酰胺酶

Effects of hydroxylamine addition on the accumulation of MTG by Streptomyceshygroscopicus WSH03-13 were investigated.

研究了添加羟胺对吸水链霉菌WSH03-13合成谷氨酰胺转胺酶的影响。

The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示：先证者尿检呈强阳性；其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变，为半合子突变，而其母、其姐为杂合突变；正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变；不同物种氨基酸序列的同源性比对显示： c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守；酶活性测定的结果显示：先证者的IDS酶活性仅为2.3 nmol/4 h/mL，大大低于正常值，而其父的为641.9 nmol/4 h/mL，其母的血浆酶活性为95.8 nmol/ 4h/mL，其姐的为103.2 nmol/4 h/mL。

Autolytic speed of lactic acid bacteria which is determined by the autolytic activity not only affect age velocity of cheese,but also influence the bacteria count of high density culture starter.

通过聚丙烯酰胺凝胶-细胞电泳法对乳酸菌杆LB-3细胞培养液中自溶酶的活性、细胞死亡与自溶酶的关系及温度对自溶酶活性的影响进行了研究。

Compared with prozyme A, alkali enzyme was a powerful enzyme for the hydrolysis of corn protein, but it could result deamidation, that was another reason for selecting prozyme A as the first enzyme.

与复合酶A相比，碱性蛋白酶也能较好地作用于黄粉蛋白，但它对酰胺基有较强的水解作用，这是选择复合酶A作为第一次水解用酶的又一个主要原因。

Deamidation of the milk protein produced the spicy or ammonia-like flavor.Reduction and heat treatment had no obvious effect on the enzyme reaction and the flavor of the product.The order of different enzymes could influence the flavor effectively.In addition,the taste of the hydrolysate can not be improved by adding condiment.

结果表明，脱酰胺反应会使产品具有氨味和辛辣味；还原处理和加热处理，对脱脂乳粉和乳清浓缩蛋白的酶解速度和水解液风味都无明显影响；不同的酶解顺序对乳蛋白水解液的风味影响显著；用风味剂不能有效地掩盖酶解液的不良风味。

The results indicate that Ca(superscript 2+) and GA3, single or combined application treatments, promote cucumber seed germination treated with P. harmala alkaloid, increase germination rate, germination vigor and index; as well as α-amylase, protease, lipase, isocitratelyase, nicotinamide adenine dinucleotide kinase and Ca(superscript 2+)-ATPase activities; and GA and CaM content in cucumber seeds; but decrease abscisic acid content. The synergistic effects of combined Ca(superscript 2+) and GA3 on increasing GA and decreasing ABA are oberseved compared with Ca(superscript 2+) treatment.

结果表明，Ca(上标 2+)和GA3单一及复合处理可促进骆驼蓬生物碱处理的黄瓜种子的萌发，使黄瓜萌发率、发芽指数和活力指数提高，萌发种子的α-淀粉酶、蛋白酶、脂肪酶和异柠檬酸裂解酶活性及赤霉素和钙调素含量增加，烟酰胺腺嘌呤二核苷酸激酶和Ca(上标 2+)-ATP酶活性增强，脱落酸含量降低；且Ca(上标 2+)和GA3复合处理对GA含量的提高和ABA含量的降低与Ca(上标 2+)单一处理比较呈显著的加成作用。

The changes of peroxidase and phenylalanine ammonia lyase in several jujube cultivars inoculated with phytoplasmas were analyzed by using spectrophotometer and incontinuous polyacrymide electrophoretic methods.

采用分光光度计和不连续聚丙烯酰胺凝胶电泳，比较分析了不同枣树品种及株系接入病原植原体后，其过氧化物酶及同工酶和苯丙氨酸解氨酶的变化。

SOD, ATPase, MDH and ME isoenzymes in gill, digestive gland, foot, adductor muscle and mantle of Mytilus edulis were tested and analyzed using polyacrylamide gel electrophoresis.

采用聚丙烯酰胺垂直电泳法对紫贻贝 Mytilus edulis 的鳃、消化腺、外套膜、足、闭壳肌等5种组织的超氧化物歧化酶、三磷酸腺苷酶、苹果酸脱氢酶、苹果酸酶进行了检测分析。

The activities of nitrate reductase, glutamate dehydrogenase and glutamine synthetase in water spanich leaves were enhanced, the contents of total nitrogen and protein increased, but the content of free amino acid decreased. Compared to the control, low and medium amount of quinhydrone made significant difference respectively, but high amount had not notable effects.

醌氢醌使蕹菜叶片硝酸还原酶活性、谷氨酸脱氢酶活性、谷酰胺合成酶活性、总氮含量、蛋白质含量提高，游离氨基酸含量降低；与对照相比，低、中量醌氢醌处理差异显著，高量醌氢醌差异不显著。

The results show that during the development of the oriental tobacco leaves, the activity of acid invertase was decreased; the changes of GS reached its peak in the 20dans in Samsun, but in the 30dans in Basma and Canik ;the activity of PAL was decreased in the 30dans; The content of total nitrogen was decreased with the mature leaves, but nicotine、total carbon and soluble total sugar were increased. Different varieties, carbon and nitrogen metabolism key enzyme activity and the content of metabolites were different.

结果表明，在香料烟叶片发育过程中，酸性转化酶的活性均呈下降趋势；谷氨酰胺合成酶的活性Samsun品种在20d叶龄时最高，而Basma和Canik在30d叶龄时最高；苯丙氨酸解氨酶的活性均表现为30d时最低；随着叶片成熟总氮含量降低，烟碱含量升高，总碳、可溶性总糖随着叶片成熟其含量呈升高趋势；品种不同，碳氮代谢关键酶的活性及代谢产物的含量变化均有很大差异。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。