配错

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

The purpose of the present invention is to solve the problem of misfit stress in metal base composites.

本发明的目的是为解决已有金属基复合材料存在的热错配应力问题。

The characteristic spectrum could be used to detect the double-bond DNA with one base mispairing.

该特征可被用于检测1个错配碱基的双链DNA。

I won 't forgive myself for that mistake I made , for that little bit of cockiness n .

我不会原谅我犯下的错，因为我的骄傲自大而认为她配不上我。

These data suggest a mechanistic link between DNA mismatch repair and re-methylation at sites affected by methylcytosine deamination.

这些证据显示DNA的G/T错配修复与DNA甲基化谱式的回复之间可能存在偶联关系。

Methylcytosine undergoes spontaneous deamination, at a rate much higher than cytosine, to generate thymine.

甲基化胞嘧啶在生理条件下极易发生自发脱氨，生成胸腺嘧啶，产生G/T错配。

Rare missense mutations were found in the Dicer and Drosha genes, but their presence or absence did not correlate with the level of expression.

Dicer 和Drosha基因检查却几乎没有发现错配的变异，但是无论出现或不出现变异都与表达水平无关。

Look at me now i'm just an empty shell of the man i used to be with you i thought we were right i thought we were perfect but once again my dreams were broken there were no strings attached when i saw you last night i ain't fooling no one oh no - no my love will never die though it's not for you my love will never die it just moves on my love will never die it can break your heart in two my days are so cold and lonely without you but the nights, they really bring me down you left me alone on this cold and winding road on the path that leads to nowhere

不要用翻译机嘛，直译容易错现在，请看着我我只剩下一个人的空壳我一直与你在一起我认为我们是对的我们是绝配但又一次，我的梦想幻灭了没有什么好说了当我昨晚见到你我没有欺骗任何人 oh no no 我的爱永不灭亡虽然它不再属于你我的爱永不灭亡它只是离开了这里我的爱永不灭亡它让你心碎或者。。。让我们没有你的日子如此寒冷和孤独夜晚，让我沮丧你留下我孤独一人在这寒冷蜿蜒的道路没有路标不知方向

It saved them cheap potbs Doubloon interest costs in the short term but it also created a nasty mismatch : their wages and revenues were in one currency and their liabilities were in another .

短期之内这样做会帮助他们省去利息成本，但却创造了一个危险的错配：他们的薪水和收入是由一种货币计算，而债务却是由另一种计算。

By integral transformation of basic equations, the stress and displacement expressions with unknown coefficients of elastic and viscoelastic materials were obtained in Laplace domain respectively, and introducing dislocation density functions, the singular integral equations were got according to the boundary conditions and interface connection conditions, further adopting Gauss integration and Gauss-Jacobi integration formula, the problem was reduced to algebraic equations, then it can be solved with the method of collocation dots in Laplace domain. Finally, the time response of dynamic stress intensity factor was calculated with the inverse Laplace integral transformation.

采用积分变换方法，得到Laplace域内弹性和粘弹性材料的应力和位移的含未知系数的表达式；引入位错密度函数，并通过边界条件和界面连接条件，导出反映裂纹尖端奇异性的奇异积分方程组，采用Gauss积分，并运用Gauss-Jacobi求积公式化奇异积分方程组为代数方程组，利用配点法进行求解；最后经过Laplace逆变换，求得动态应力强度因子的时间响应。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。