamide gel electrophoresis method was applied.

目的建立山东不同品种玫瑰花药材的过氧化物酶同工酶鉴别方法。方法聚丙烯酰胺凝胶电泳法。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

Results Patients who had at least one abnormal result of liver function test accounted for 70.99% in untreated group and 61.07% in treated group. The most common abnormal hepatic function parameter in patients with thyrotoxic hepatic lesion was increased alkaline phosphate enzyme, which was 57.3% in untreated group and 51.0% in treated group. Abnormalities of alanine transaminase, aspartate transaminase, gamma-glutamyl tyanspeptidase and total bilirubin were 28.2%(37/131), 18.3%(24/131), 10.7%(14/131) and 21.4%(28/131) in untreated group and 21.5%(32/149), 15.4%(23/148), 4.7%(7/149) and 20.1%(30/149) in treated group, respectively. The possibility of thyrotoxic hepatic dysfunction was correlated with the patients' age and FT3 and FT4 levels, but not with the genesis and course of disease, sex, family history of hyperthyroidism, or levels of TGAb and TMAb. Conclusion Abnormal results of liver function test are commom in patients with hyperthyroidism.

结果 在未治组甲亢性肝功能损害的发生率为70.99%，经治组为61.07%；甲亢性肝功能损害最常见的异常指标是碱性磷酸酶增高，未治组、经治组分别为57.3%、51.0%；血清谷丙转氨酶异常在两组的发生率分别为28.2%、21.5%；血清谷草转氨酶异常在两组的发生率分别为18.3%、15.4%；总胆红素异常在两组的发生率分别为21.4%、20.1%；谷酰胺转肽酶异常的发生率未治组和经治组分别为10.7%、4.7%；甲亢性肝功能损害的发生与病程、甲状腺疾病家族史、性别、TGAb水平、TMAb水平无关，与年龄、甲状腺激素水平有关。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。