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METHODS: In the rat cardiac hypertrophy model produced by constriction of the abdominal aorta, urotensin II binding sites in myocardial sarcolemma were determined with radioligand assay first day and 30th day after operation.

在腹主动脉缩窄复制的大鼠压力超荷性心肌肥大模型上,采用放射性配基结合技术,测定术后1 d和30 d大鼠心肌浆膜上UII受体的结合位点。

Immobilized metal-chelated affinity chromatography column sorbents matched with Zn2+ were prepared for purifying glutathione using chitosan spherule as substrate.

以谷胱甘肽的酵母抽提液为原料,用自制的以壳聚糖小球为载体、Zn2+为配基的金属螯合亲和层析柱分离纯化谷胱甘肽。

The chapter 6 makes a study on the design and synthesis of new glycosides, which prepared from imidazole, thiazole and tetrazole aglycones mentioned above and glucose derivatives by phase transfer catalysis under the moderate reaction condition.

我们以合成的新杂环化合物作为糖的配基,用相转移催化法合成了25个糖苷类化合物。其中包括4个咪唑氮糖苷,12个含有噻唑环的糖苷,6个含有噻唑环的糖酯,3个四氮唑糖苷。

Here, we review the status of sequence and structure-based approaches to protein function inference and ligand screening that can provide functional insights for a significant fraction of the approximately 50% of ORFs of unassigned function in an average proteome.

这里,我们回顾了序列和基于结构的方式来进行蛋白质功能推断的状况及在一个平均的蛋白质组中非指定功能的约50%的ORFs的重要片段可以提供功能理解的配基屏蔽。

Optimal conditions for receptor binding analysis system were established by unlabeled ligand saturation experiment.

通过非标记配基饱和实验建立理想的受体结合分析系统。

Resibufogenin was administered into the well-grown cell cultures and incubated for 4 d.

把酯蟾毒配基加入生长状态良好的悬浮细胞中转化四天。

Objective: To explore the feasibility of the combination of psuedo-ligand affinitive chromatography and gel chromatography in purifying RIP from bitter gourd and the cytotoxicity of the RIP.

结果: 运用硫酸铵分级沉淀、Blue Sepharose CL-6B假配基亲和层析、Sephadex-G75凝胶层析等方法提取、纯化得到了高纯度的苦瓜RIP,并且能够获得较高的得率;一定作用剂量的苦瓜RIP不仅能够抑制HL-60细胞的增殖活性,还可以诱导其发生凋亡。

Using ammonium sulfate precipitation, Blue Sepharose CL-6B psuedo-ligand affinitive chromatography and Sephadex G-75 gel chromatography, we obtained the typeⅠRIP with high purity, the feasibility of this method has been approved by SDS-PAGE results.

结果显示:1。运用硫酸铵分级沉淀、Blue Sepharose CL-6B假配基亲和层析、Sephadex-G75凝胶层析等方法提取、纯化得到了高纯度的苦瓜RIP,并且本方法提取效率较高,所获得的RIP在SDS-PAGE上呈现为单一条带。

It has been well established that agmatine is an endogenous agonist at imidazoline receptors and noncatecholamine ligand at alpha - 2 adrenergic receptors (α2- AR).

业已证明,胍丁胺是一种内源性的咪唑啉受体激动剂和α2-肾上腺素能受体(α2-AR)的非儿茶酚胺配基

Receptor activator of nuclear factor κB ligand was expressed throughout the chondric lamina of the rat condyle, and was highly expressed in the mature chondrocytes.

核因子κB受体活化因子配基在大鼠髁突软骨的全层均有表达,成熟的软骨细胞表达较强。

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