酯酶

The asymmetric esterification of glycidol catalyzed by lipase was carried out in a nonaqueous medium. The effect of the replacement of water necessary for the function of lipase with water-mimicking solvent on the resolution was investigated.

利用脂肪酶在非水介质中对外消旋环氧丙醇进行不对称酯合成反应，重点研究了强极性有机溶剂作为仿水溶剂完全替代反应体系中的微量&必须&水对酶促拆分反应的影响。

Eight lipase-producing bacterium strains were screened from 40 cold-adapted strains, these strains isolated from the mud of Parece Vela sea basin in the Pacific Ocean of 5010m deep, of which two similar strains have the highest lipolytic activity and are positive to olive oil.

通过三丁酸甘油酯平板法从太平洋帕里西维拉海盆5010m的底泥中共筛出八株可产脂肪酶的菌株，其中的两株生理生化特征极其相近的菌脂肪酶活性最高，并且对橄榄油平板产生荧光。

Pharmacodynamics experiments indicated that the drug obviously degraded the content of Cholesterol,Triglycerides,glutamic pyruvic transaminase and Maleic Dialdehyde in the Alcoholic fatty liver of rats and mice treated with by oral administration, it also degraded the content of glutamic oxalacetic transaminase, r-GLU,alkaline phosphatase and increased the content of Albumin and Superoxide Dismutase in the Alcoholic fatty liver of rats, and remarkably reduced depressant effect of the mice'centra nerve.

药效学实验表明，经预防灌胃给药，可以显著降低酒精性脂肪肝大鼠和小鼠，总胆固醇、甘油三酯、谷丙转氨酶、丙二醛含量，降低酒精性脂肪肝大鼠谷草转氨酶、γ-谷氨酰基转移酶、碱性磷酸酶含量，增加白蛋白和超氧化物歧化酶含量。

Its optimum pH and temperature are 5 and 40℃ respectively. 2 Solubility and Kinetics Characteristics of Tannase in RM System 2. 1 A method of HPLC was set up to assay the content of gallic acid and alkyl gallate at the same time.

反胶束单宁酶的动力学性质研究 2.1 建立了HPLC同时测定反胶束酶催化体系中底物没食子酸和产物没食子酸烷基酯含量的方法。

However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

而传统的三丁酸甘油酯平板缺乏有效营养成分，重组酵母无法生长；橄榄油平板灵敏度较低，低脂肪酶活力的重组酵母不能产生变色圈，这给产脂肪酶基因工程菌的鉴定带来了困难。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin，TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Westernblot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin， TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中，重组酵母S78-pVT102U／α-lip摇瓶发酵72h，酶活可达20U／ml；S78-pVT102U／α-L-lip07表达的脂肪酶很少，表达产物仅在三丁酸甘油酯平板上形成透明圈。

One integrative transformant was obtained through PCR and phenotype identification, designated as A.oryzae ONL1. The 7-day fermentation broth of A.oryzae ONL1 could hydrolyze tributyrin verified by forming transparent cleared zone on tributyrin plate, and the enzyme activity reached 2.5 U/mL. The SDS-PAGE electrophoretic map of the fermentation broth demonstrated that there was a typical RML band at 32.5 kDa.

其培养7天的培养液上清在以三丁酸甘油酯为底物的平板上形成清晰的水解透明圈，碱滴定法酶活测定表明培养液酶活可达2.5 U/mL，培养液上清的SDS-PAGE图谱在32.5 kDa处有RML的特征条带。

In this paper, the techniques to improve the quality and the application of monascus esterifying enzyme were illustrated.

从酶学特性出发对提高酯化酶产品生产质量和应用技术进行了论述。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。