酪蛋白酸

The diets of test group 1, 2 and control group included in 0.5% enzymatic casein (small peptides above 80% and average length of peptides is 2.98), 0.5% acidify hydrolyzed casein(free amino acids above 80%) and 0.5% casein.

试验期56天。结果表明：添加O.5％酶解酪蛋白组草鱼其相对生长率、净蛋白沉积率、饲料系数和血浆中镁含量与小肽总量均显著高于添加0.5％酪蛋白组和0.5％酸解酪蛋白组(p＜0.05)。

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3- and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the P-selectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3-and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the Pselectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

The amino acid sequence similarity of C3d between human, bovine, goat, boar, golden hamste,rmouse,brown rat,Macropus, hare,Gallus is 80.4%,94.7%,96.9%,82.8%,81.1%,80.6%,80.2%,71.0%,75.2% and60.3% respectively..

通过生物学软件分析发现绵羊C3d蛋白有2个蛋白激酶C磷酸化位点，1个酪蛋白激酶Ⅱ磷酸化位点，1个酪氨酸激酶磷酸化位点，1个十四酰化位点。

These changes were prevented by genistein (a protein tyrosine kinase inhibitor) and antioxidant N-acetyl-L-cysteine, but promoted by sodium orthovanadate (a protein phosphatase inhibitor), which were administered to the SD rats 20 min before ischemia.

进一步的研究表明，缺血前20 min腹腔注射给药，然后缺血30 min，发现蛋白酪氨酸激酶抑制剂染料木黄酮和抗氧化剂N-乙酰半胱氨酸能显著地抑制核内STAT3的磷酸化水平及DNA结合活性的增加(磷酸化水平从2.3和2.5倍分别降为1.2和1.4倍， DNA结合活性则从2.8和3.7倍分别降为1.1和1.5倍)，而蛋白酪氨酸磷酸酶抑制剂矾酸钠则能明显地促进他们的增高(磷酸化水平从2.0倍增到3.4倍， DNA结合活性从3.1倍增为5.1倍)。

Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中，发现rottlerin抑制鸟胺酸去羧化活性最具成效，之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性，因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后，细胞进行计划性死亡，并可侦测到DNA片段、细胞核浓缩及sub-G1提增；而细胞处理药物十小时后与控制组细胞比较，观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上；除此以外，测定鸟胺酸去羧化去磷酸化的情况，发现於酥胺酸位置有显著差异，而丝胺酸和酪胺酸位置并无差异。

The effect factors of solid content, alkali, reaction temperature, reaction time, cross-linking agent, starch, polyvinyl alcohol, termination reaction point, resistivity against water, alkali washfastness on the performance of the adhesive for labeling beer bottle were investigated.

采用乾酪素、聚乙烯醇、酸变性淀粉为主要原料，制备改性酪蛋白啤酒瓶标签胶，讨论了固含量、碱、反应温度、反应时间、交联剂、酸变性淀粉、聚乙烯醇、终点判断、耐水性、碱洗净性等因素对酪蛋白商标胶的制备与性能的影响。

Acceptable resolutions were achieved when it was applied for the separation of dipeptides including Leu-Tyr and Val-Tyr by using 1-butanol-acetic acid-water (4:1:5, V/V/V) solvent system. The proteins including cytochrome C and myoglobin, lysozyme and myoglobin, and fresh chicken egg-white proteins were well separated by 12.5% PEG1000-12.5% K2HPO4-75% water (pH 9.0) and 16% PEG 1000-12.5% K2HPO4-71.5% water (pH 8.0) system.

应用研究表明，采用正丁醇-醋酸-水(4：1：5， V/V/V)和PEG1000-磷酸钾盐-水(12.5：12.5：75， W/W/W)(pH 9.0)体系可以在较高的流动相流速和较高的固定相保留下分别实现对亮氨酸-酪氨酸和缬氨酸-酪氨酸二肽混合物以及细胞色素C与肌红蛋白、肌红蛋白与溶菌酶混合物及鸡蛋清样品中蛋白成分的成功分离。

Aim:(1) The purpose of this study is to examine whether protein tyrosine kinase and AMPA receptor in the nucleus tractus solitarius and nucleus ambiguus were involved in the regulation of normoxia and hypoxia respiratory response by microinjection of PTK inhibitor and non-NMDA receptor antagonist;(2) To observe the change of number of phosphotyrosine immunopositive neurons in NTS and NA before and after hypoxia using immunohistochemical methods and whether tyrosine phosphorylation and GluR1 in postsynaptic membrane co-localize in NTS and NA neurons.

目的：(1)观察向家兔脑干孤束核或疑核内微量注射酪氨酸蛋白激酶阻断剂和谷氨酸AMPA受体阻断剂对常氧呼吸和低氧呼吸反应的影响，探讨PTK和AMPA受体在低氧呼吸反应中的作用。(2)观察低氧通气后NTS和NA磷酸化酪氨酸免疫阳性神经元的数量变化；观察磷酸化酪氨酸和AMPA受体亚单位GluR1在突触后膜共存的情况，推测在低氧呼吸反应中AMPA受体和PTK信号转导途径的关系。

Western blot was used to examine the phosphorylation of NR2A in rat hippocampus. Results Both Src and Fyn antisense ODNs could decrease the phosphorylation of NR2A induced by cerebral ischemia/reperfusion, while administration of Src antisense ODNs had more suppressive effect on the phosphorylation of NR2A than Fyn antisense ODNs.

对NR2亚基有磷酸化调节作用的是Src家族酪氨酸蛋白激酶（Src family of protein tyrosine kinase， Src PTKs），这是一类膜结合的非受体酪氨酸蛋白激酶；现已知Src家族成员中Src、Fyn、Yes、Lyn、Lck在中枢神经系统中均有表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。