酰胺酶

An amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using sonication, anion-exchange chromatography, phenyl-sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 ( Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子色谱、Phenyl-Sepharose疏水色谱等步骤获得了凝胶电泳均一的酰胺酶。

A amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using of the sonication, anion-exchange chromatography, Pheny1-Sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 (Bacillus cereus ZJB-07112)发酵生产酰胺酶，经超声波细胞破碎、High Q阴离子层析、Phenyl-Sepharose疏水层析等步骤获得了凝胶电泳均一的酰胺酶。

OBJECTIVE: To study the cellular activity of asparagine synthetase in different types of childhood acute lymphoblastic leukemia.

目的：不同类型急性淋巴细胞性白血病对左旋门冬酰胺酶敏感度不完全相同，由于LAsp活性水平与细胞内门冬酰胺合成酶活性负相关，因此研究不同类型ALL患儿白血病细胞内门冬酰胺合成酶活性水平分布情况，有助于临床治疗中合理使用LAsp。

Objective To study the effects of different doses of manganese on activities of GS and PAG in the rat striatum and the activities of SDH and Na^＋-K^＋-ATPase in pallium in order to explore the cell molecular mechanism of manganese-induced exeitotoxicity.

目的研究不同剂量锰对大鼠纹状体谷氨酰胺合成酶、谷氨酰胺酶和脑皮质琥珀酸脱氢酶、钠钾三磷酸腺苷酶（Na^＋-K^＋-ATPase）活性的影响，以探讨锰的兴奋性毒性的细胞分子学机制。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Methods Ampicillin inhibitionof E. coli Top10 pcs+ was tested at first, and then b-lactamase activity in periplasm was examined.

然后再使用抗生素抗性分析、b-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的b-内酰胺酶从细胞质到细胞间质的分泌情况。

85 And 119486/79. They are a novel type of antibiotic having both the wide antibacterial spectrum and high safety of penicillin and cephem antibiotics belonging to beta -lactam antibiotics, as well as the potent antibacterial activity and high beta -lactamase stability of carbapenem antibiotics.Sodium-(5R, 6S)-6-[-1-hydroxyethyl]-7-oxo-3- [-2-tetrahydrofuryl]-4-thia-1-azabicyclo [3.2.0] hept-2-ene-2-carboxylate 5/2 hydrate (faropenem sodium, hereinafter referred to as compound 1) is currently used as an oral drug for various infectious diseases and is reported to show potent antibacterial activity against not only methicillin-sensitive Staphylococcus aureus, Streptococcus pyrogenes and Streptococcus pneumoniae but also gram-positive bacteria for which conventional beta -lactam drugs have proved ineffective such as penicillin-resistant pneumococci, oral staphylococci and enterococci, also showing a wide antibacterial spectrum covering gram-negative bacteria such as Haemophilus influenzae and anaerobic bacteria such as the genus Bacteroides, which activity is due to its novel skeleton penem ring (Kagaku Ryoho no Ryoiki The Field of Chemotherapy, Vol.

他们是一种新型的抗生素都具有广泛的抗菌谱和高度的安全青霉素和cephem抗生素，属于β-内酰胺类抗生素，以及作为强大的抗菌活性和高β-内酰胺酶稳定的碳青霉烯类antibiotics.sodium -（ 5 R ， 6 S ）-6 -[ - 1 -羟乙基] - 7 -氧- 3 - [ - 2 - t etrahydrofuryl] - 4 -硫杂- 1 -氮杂双环[ 3 。2.0]庚- 2 -节能- 2 -羧酸5 / 2水合物（法罗培南钠，以下简称为化合物1 ）是目前用来作为口服药物，为各种传染病和报道，以显示强大的抗菌活性，不仅对甲氧敏感的金黄色葡萄球菌，链球菌pyrogenes和肺炎链球菌，但也克阳性菌为常规β-内酰胺类药物已证明无效，如青霉素耐药pneumococci ，口腔葡萄球菌和肠球菌，也显示出广泛的抗菌谱，包括革兰阴性菌如流感嗜血杆菌和厌氧菌如属杆菌，这活动是由于其新颖的骨架青霉烯环（（化学ryoho没有ryoiki领域的化疗），第13卷，第10号，第74-80 ， 1997年）。

Objective To investigate extended-spectrumβ-lactamasesand AmpCβ-lactamases and metalloβ-lactamasesproducing by gram-negative bacilli in burns.Mehtods Adopitng double-disk synergy test,modified three-dimensional extract test and the enzyme inhibitor of EDTA-Na2to detect ESBLs and AmpC and mBLA producing strains.

目的 了解烧伤感染主要革兰阴性杆菌产超广谱β-内酰胺酶、AmpC酶和金属β-内酰胺酶及其对常用抗生素耐药性情况，指导烧伤医院感染合理用药。

Objective To investigate extended-spectrumβ-lactamasesand AmpCβ-lactamases and metalloβ-lactamasesproducing by gram-negative bacilli in burns.Mehtods Adopitng double-disk synergy test,modified three-dimensional extract test and the enzyme inhibitor of EDTA-Na2to detect ESBLs and AmpC and mBLA producing strains.Using K-B test to perform the susceptibility testing.Results Among348strains of gram negative bacilli,ESBLs,AmpCβ-lactamases,ESBLs combined with ApmCβ-lactamases and mBLA produc-ing strains were found in122,47,9and20strains,the incidence being35.1%,13.5%,2.6%and5.7%respec-tively.

目的 了解烧伤感染主要革兰阴性杆菌产超广谱β-内酰胺酶、AmpC酶和金属β-内酰胺酶及其对常用抗生素耐药性情况，指导烧伤医院感染合理用药方法采用双纸片协同试验检测产ESBLs株，头孢西丁三维试验检测产AmpC酶株，头孢曲松三维试验检测同时产ESBLs和AmpC酶株，依地酸二钠协同试验检测产mBLA株，K-B法进行G -杆菌药敏试验。

The therapeutic problems posed by class D beta-lactamases, a family of serine enzymes that hydrolyse beta-lactam antibiotics following an acylation-deacylation mechanism, are increased by the very low level of sensitivity of these enzymes to beta-lactamase inhibitors.

丝氨酸酶一个科能水解β-内酰胺抗生素和随后进行酰化和脱酰化机理， D类β-内酰胺酶引起的治疗问题，随着这些对β-内酰胺酶抑制剂的酶的非常低敏感性而不断增加。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。