酰氨

Gln group received pure Gln through a jejunostomy tube, IN group received indomethacin, G+I group received Gln and indomethacin.

材料与方法：将SD大鼠随机分成四组：谷氨酰胺组（Gln组，n=34）；吲哚美辛组（IN组，n=34）；谷氨酰胺+吲哚美辛组（G+I组，n=34）和SAP组（n=34）。

By the methods of molecular biology, enzymology, chemistry, physics the project was carried out. The genes encoding leucyl- and arginyl-tRNA synthetase , and tRNA and tRNA from Escherichia coli were cloned and overexpressed in E.

以大肠杆菌亮氨酰tRNA合成酶和精氨酰tRNA合成酶为对象用分子生物学、酶学、酶动力学、化学、物理学的方法进行该项研究。

D2 hydantoinase (EC3151212) can change the D type racemates of the racemic D,L252 monosubstituted hydantoin to D2N2 carbamoylase, and the leftover L252 monosubstituted hydantoin due to never reacted with D2 hydantoinase, shown as figure 1, firstly racemizing, thus makes the D,L2 hydantoin completely changes to pure optical D2N2 carbamoylase, and then be changed to D2 amino acid.

海因酶具有底物特异性，D2海因酶(EC 3151212)可转化外消旋的D ，L252单取代海因中的D型消旋体成为D2N2氨甲酰氨基酸，剩余的L252单取代海因由于完全不被 D2海因酶所作用，如图 1所示，先进行消旋，从而使得 D ，L2海因完全转化为光学纯的 D2N2氨甲酰氨基酸，并进一步被转化为D2氨基酸。

Most of the studies have demonstr ated that glutamine serves metabolic roles for macrophage.By glutamiolysis pat hway,glutamine provides ATP for the cellular metabolism,intermediates for the biosyn thesis of parine,pyrimidine and nuclecotides,and nitrogen for the formation of glucosamine,GTP and NAD+.

研究表明谷氨酰胺是单核巨噬细胞主要的代谢底物，通过谷氨酰胺酵解途径，为细胞代谢提供能量；为细胞合成DNA和mRNA提供嘌呤、嘧啶、核苷酸生物合成前体；提供氨基葡萄糖，GTP和NAD+合成的氮前体。

The gene of transglutaminase was amplified from the chromosome of Bacillus subtilis DB403 by PCR and translationally fused with thioredoxin gene in the plasmid pET32a to achieve the expression vector pET32-BTGase.

利用PCR技术，从枯草杆菌DB403染色体上扩增出谷氨酰胺转胺酶基因，将其克隆到大肠杆菌载体pET32a中，成功构建谷氨酰胺转胺酶表达载体pET32-BTGase，并转化大肠杆菌BL21 (DE3)。

Based on the fact that aminoacyl-tRNA synthetase is a bona fide drug target for several microorganisms, including bacteria and fungi, it is plausible that it may also be effective target of Trypanosoma. In this report, the Trypanosoma brucei Leucyl-tRNA synthetase was cloned , expressed and purified to develop an in vitro enzymatic assay system.

根据亮氨酰tRNA合成酶在微生物中可作为药物靶点的事实，在新型抗锥虫药物筛选中，通过对锥虫的亮氨酰tRNA合成酶的克隆、表达和纯化，以及该酶活性测定的优化，建立了该酶抑制物的筛选系统。

RESULTS: A germacrane sesquiterpenoid, incaspitolide D (1), along with-N-benzoylphenylalanine--2-benzamido-3-phenyl- propyl ester (2), indole-3-carboxylic acid (3), apigenin (4), diosmetin (5) and luteolin (6) was isolated. CONCLUSION: Compounds 1-5 were isolated for the first time from Vernonia patula.

结果：分离到1个牻牛烷型倍半萜内酯， 1个苯丙氨酸衍生物、1个生物碱和3个黄酮化合物，根据波谱数据和X-衍射分析分别鉴定为：incaspitolide D (1)，-N-苯甲酰基苯基丙氨酸–-2-苯甲酰氨基–3-苯基丙酯(S-N-benzoylalanine-–2-benzamido-3-phenylpropyl ester， 2；吲哚-3-羧酸(indole-3-carboxylic acid， 3)；香叶木素(diosmetin， 4)，芹菜素(apigenin， 5)和木犀草素(luteolin， 6)。

Cytokines found to have up-regulated in cells were measured in serum and lung tissue of rats exposed to large tidal volume ventilation. For investigating the intracellular pathway of cytokine up-regulation in VILI, exogenous TNF-α or H2O2 was added to culture media of alveolar epithelial cells. Cytokines were then measured. To explore the role of oxidant stress in VILI, N-acetylcysteine, as an anti-oxidant, was used in vitro and in vivo.

我们在体外培养的肺上皮细胞中加入外源性肿瘤坏死因子、过氧化氢或N-乙酰半胱氨酸，观察细胞因子的变化。N-乙酰半胱氨酸亦被投入大鼠呼吸机诱导肺损伤模型，观察用药后细胞因子的变化。

Histological observation showed that degeneration,necrosis of liver cells,edema,extravasated blood,erosion and degranulation of the mast cells in intestine mucosa.

肠谷氨酰胺酶活性降低，肠粘膜对谷氨酰胺利用减少，肠粘膜通透性增加。组织细胞学显示，TAA组动物肝细胞发生严重变性坏死；肠粘膜淤血、水肿、糜烂，肠肥大细胞脱颗粒。

It is abound of GLN, which is an important energy source for the growth of enteric flosses, keeping their activity, increasing the absorbment of nutrition and resisting diarrhea.

富含谷氨酰胺二肽，科学研究表明：谷氨酰胺是肠道绒毛生长的主要能量来源，保持绒毛的活性，增加营养物质的吸收和抗拉稀。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。