配子体

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

As with the male gametes the remaining cytoplasma binding serves the synchronization of the division and maturation of the cells: just as for the 1rst polar body at the time of the termination of the 2nd meiosis.

和雄性配子一样，这些残留的细胞质连接有助于细胞分裂和成熟的同步化：就像第二次减数分裂结束时的第一极体一样。

Fertilization results in triploid or tetraploid individuals.

这是由于染色体在第一次减数分裂的后期没有分离而导致了多倍的而不是单倍体的配子，受精后产生了三倍或四倍体。

Two mode of plastid inheritance, uniparental maternal and biparental inheritance, were discovered in angiosperms at the beginning of this contury, and our knowledge of the mode and cytological basis of cytoplasmic inheritance, esp. plastid, has been improved in many respects following the new cytological and molecular techniques, eg. electron microscopy, DNA fluorescence and RFLP.

近几年由于电镜、DNA的荧光检测及RFLP等新的细胞学和分子生物学技术的发展，使对植物细胞质遗传形式及其细胞学基础有了一定的了解，但多是单一的方法或对雌、雄配子发生、发育个别发育时期研究的结果，对大部分植物种的质体遗传方式或细胞学基础仍不清楚，在有些植物种间应用不同的研究方法得出的结论也不一致。

The effects of different treatments on electrofusion of pig 2 cell embryos were studied with Hubei white pigs as experimental animals.

哺乳动物配子和早期胚胎细胞融合技术在研究卵子和早期胚胎发育中的细胞质相互作用，制作4倍体胚胎，杂种细胞构建和胚胎细胞核移植等方面有重要意义。

PPARα ligands suppressed OPN promoter actiity, and an actiator protein-1 consensus site conferred this repression. Oerexpression of c-Fos and c-Jun reersed the inhibitory effect of PPARα ligands on OPN transcription, and, in chromatin immunoprecipitation assays, PPARα ligands inhibited c-Fos and phospho–c-Jun binding to the OPN promoter.

PPARα配体可抑制OPN启动子活性，一个激活蛋白-1的一致序列位点与这种抑制有关。c-Fos和c-Jun的过表达可逆转 PPARα配体对OPN转录的抑制作用，并且在免疫沉淀实验中，PPARα配体可抑制c-Fos及磷酸化c-Jun与OPN启动子的结合。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

Meanwhile, by either artificial chromosome doubling via colchicine treatment or spontaneous chromosome doubling via a union of unreduced gametes (2n) from T. turgidum-Ae.

同时，通过秋水仙碱对四倍体小麦-节节麦杂种进行染色体人工加倍或利用未减数配子自然加倍，我国科学家创制了一些新的人工合成小麦。

Some stimulators, including viruses, tumor cells and hot shock, could promote the expression of NKG2 receptors and their ligands via activating certain transcription factors which are capable of up-regulating NKG2 promoters' activity. Meanwhile, epigenetic mechanisms including DNA methylation and histone posttranslational modifications are also critical to expression of NKG2 receptors and their ligands and may control the clonally distribution of some NK cell receptors.

病毒、肿瘤和热休克等刺激可以通过激活相应的转录调节因子，提高启动子活性而上调NKG2家族受体及其配体的表达，而启动子区DNA的甲基化状态、组蛋白的乙酰化和甲基化等表观遗传调控，在NK细胞受体及其配体的表达方面亦起重要作用，并决定NK细胞受体的克隆性分布。

Transmission rate of n+1 gametes is an important parameter for genetic analysis of trisomics.

n+1配子传递率是进行三体遗传分析的重要参数。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了