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Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠,血清进行肝功能检测;苏木素-伊红染色部分肝组织;Trizol-酚-氯仿一步法提取小鼠肝组织总RNA,紫外分光光度法测定总RNA浓度及纯度,实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达,以18S基因作为质控。

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你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。