酰氨

SSCP was detected in exon 6 and exon9p of the gene, but not in the other exons.Results In polyacrylamide gel electrophoresis, We have not found abnormal conformer in both affected individuals and control cases. There were no differences in the DNA sequence between these two groups.

结果 在非变性聚丙烯酰氨凝胶电泳中，未发现DCM组和对照组该基因的外显子6和外显子9p出现异常构象，通过DNA测序证实两组的核苷酸序列没有差异。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

And it has also been demonstrated that acid sphingomyelinase, an enzyme responsible for hydrolyzing sphingomyelin to generate ceramide, could be activated at the same time.

同时，一种可以水解鞘脂类物质产生神经酰氨的酸性鞘磷脂酶往往在此过程被激活，这些过程对于胞内信号转导具有重要的作用。

Lactic dehydrogenase isoenzyme of deferent tissue and organ of china wood frog in changbai moumtain were studied by PGE,the result show that the most of enzyme bands are twelve,the less are seven.

用聚丙烯酰氨凝胶电泳对长白山中国林蛙的不同组织器官的乳酸脱氢酶的同工酶进行了分析，结果显示出最少为 7条酶带，最多为 12条酶带。

In contrast, the levels of glutamate and alanine decreased, the level of ghitamine increased in the ipsi-lesional MVN, and the level of glutamate decreased in the contra-lesional MVN of rats 2 weeks after infusing 10 mg arsanilic acid into the tympanic cavity to obstruct m-periphery vestibular organ.

结果提示，单侧迷路破坏后双侧MVN区氨基酸含量立即失去平衡，随着前庭代偿的进展，此差异减少，但是在前庭代偿后却出现双侧前庭核区谷氨酰氨的含量失衡，说明在前庭代偿过程中氨基酸含量变化起着重要作用。

Binding affinities of a series of benzamide inhibitors for trypsin were evaluated by molecular dynamics simulations using a linear response approach.

用基于线性响应近似的自由能预测方法计算胰蛋白酶和苯酰氨类抑制剂的结合自由能。

The Cry1Ac5 domainⅢhas a distant relationship with other Cry1 proteins and its longβ18-β19 loop possesses a unique tryptophanat residue 544 and a unique asparagineat the loop apex(residue 546),as well as two consecutive serinesfrom residue 547 to 548.Alanine scanning mutations within the loop were initially generated and all mutants could produce bipyramidal crystals and express 130 kDa protoxins.

利用Swiss-Model服务器和Swiss-pdb Viewer软件建立了Cry1Ac5蛋白的三维结构模型，并利用Clustal W和DS ViewerPro软件对比分析了其与其它Cry蛋白之间的差异，发现Cry1Ac5的结构域Ⅲ与其它Cry1蛋白的亲缘关系较远，其结构域Ⅲ上的β18-β19 loop比Cry1Aa、Cry2Aa、Cry3Aa和Cry4Aa蛋白对应的loop长，其组成的残基中色氨酸(W544)、连续的丝氨酸(S547、S548)和loop顶点的天冬酰氨(N546)在Cry蛋白中都是独特的残基分子成分。

Objective To explore the effect of NMDA receptor antagonist glutamyl amino-methyl phosphonic acid on the bilirubin neurotoxicity.

目的 探讨兴奋性氨基酸受体拮抗剂磷酸甲基谷氨酰氨酸对胆红素神经毒性的影响。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导，建立了MG63/R，其对顺铂的耐药指数为83.557±4.841，对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药；光镜观察可见MG63/R细胞排列不规则，形态呈三角形、多角形及多核现象；透射电镜显示MG63/R细胞表面突起增加，粗面内质网丰富；细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低；MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加，P53表达则明显降低。

Effects of microbial transglutaminase on the tensile properties of plastic made from soy protein isolates are studied. The results show that the tensile strength and Young modulus of the protein plastic are increased from 9.2MPa and 265.4MPa of the control, which is not treated with TG, to 14.7MPa and 390.1Mpa respective, which is treated with TG at 50℃ for 60min. High molecular weight protein conjugates are formed with TG treatment, and they are responsible for the enhanced tensile properties, denaturalizing temperature and enthalpy values.

研究了微生物谷氨酰氨转胺酶对大豆分离蛋白塑料拉伸性能的影响，发现在50℃时转氨酶对大豆分离蛋白催化聚合反应60 min，形成的高分子聚合物使大豆分离蛋白的抗拉强度和杨氏模量分别由未经处理时的9.2 MPa和265.4 MPa增加到14.7 MPa和390.1 MPa，同时增加了蛋白质的变性温度和热焓值。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。