酪状的

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基－4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。

Double fluorescent immunohistochemical staining revealed close apposition of VIP-, PACAP-, and vasopressin-immunoreactive terminals with tyrosine hydroxylase-ir neurons within the dorsomedial arcuate nucleus. We were then of interest to learn from where VIP-, PACAP- and AVP-containing neurons that project to the ARN originate.

经由双重免疫萤光染色法，可以在弓状核的背中区，具酪胺酸羟化酶免疫活性的细胞旁，发现有VIP、PACAP及血管加压素免疫活性的神经末稍的分布及并置，显示确有VIP，PACAP及AVP之神经元投射至弓状核。

In the pretreatment group, Bak Foong Pill extracts were given for 14 days prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相-电化学方法检测纹状体巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

Methods The effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 7-nitroindazole (7-NI) on nitric oxide synthase activity, levels of dopamine、3,4-dihydroxyphenylacetic acid、homovanillic acid, and TH-immunostained postive nerve fibres in the striatum of mice were determined by using colorimetic analysis, HPLC with electrochemical detection, and immunohistochemical analysis.

用比色分析、高效液相色谱电化学及免疫组化法检测1-甲基-4-苯基-四氢吡啶和7-硝基吲唑（7-NI）对C57BL小鼠纹状体一氧化氮合酶活性，多巴胺、二羟基苯乙酸、高香草酸水平和酪氨酸羟化酶免疫阳性神经纤维的影响。

In the pretreatment group, Bak Foong Pill extracts were given for 14 daya prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相－电化学方法检测纹状体多巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

This effect was observed clearly at final concentration between 1 and 10μ mol/L after treating for 24 hours. We concluded that the possible mechanisms of hydroquinone bleaching hyperpigmented skin were through its inhibition of tyrosinase activity at lower concentration and its cytotoxicity at higher one.

提示18α-甘草酸双胺盐、熊果苷的皮肤脱色作用机制与氢醌可能不同，后者低浓度时以抑制酪酸酶活性为主，高浓度时主要是细胞毒，且细胞早期的损伤部位可能发生在树状突。18α-甘草酸双胺盐能否成为一种无细胞毒酪氨酸酶抑制型皮肤脱色剂有待进一步临床加以验证。

METHODS: The spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the SMA for retrograde tracing.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金，观察双侧交感颈上神经节、星状神经节内HRP或FG逆标神经元的分布；采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽Y、钙基因相关肽或P物质。

Methods: the spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the sma for retrograde tracing. the bilateral superior cervical ganglia and stellate ganglia were observed to find retrogradely labeled neurons. the neurotransmitters of these sympathetic fibers around sma were ascertained by using retrograde tracing combined with immunohistochemical double labeled technique.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金，观察双侧交感颈上神经节、星状神经节内hrp或fg逆标神经元的分布；采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽y、钙基因相关肽或p物质。

Furthermore, based on the differences in chloplast DNA, as well as cluster analysis, Machilus and Persea could not be treated as the same genus.

桢楠属和酪梨属仍有些遗传距离，树状图分析结果并不互相隶属，或许可视为相互独立的两个属或是组。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新