酯酶

A hard butter rich in 1,3—distearyl-2-lonoleyl-glycerolhas been prepared by lipase-catalysed transesterification of safflower oil with stearic acid.

用高亚油酸型的红花籽油和硬脂酸，经1，3位专一性固定化脂肪酶Lipozyme的催化，通过有方向性的酯交换，制备出了以1，3——二硬脂酰—2—亚油酰甘油酯为主要组分的硬脂。

During these years (1997-2000) in the research area of annonaceous acetogenins 5 natural products and a number of chiral-pure analogs of annonaceous acetogenins have been synthesized using sugars as the starting materials. Their high activity and selectivity against cell line of colon tumor and a relationship between activity and configuration have been found too. In the respect of inhibitor of sialidase two new methodologies for the synthesis of chiral-pure 3-deoxy-ulosonic acid using hetero-Diels-Alder reaction or asymmetric propargylation as the key reaction were developed. On the topic of anti-leukemia marine natural product clavulactone a synthetic method based on the free radical cyclization and starting from sugar has been developed. In other title of PKC inhibited agent sphingosine a new synthetic route with SN2' as the key reaction has also been disclosed.

项目期间（1997-2000）在番荔枝内酯方面从糖出发合成了五个天然产物和一批手性纯的类似物，它们对肠癌细胞有很高的活性和选择性，并与构型有关；在唾液酸酶抑制剂方面发展了二类全新手性纯高碳糖的合成方法－杂原子D－A反应或丙炔基不对称加成为关键的反应；抗白血病海洋天然产物群柱虫内酯方面研究了由糖出发和自由基环化反应的合成方法；对PKC 起抑制作用的鞘氨醇方面也开创了新的合成途径－由糖出发并以SN2'反应为关键反应。

Several high-temperature monascus strains screened from high-temperature Daqu (used for Maotai-flavor liquor production in Langjiu Group were used to study the esterification conditions of their raw zymin.

以从郎酒厂酱酒生产用高温大曲中分离筛选出几株产酯能力相对较强的高温型红曲霉为菌种，对其粗酶制剂酯化条件进行初步研究。

The enzymatic digestibility of native and acetated tapioca starches with different degree of substitution was investigated in in-vitro digest model stimulated the human intestine tract and the resistant starch content was determined using Megazyme total starch assay kit.

采用生物体外消化模型模拟人体消化道环境，对不同取代度的醋酸酯化木薯淀粉的消化速率进行了研究；用微生物酶对醋酸酯化木薯淀粉进行生物降解并测定各个样品的抗消化淀粉含量。

The by-product including yellow water and ending water in liquor production was used to produce esterifying liquid through the addtion of ending liquor,Daqu,quality aged pit mud,fermented grains,quality edible alochol,caproic acid and esterifying enzyme and yeast for liquor use only etc.

以白酒生产的副产物黄水和尾水，添加酒尾、大曲、优质老窖泥、酒醅、优质食用酒精、己酸、酯化酶和酒用酵母等生产酯化液。

Daphne diterpene esters are principal activitive components of Daphne genkwa, which is an endemic traditional Chinese medicine. Yuanhuacine has potent inhibition against several cancer cell lines. Inhibition against DNA topoisomerase I is probably its anticancer mechanism.

芫花为中国特有的瑞香科植物，主要有效成分为植物中罕见的瑞香烷型二萜原酸酯类化合物，其中芫花酯甲对多种癌细胞系有较强的抑制活性，对DNA拓扑异构酶I的抑制作用是其抗癌的可能机理。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

When lipase N435 was 2.5gL^(-1), RS-ethyl mandelate was 0.25gL^(-1), water to RS-ethyl mandelate was 5:1, the reaction temperature was 40℃, the speed of agitation was 200rmin^(-1), the R-ethyl mandelate conversion could reach 41.6%, and enantiomeric excess e.

确定了最适的反应条件：脂肪酶N435浓度为2.5gL^(-1)，RS-扁桃酸乙酯浓度为0.25molL^(-1)，水：RS-扁桃酸乙酯的摩尔比为5：1，反应温度为40℃，摇床转速为200rmin^(-1)，反应时间为24h。

The enzyme tannase, which is used in the present invention, is known to hydrolyse the galloyl ester linkages of polyphenolic substrate s such as tannic acid, tea polyphenol gallates, and the like.

我们知道，这种在本发明中所使用的丹宁酶，可水解多酚酸类物质的棓酸酯键，如丹宁酸，茶叶的棓酸多酚酯及其类似物。

Lipase production on agar medium was detected by the tributyrin diffusion plate assay.

单甘酯合成试验表明菌株D2所产的脂肪酶具备合成单甘酯的能力。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。