酯酶

The esterase isozyme in leaf at acicula forming stage and the peroxidase isozyme in peanut leaf at pod producing stage was related to the formation of coarse protein and coarse fattiness of seed.

下针期花生叶片的酯酶同工酶与结荚期叶片过氧化物酶同工酶与其果仁粗脂肪、粗蛋白的形成有关；饱果期花生叶片和果仁酯酶同工酶与花生的单株果数和果重相关，并且对花生果重、仁重有一定的影响。

The activities of carboxylesterase、glutathion-S-transferase and acetylcholinesterase per insect increase greatly and the properities of esterase and acetylcholinesterase change also throughout larval development from 1-day-old to 4th instar. The affinity for Asch and the sensitivity to insecticide of acetylcholinesterase in 3rd instar larvae are significantly lower than that in 4-day-old larvae.

幼虫从1日龄生长到4龄时，单头幼虫的羧酸酯酶、谷胱甘肽转移酶、乙酰胆碱酯酶活力急剧增长，4龄幼虫相对于1日龄幼虫，HJ-R、HD-S上述3种酶系的活力分别增长了148、172.6、65.6倍和203、60.9、77.4倍；生长过程中，全酯酶、乙酰胆碱酯酶的性质也发生变异，特别是从4日龄生长到3龄时，乙酰胆碱酯酶的米氏常数、双分子速率常数均发生明显的改变，3龄幼虫乙酰胆碱酯酶对底物的亲和力以及对杀虫剂的敏感性显著低于4日龄幼虫。

Erysimi CarE activity both of resistant populations and of susceptive population were trend to normal school, and the resistant L. erysimi populations have significant higher CarE activity than susceptive L. erysimi population. Thereinto, the activity level of CartE in Hongtang L. erysimi population is the highest among the four field populations, which is tally to the result of drug-fast test on each population.

对各地菜缢管蚜种群个体羧酸酯酶研究结果表明，无论是抗性种群还是敏感种群，其个体羧酸酯酶活性分布频率均趋于正态分布，即两头低，中间高；各抗性种群的羧酸酯酶的活性水平明显高于敏感种群，其中以洪塘菜缢管蚜种群的羧酸酯酶活性水平最高，这跟各种群的抗药性监测结果亦相吻合。

The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P＜0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P＞0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6％final gel concentration and there were 15～19 bands identified on this condition.

同功酶电泳图谱显示，1℃组与室温组黑龙江林蛙肝脏LDH酶活性差异不显著，均以LDH-5为主，但1℃组LDH-1显著高于室温组(P＜0.05)，推测黑龙江林蛙为适应低温低氧环境，增强LDH-1酶活性以较好的利用乳酸，避免因大量乳酸形成造成肝脏疲劳；而1℃组肝脏G6PDH酶活性无显著变化，进一步说明低温条件下葡萄糖戊糖途径和糖酵解作用的互相协调，维持机体代谢的稳定；1℃组和室温组EST总酶比活力无显著变化，但1℃组中R_f=0.17和R_f=0.72的EST同工酶显著升高(P＜0.05)，推测在低温条件下黑龙江林蛙可能通过选择性增强部分酯酶同功酶活性，增加肝脏酯类物质代谢，为机体提供维持生命必需的最低能量。

In the field of the agriculture, the organic phosphor and the amido formylesters were the insecticides which were broadly used for a long time.

根据胆碱酯酶对乙酰胆碱和丁酰胆碱的选择性水解作用可分为乙酰胆碱酯酶和丁酰胆碱酯酶。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

The researchs on isozymes indicated that some varieties of isozyme loci present during the embryo development. There are 7 esterase loci and 5 acid phosphatase loci during the periods between nucellar filament and early globular embryo; both of Est and Acp appeared the most diversity in the stage of 8-nucleate embryo sac.

用聚丙烯酰胺凝胶电泳的方法研究鹤顶兰从指状突起出现到早期球形胚形成的全过程中酯酶、酸性磷酸酶的变化发现：这一过程中酯酶表达有7个同工酶位点，酸性磷酸酶有5个同工酶位点，两者均在八核胚囊时期变化最显著。

The synergism ratios of piperonyl butoxide to alpha-cypermethrin in these three strains were similar(about 20-fold), which suggested metabolic detoxification probably confers about 20-fold inherent resistance in the B-type whitefly, as PBO is a mixed function oxygenase and esterase inhibitor and glutathione S-transferase is not affinity to resistance in B. tabaci. The synergism ratio of triphenyl phosphate to alpha-cypermethrin in the NJ strain was 12-fold, which implied the synergism of PBO in the B-type whitefly is mainly due to inhibiting esterase activity.

增效试验表明PBO对这三个品系的增效作用均为20倍左右，因为在烟粉虱中PBO即是多功能氧化酶抑制剂，也是酯酶抑制剂，而谷胱甘肽S-转移酶与抗性无关，因此推断代谢酶在B型烟粉虱对高效氯氰菊酯的抗性发展中起到的相对作用可能为20倍左右，而且这种抗性是B型烟粉虱天生的；TPP对NJ品系的增效作用为12倍，说明PBO增效作用主要是由于抑制酯酶而引起的，即代谢抗性大部分由酯酶所贡献。

This paper discusses research progress in acetylcholinesterase molecular shape and three-dimensional structure, gene structure, and the relationship between allosteric acetylcholinesterase and pesticide resistance, as well as utilization of the molecular domain of acetylcholinesterase.

文章介绍了乙酰胆碱酯酶的分子形式、三维结构、基因结构，变构乙酰胆碱酯酶与抗药性的关系以及乙酰胆碱酯酶分子领域应用等方面的研究进展，并对乙酰胆碱酯酶的进一步研究提出了展望。

The leaf esterase,endosperm esterase, root esterase and coleoptile esterase zymogram phenotypes in Set II alien addition Lines of wheat-wheatgrass, octoploids of wheat-wheatgrass, common wheat and wheatgrass were analyzed.

本文分析了小冰麦异附加系列II、八倍体小冰麦、普通小麦和天兰冰草的叶片酯酶、胚乳酯酶、根酯酶和胚芽鞘酯酶和酶谱表型。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。