酰氨

Escherichia coli leucyl-tRNA synthetase was overproduced and purified from an overexpression strain.

采用含pTrc-99B/leuS2转化子的高表达菌株，简便快速地提取、纯化大肠杆菌亮氨酰-tRNA合成酶。

Theanine, the ethyl amide of glutamic acid, is the main amino acid in the fresh leaf and contributes 1 to 2 percent of the dried weight, but other amino acids arise during the early part of the processing.

茶氨酸是谷氨酸的乙酰胺，它是鲜茶叶中的主要氨基酸，占干茶叶重量的1~2%，其它氨基酸则是在加工早期出现的。

The fluorescent emission mechanism, and the some factors that affect the energy and the intensities of the fluorescent peaks were studied. The strong powder second harmonic generation efficiencies for complexes (1),(2),(3) and (4) were found. The determination of variable-temperature magnetic susceptibility for complex (3) and complex (5) was performed. The magnetic data were analyzed with their structure properties, and the further information about the magnetic coupling capacity of Carbamyldicyanomethanide as bridging ligand was obtained.

测试了配合物(1)，(2)，(3)，(4)的荧光光谱，从配体的结构特点等方面研究了该荧光光谱的产生机理，研究了影响荧光谱峰能量及强度的因素；发现了配合物(1)，(2)，(3)，(4)显示有强的二阶非线性倍频效应；测试了配合物(3)和(5)的变温磁化率，对所得磁数据进行了结构性质分析，进一步获得了甲氨酰二氰基甲基阴离子作为桥联配体的磁耦合信息。

OBJECTIVE: To review the recent advances in research of peptidyl-prolyl cis-trans isomerase Pin1 in tumor.

目的：总结肿瘤中肽基脯氨酰顺反异构酶Pin1的研究进展。

The chaperone activity of porcine kidney peptidyl-prolyl cis-trans isomerase was studied. The results showed that inactive or active PPIase was able to improve the refolding yields of creatine kinase , and inhibited aggregation. However, alkylated PPIase was not able to prevent CK aggregation.

研究猪肾脯氨酰顺反异构酶的伴侣功能时发现有活性和无活性PPIase能够提高肌酸激酶再折叠产量，抑制肌酸激酶聚沉，但是烷基化PPIase丧失防止肌酸激酶聚沉的能力。

The chaperone activity of porcine kidney peptidyl-prolyl cis-trans isomerase was studied.

研究猪肾脯氨酰顺反异构酶的伴侣功能时发现有活性和无活性PPIase能够提高肌酸激酶再折叠产量，抑制肌酸激酶聚沉，但是烷基。。。

MS detection showed the six protein spots were inorganic pyrophosphatase, peptidyl-prolyl cis-trans isomerase A, elongation factor Tu, unnamed protein, dioxygenase and moxR protein. Conclusion Protein expression differences were discovered between multi-drug-resistant Mycobacterium tuberculosis isolate and drug-sensitive isolate.

质谱分析发现，6个蛋白质点分别代表的蛋白质为无机焦磷酸酶、多肽脯氨酰基顺反同分异构酶A、延伸因子Tu、420个氨基酸的未知功能蛋白质、双加氧酶和转录调节蛋白。

The effect of hydrochloric acid hydrolysis of soybean cake powder and cottonseed powder under different pressure were investigated on MTGase production by Streptomyces.

研究了常压及高压条件下以豆饼粉、棉籽粉酸水解液作氮源，对链霉菌 HS-1产转谷氨酰胺酶的影响。

METHODS: Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells. DMEM medium containing fetal bovine serum, penicillin, streptomycin and L-glutamine was added. Following several adherences and purification, the floating cells were discarded. Thus, many adherent cells with a confluence were collected. When cells were 60%-80% confluent, cells were digested by trypsin for subculture.

无菌条件下，应用Percoll密度梯度离心法分离脐血标本，收获中间层细胞，加入含胎牛血清、青霉素和链霉素、L-谷氨酰胺的DMEM基础培养液，再经反复贴壁纯化，除去悬浮生长细胞，得到较多呈融合状态的贴壁细胞，待细胞达60%~80%融合时胰酶消化传代。

Can with water, alcohol and ammonia reaction, difference generate acids, esters and amides, the reaction rate than the corresponding ester and anhydride fast.

可与水，醇和氨反应，分别生成酸、酯和酰胺，反应速度比相应的酯和酸酐快。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。