酰胺酶

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

PAGE was used to analyze and compare the variation of composition and content of four isoenzymes , esterase, acid phosphatase, malate dehydrogenase and lactate dehydrogenase, of Paramecium bursaria which cultured in light and dark .

作者应用聚丙烯酰胺凝胶电泳技术分析比较了光培养绿草履虫和暗培养绿草履虫中酯酶、酸性磷酸酶、苹果酸脱氢酶和乳酸脱氢酶四种同工酶的组分及含量的变化。

The zymogramatic variations of esterase isoenzyme and superoxide dismutase isoenzyme of 20 sample materials, including 5 species, 2 varieties, 1 multiploid of Isatis L. and 1 outgroup species, Coronopus didymus J. E. Smith, were analyzed with technique of polyacrylamide gel electrophoresis.

采用聚丙烯酰胺凝胶电泳，比较了菘蓝属5种2变种1多倍体品种及1外类群种共计20个样本的酯酶同工酶和超氧化物歧化酶同工酶的酶谱差异，并运用数量分类学的原理和方法对酶谱数据进行了聚类分析。

The researchs on isozymes indicated that some varieties of isozyme loci present during the embryo development. There are 7 esterase loci and 5 acid phosphatase loci during the periods between nucellar filament and early globular embryo; both of Est and Acp appeared the most diversity in the stage of 8-nucleate embryo sac.

用聚丙烯酰胺凝胶电泳的方法研究鹤顶兰从指状突起出现到早期球形胚形成的全过程中酯酶、酸性磷酸酶的变化发现：这一过程中酯酶表达有7个同工酶位点，酸性磷酸酶有5个同工酶位点，两者均在八核胚囊时期变化最显著。

Seven isozymes were analyzed by means of horizontal sliceable starch gel electrophoresis.

采用水平切片淀粉凝胶电泳检测了七种同工酶的酶谱图式，从中筛选出酯酶同工酶进一步作聚丙烯酰胺凝胶电泳分析。

Base on the analysis, organophosphate inducible system has been established by treatment of the swimming seedling and drenching roots with organophosphate solution. This inducible system consists of model plant Arabidopsis thaliana and methamidophos, folimat or methyl parathion which are the representative types of highly toxical OPs. Meanwhile, the responses of the esterase isozymes and the total soluble proteins to OPs in Arabidopsis thaliana have been detected by Native-PAGE and SDS-PAGE.

在此基础上，采用模式植物拟南芥和甲胺磷、氧化乐果和甲基对硫磷三种极具代表性的高毒高残留有机磷农药，通过水培和灌根的方法，建立了有机磷诱导系统，并应用变性与非变性聚丙烯酰胺凝胶电泳技术，研究了拟南芥酯酶同功酶和可溶性总蛋白对有机磷胁迫的响应。

Objective To study the effects of berbamine and batiolun on LDH metabolizability in the process of increasing leukocyte.Methods Enzyme biochemical techniques is used to study the effect of berbamine and batiolˉun in the process of inceasing leukocyte and also to study the effect of LDH metabolizability.

目的 研究小檗胺和鲨肝醇升高白细胞作用及对细胞内LDH的代谢的影响方法采用酶细胞化学方法，对两药物在治疗由免疫抑制剂环磷酰胺所致的白细胞减少症时，对乳酸脱氢酶活性的影响进行了比较观察。

(Times New Roman小四号,加粗,顶格)Using the vertical polyacrylamide gel electophoresis, the differentiation of peroxide, catalase and esterase isoenzyme from roots, caudexes and leaves of three wild buckwheat were analyzed.

(宋体小四号，加粗，顶格)采用垂直板聚丙烯酰胺凝胶电泳对三份野生荞麦根，茎和叶的过氧化物酶，过氧化氢酶和酯酶的同工酶进行了酶谱分析。

Effect of glucose oxidase and transglutaminase on theological properties of doughs and qualities of flour product s.

该文综述葡萄糖氧化酶和谷氨酰胺转胺酶的特性、作用机理，及其对面团流变学特性和面制品品质影响。

The electrophoretograms of isozymes from five different tissues （foot, mantle, adductor muscle, gill, liver） of Mercenaria mercenaria Linnaeus were analyzed, by vertical polyactylamide gel electrophoresis.

采用垂直板聚丙烯酰胺凝胶电泳技术，对硬壳蛤5种组织（足、外套膜、闭壳肌、鳃和肝）的酯酶、苹果酸脱氢酶和乳酸脱氢酶同工酶进行了比较研究，并对酶谱表型及位点表达进行了分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。