酯酶

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组，采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能，检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变，检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片，检测NOS、CHE光密度值；流式细胞技术：检测细胞凋亡率，及促细胞凋亡基因、P_(53)蛋白表达；病理切片采用HE染色、PAS染色及免疫组化法检查，检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

PAGE was used to analyze and compare the variation of composition and content of four isoenzymes , esterase, acid phosphatase, malate dehydrogenase and lactate dehydrogenase, of Paramecium bursaria which cultured in light and dark .

作者应用聚丙烯酰胺凝胶电泳技术分析比较了光培养绿草履虫和暗培养绿草履虫中酯酶、酸性磷酸酶、苹果酸脱氢酶和乳酸脱氢酶四种同工酶的组分及含量的变化。

Assulta and the induction of GST activity by nicotine and capsicine in H. assulta provided partial supports for the hypothesis that diet breadth was governed by detoxification abilities. The results also indicated that two sibling species had different strategies of adaptation to toxic substances: H. assulta saves expenditure of nutrients and energy by restricting their countermeasures to a few potentially toxic compounds. H. armigera, by contrast, must be prepared to counter a wider range of potential toxins. 5. Compared with parental species, hybrids of reciprocal hybridization had significant difference in feeding preference for hot pepper.

结果显示，广食性的棉铃虫在中肠羧酸酯酶和谷胱甘肽-S-转移酶活性方面高于寡食性的烟青虫，但烟青虫谷胱甘肽-S-转移酶活性可以被寄主植物内的烟碱和辣椒素所诱导，表明解毒酶活性在一定程度上影响着植食性昆虫的食性广度；同时也暗示着二近缘种对有毒的次生物质采取不同的适应策略：烟青虫有选择地对付种类较少的植物次生物质以减轻营养和能量的消耗；棉铃虫则必须对付存在于其寄主植物内的种类繁多的有毒次生物质。

The purpose of this study is to reveal the change characteristics of biological properties of sandy soil and its development trend under the establishment of C. microphylla plantations in flowing sand dune. With semi-moving sand dune and natural community as controls, the activities of phosphomonoesterase, saccharase, protease, urease, dehydrogenase and the Contents of microbial biomass C, N, P of the soil in an age sequence (5-, 10-, 22-year) of C.

为探讨采用小叶锦鸡儿固定流沙后，土壤生物活性的变化特点和发展趋势，选取5、10、22年生小叶锦鸡儿人工固沙群落为对象，以半流动沙丘和天然群落为对照，研究了人工固沙群落发育过程中土壤磷酸单酯酶、蔗糖酶、蛋白酶、脲酶、脱氢酶的活性和微生物生物量C、N、P质量分数的变化特征。

The zymogramatic variations of esterase isoenzyme and superoxide dismutase isoenzyme of 20 sample materials, including 5 species, 2 varieties, 1 multiploid of Isatis L. and 1 outgroup species, Coronopus didymus J. E. Smith, were analyzed with technique of polyacrylamide gel electrophoresis.

采用聚丙烯酰胺凝胶电泳，比较了菘蓝属5种2变种1多倍体品种及1外类群种共计20个样本的酯酶同工酶和超氧化物歧化酶同工酶的酶谱差异，并运用数量分类学的原理和方法对酶谱数据进行了聚类分析。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71％and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54％and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52％,and the activity of penicillin G acylasewas in creased by about 76％;about 90％of Hexameric Calcitonin(Cal6)inclusion body and l5％of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中，周质空间分泌蛋白总量较对照组提高了约71％，GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍，碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54％；在过量表达GroEL的宿主菌中，周质分泌蛋白总量较对照组提高了约52％，青霉素G酰化在周质空间酶的活力较对照组提高了约76％，鲑鱼降钙素六聚体的可落性组分的比例由原来的45％增加到约90％，而MS2-人白介素-3融合蛋白的包涵体有约15％转变为可溶性组份。

Studied the stachydrine dealing with the Oncomelania hupensis's esterase、the glycogen and the total protein. The result showed when dealt with the Oncomelania hupensis for 1~2 days, the figures of enzyme are higher than the compared group; when dealt for 3~4 days, the figures of enzyme reached the highest level; when dealt for 5 days, the figures of enzyme remarkably reduced.

用益母草中水苏碱处理钉螺导致其酯酶同工酶、糖原及总蛋白变化的实验结果表明：处理1~2d后钉螺的酶活高于清水对照组，处理3~4d后钉螺的酶活最强，而处理5d后钉螺样品的酶活则大大减弱。

Activities of polyphenoloxidase, true choline esterase, glutathione S transferase, superoxide dismutase and catalase of the beet armyworm, Spodoptera exigua, were tested in vivo in a relatively susceptible population kept in the laboratory and a population collected from vegetable field in Wuhan.

用一个采自武汉地区的甜菜夜蛾田间种群和一个相对敏感种群，比较了甜菜夜蛾的表皮酚氧化酶、乙酰胆碱酯酶、谷胱甘肽S转移酶、超氧化物歧化酶和过氧化氢酶酶活力差异。

(Times New Roman小四号,加粗,顶格)Using the vertical polyacrylamide gel electophoresis, the differentiation of peroxide, catalase and esterase isoenzyme from roots, caudexes and leaves of three wild buckwheat were analyzed.

(宋体小四号，加粗，顶格)采用垂直板聚丙烯酰胺凝胶电泳对三份野生荞麦根，茎和叶的过氧化物酶，过氧化氢酶和酯酶的同工酶进行了酶谱分析。

The electrophoretograms of isozymes from five different tissues （foot, mantle, adductor muscle, gill, liver） of Mercenaria mercenaria Linnaeus were analyzed, by vertical polyactylamide gel electrophoresis.

采用垂直板聚丙烯酰胺凝胶电泳技术，对硬壳蛤5种组织（足、外套膜、闭壳肌、鳃和肝）的酯酶、苹果酸脱氢酶和乳酸脱氢酶同工酶进行了比较研究，并对酶谱表型及位点表达进行了分析。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。