酯酶

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

Four isozymes: esterase, peroxidase,α-amylase and superoxide dismutase extracted from buds of 16 radish (Raphanus sativus L.) accessions were studied with polyacrylamid gel electrophoresis.

采用聚丙烯酰胺凝胶电泳技术对16份萝卜种质花蕾的酯酶、过氧化物酶、α-淀粉酶和超氧化物歧化酶同工酶进行系统分析。

C Relationship among subfamilies based on EST characters is somewhat compatible to intrafamily phylogeny proposed by Schuh (1976), but it is clear that Orthotylinae and Phylinae are not directly sister grouped. The relationship should be corrected into ( Phylinae Orthotylinae .

3酯酶同工酶酶谱特征所反映出的盲蝽科4个亚科间的相互关系与Schuh 1974年所做的盲蝽科内系统发育部分一致，但合垫盲蝽亚科与叶盲蝽亚科之间不是直接的姐妹群关系。4个亚科的关系为（（（盲蝽亚科Mirinae，齿爪盲蝽亚科Deraeocorinae）叶盲蝽亚科Phylinae）合垫盲蝽亚科Orthotylinae）。

Biotype identification of the NJ strain of whitefly, Bemisia tabaci The biotype of the NJ strain of whitefly collected in Nanjing, China, was identified with biological, biochemical and molecular approaches. The NJ strain can induce squash silverleaf symptom by infecting.

一、烟粉虱生物型鉴定利用西葫芦接虫法、酯酶同功酶电泳和线粒体细胞色素氧化酶Ⅰ基因序列比较等方法对南京品系烟粉虱生物型进行了鉴定。

After gestational periods the activities of SOD,MDA,GSH-Px,TChE and CAT in brain were analyzed respectively.

采用饮水染毒，染毒时间为21d，分娩后每窝随机选取6只乳鼠测定脑匀浆超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、乙酰胆碱酯酶活性和丙二醛的含量。

However, fully consideration of special structure and physiological function of CRP is critical to investigate whether CRP provide protect effect or pathogenic effect in the progression of atherosclerosis. CRP is a member of pentrxins family, which consists of five identical, noncovalently associated 23-kDa protomers arranged symmetrically around a central pore with a 102 A external diameter. Each protomer has a recognition face with a phosphocholine binding site consisting of two coordinated calcium ions adjacent to a hydrophobic pocket. The opposite face of the pentamer is the effector face, where complement Clq binds and Fc receptors are presumed to bind. A cleft extends from the center of the protomer to the central pore of the pentamer. Both faces ofpentamer form a special bi-phase structure: one face is the ligand recognition phase, which can recognize apoptosis cells and enzyme modified low density lipoprotein in which PCh is exposed.

CRP是一种五聚体蛋白，外径为102 A，由5个相同的单体以非共价键结合，形成双面的环形结构，每个单体的配体识别位点或受体结合位点分别位于五聚体两个平面上，分别组成配体识别相和效应器相，从而构成特殊的二相性结构：一面为配体识别相，含有磷酸胆碱(phosphorycholine，PCh)结合位点，能够使CRP识别在病理条件下暴露出PCh的凋亡细胞、酶修饰低密度脂蛋白(enzyme modified low density lipoprotein，E-LDL)等。E-LDL是天然低密度脂蛋白(native Low density lipoprotein，N-LDL)在多种蛋白酶，如胰蛋白酶、神经氨酸酶、胆固醇酯酶等作用下的代谢产物，其主要特点是结构发生改变(N-LDL大小均匀，平均直径250±30 A，E-LDL大小差异极大，直径为100-2000 A)，暴露磷酸胆碱位点，能够被CRP识别；另一面为效应器相，能够与巨噬细胞、单核细胞表面受体FcγRⅡa结合，介导巨噬细胞、单核细胞识别及吞噬凋亡、坏死细胞作用。

By now, there is seldom report on the Heilongjiangwood frog which hibernates at low temperature condition. Our study mainly analyze theadaptation changes of liver proteins, SOD, LDH, G6PDH and EST activities of Heilongjiangwood frog under low temperature.

本文旨在研究低温条件下黑龙江林蛙肝脏蛋白质、超氧化物歧化酶、乳酸脱氢酶、葡萄糖6-磷酸脱氢酶(G6PDH)及酯酶的适应性变化，以探讨黑龙江林蛙冬眠期低温耐受的机制，为两栖类低温生物学和比较生理学的深入研究提供理论参考和实验依据。

Moreover, the isozyme of larvaes in different instars and adults with different sex were also different.

结论］不同发育时期黑色型黄粉虫的酯酶和过氧化物酶同工酶存在差异，表现出不同的生理特征。

The research aimed to study esterase isozyme and peroxidase isozyme of black worm Tenebrio molitor Linnaeus in different developmental stages or instars, and thus provide a theoretical basis for species identification and large-scale farming.

［目的］对黑色型黄粉虫各虫态及龄期的酯酶和过氧化物酶同工酶进行研究，为其物种鉴定和大规模饲养提供理论依据。

Activities and isozynies of POD and EST were analyzed with materials from different developmental stages of wheat original strains and their radiation mutants, and the relations betweens biological characters and isozymes were studied.

采用不同发育时期的小麦辐射突变体及原始品种材料对其酯酶、过氧化物酶酶谱进行了比较研究，并对一些农艺性状之间的相关性进行了测定。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。