酰胺化

The α-AE_(75) cDNA was fused downstream of melC1 signal peptide encoding sequence in correct reading frame so that α-AE_(75) will be expressed secretively.

用这两个表达质粒分别转化S.lividans得到能分泌表达α-酰胺化酶的工程菌S.lividans [pIJmel／AE680]和S.lividans[pMS-mel／AE680]。

The key steps involved Friedel-Crafts reaction of o-cresol and the Wittig reaction for construction the skeleton of amine moiety,at the same time, using the synthesis of E,(7S)-7-methoxytetradec-4-enoic acid in chapter 2,and Johnson-Clainsen rearrangement as a key step for construction the fatty acid part,the corresponding acid was prepared in 23%and 50%overall yield,respectively.

随后将手性不饱和脂肪酸和氨片段进行酰胺化反应构筑了malyngamide M的基本骨架，再将Z-烯氯转化为E-烯氯完成了目标分子的全合成，高立体选择性地构筑了末端烯氯结构，为制备其它含有类似烯氯结构的malyngamides探索出行之有效的合成路线。

The amidation of C-terminal is crucial for its full biological activity.

其C末端的酰胺化对它完整的生物活性起重要作用。

The amidation reaction between rapeseed oil and onoethanolamine was studied via orthogonal experiment.

通过正交实验对菜籽油与单乙醇胺的酰胺化反应进行了研究。

Catalytic aziridination and amidation reactions are two important reactions in organic synthesis and useful building blocks for organic synthesis, which make them attractive synthetic targets in their own right.

催化氮杂环丙烷化及酰胺化反应是有机合成中的两类重要反应，是有机合成之砌块，其合成方法是重要的研究课题。

To avoid the hydrolyzation of the expressed product by the cellular proteases and get more stable humanα-defensin it was attempted to express humanα-defensingenes in cyclic or amidation form.

为了避免表达产物水解，得到更稳定的人α-防御素，以环化形式和酰胺化形式表达人α-防御素基因。

The results of the analysis of the pectin extracted show ed that the degree of free and Salted forms, the degree of amide substitution,and the degree of esterification of carboxy groups were 85.1%, 1.1%, and 13.8%,...

我们对蚕粪果胶的分析表明，其羧基的游离及成盐程度、酰胺化程度、酯化程度分别为85.1％、1.1％和13.8％，甲氧基含量、半乳糖醛酸含量分别为1.79％和81.5％；特性粘度为267。

Was synthesized from cyanoacetic acid and diethylamine via DCC coupling under mild conditions.

中间体5 的制备由氰基乙酸在二环己基碳二亚胺作用下经酰胺化一步制得，条件温和，收率较高，避免使用剧毒的氰化钠。

At last,a convergent and general protocol to marine natural product,as demonstrated by the enantioselective total synthesis of malyngamides O,P,Q,R, 5"-epi-Q and 5"-epi-R bearing terminal vinylic chloride structure,was developed in detail.E,(7S)-7-methoxytetradec-4-enoic acid was prepared using the above described Johnson-Clainsen rearrangement,the terminal vinyl chloride was constructed using Wittig reaction as key step,the pyrrolidone derivative was constructed using L-serine as natural chiral pool.Starting from the synthesis of malyngamides O and P to explore the general route,an aldol reaction of methyl acetate with the vinyl chloride part in the presence of LDA gave the skeleton of amine portion,followed by amidation with the fatty acid finished the synthesis of malyngamide P.

先以malyngamides O和P为试点探索通用合成路线，将乙酸甲酯和烯氯部分在LDA存在下进行aldol反应得到氨部分的骨架，再将手性不饱和脂肪酸和氨部分进行酰胺化反应完成了malyngamide P的合成，将P在碱性条件下进行烯醇甲基化反应完成了malyngamide O的全合成。

In our research, through derivatization of stable calix[4]pyrroles, active substituting units were jointed on the calixpyrrolic skelecton. After acylamide and free radical transfer reaction, the preparation of new calix[4]pyrrole-bonded stationary phases were fulfilled. the separation abilities of them to inorganic and organic anions and medicines were exploited and new analytical methods were established. The separation mechanisms were discussed. At the same time, through using calix[4]pyrroles as additives in CZE to influence the electrophoresis of inorganic anions, the recognition mechanisms and influencing factors in supramolecular chemistry were also studied.

本项目以新型杯吡咯大环化合物分子识别化学研究为基础，设计合成了含活泼取代基的官能化杯[4]吡咯化合物，通过酰胺化反应和原位自由基链转移反应制备了新型的杯[4]吡咯色谱固定相，系统研究了具有阴离子识别功能的杯[4]吡咯大环化合物作为离子色谱的分离介质对无机、有机离子型化合物和药物分子的色谱分离性能，建立了新的分析方法，对分离机理进行了探索和验证；同时将杯[4]吡咯化合物用作毛细管电泳流动相添加剂，利用其对无机阴离子电泳行为的影响探讨超分子化学中分子识别的机理和影响因素。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。