部分时间的

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Roentgenographic and histologic observation confirmed that bBMP had efficient bone morphogenetic activity.

论文第一部分参照杨连甲的方法从小牛骨基质中提取bBMP，经SDS-PAGE电泳分析，bBMP保留有BMP活性蛋白成分，植入小鼠股部肌肉后的X线及组织学观察发现，所提取的bBMP具有较高的骨诱导活性，诱导成骨的时间和过程与以往的报道一致。bBMP诱导骨形成过程呈现连续又具显著的阶段性，本研究结合组织学和组织化学观察的特点，将其全过程分为四个时期。

Perspective, in all its variations and permutations (e.g., external / internal, divine / human, objective / subjective, impersonal / personal, public / private, singular / multiple, sincere / ironic, omniscient / solipsistic, totalizing / partial), operates as one of the central framing techniques for orienting narrative, inasmuch as it limits what information is available and possible to know at any particular moment as well as how information gets divulged over time.

各种变换的视角（例如：外在／内在，神圣／人类，客观／主观，非个人的／个人的，公开／私有，单一／多数，真诚／讽刺，全知／唯我，整体／部分），为重要的主导叙事的架构技巧之一，在其可限的范围内，讯息的取得、得知的特定契机以及在时间递演下，讯息是如何传播的。

Perspective, in all its variations and permutations (e.g., external / internal, divine / human, objective / subjective, impersonal / personal, public / private, singular / multiple, sincere / ironic, omniscient / solipsistic, totalizing / partial), operates as one of the central framing techniques for orienting narrative, inasmuch as it limits what information is available and possible to know at any particular moment as well as how information gets divulged over time.

各种变换的视角（例如：外在内在，神圣人类，客观主观，非个人的个人的，公开私有，单一多数，真诚讽刺，全知唯我，整体部分），为重要的主导叙事的架构技巧之一，在其可限的范围内，讯息的取得、得知的特定契机以及在时间递演下，讯息是如何传播的。

The original video is spitted into a series of frame groups,for each frame in the group,the points of contour shape are described by MPEG-7,and taking advantage of hash function to calculate the keys between those points in the 1D- DFT transform domain and the watermark signals.

在该算法中，原始视频数据被分割成等帧数的视频组，利用MPEG-7的轮廓编码计算每个视频帧的主要物体轮廓坐标点集合；接着通过傅立叶变换得到该帧轮廓点形状不变量，利用哈希的方法计算水印信号并将这些不变量的有效值作为密钥之一；再对整个视频组的轮廓点进行傅立叶变换，得到其时间轴上的有效不变域，将水印信号嵌入在这个不变域的中频部分以确保了水印的鲁棒性。

In addition, we discovered the law of heat transfer in this susceptor by the analysis of the temperature in it changing with the heating time.

另外，通过对其温度随加热时间变化的分析，发现刻槽基座的热输运规律，即所刻的槽改变了基座中感应产生热量的热传导方向，衬底的热量是由槽上下基座部分传递而来的，且随时间的增大，基座的温度趋于恒定，衬底的温度趋于均匀，这有利于提高生长薄膜的质量。

The power-time curves of E.coli reacting with Co-SG at different temperature has been determined by microcalorimtric method The multiplication rate constant k, generation time G,bacterial growth inhibition ratio I,total thermogenetic quantity Q, the heat quantity of a single bacterium Q_(0) and the heat quantity of a single bacterium per minute have also been calculated according to the power-time curves.

用微量热法测定了大肠杆菌及其在Co -SG配合物作用下的热谱曲线，计算了速率常数k ，传代时间G、抑制I、总产热量Q、单个细菌的产热量Q0 和每分钟单个细菌的产热量 Q0 ，建立了部分代谢参数之间的关系，探讨了大肠杆菌在不同温度和Co -SG作用下的代谢抑制，发现可用t、tr、tg和 Q0 定量表征在不同温度下大肠杆菌的生长代谢和药物的抗菌活性。

Traduction et introduction", reveals Derrida's invention of the important 支柱 of the coming deconstruction theory－the principle of postponement while disclosing Derrida's appropriation and reformation of Husserl's thought through the analysis of "remote communication","the fundamental position of ego","the Verleiblichung of language and writing" and"the seeing and listening of the Ideas .

本部分共以五章的篇幅对德里达的声音解构和时间解构在逻辑上的运作过程进行深耕细作，在行文过程中也充分关注到德里达对胡塞尔符号学理论的误置和阉割之处，但这一部分的重点仍落在德里达在误置中的意外发现上：作为解构理论根基之一的&充替&原理。论文的理论创新

Are International human rights organization, such as "Amnesty International" and "Journalists without Border", only targeting China or are they just being used? Is there any possibility that we could engage a direct dialogue with them to improve relationships? Why has the famous German weekly, the mirror, been issued articles based on unscrutinizing research to smear the image of China? Does this have anything to do with the German government's policies towards China? How should China respond and adjust its foreign policy in turn? Why did some Korean netizens curse so viciously after China's Sichuan earth quake? We had been such big fans of Korean culture, but how do they treat us in return? Why do so many foreigners still believe that Chinese still wear robes and have queues? How to let more foreigners know what modern China really like? when some of our compatriots leave the country and behavior inappropriately, were these details responsible for our negative impressions by some foreigners or did they weight more on it? Look before you leap.

像&大赦国际&&记者无疆界&这样的国际人权组织是不是只针对中国，他们是不是被利用，我们有没有可能与他们直接对话传递信息改善关系；德国著名的《明镜》周刊为什么近段时间频发不基于严谨事实调查而有损于中国形象的封面文章，这和德国执政者的对华政策有何关系，中国在外交上如何应对和调整；韩国部分网民为什么对中国的四川大地震有如此狠毒的诅咒，我们曾如此&哈韩&，而韩国人对中国到底是怎样的态度；为什么还有那么多外国人认为中国人还是穿袍子留辫子的，怎样让更多的老外了解真实的当代中国；某些国人出国期间表现出不恰当的行为举止时，这些细节是不是也是某些外国人对中国负面印象的缘起或加重码……请三思而后行。

For each party to get what it wants, several bets will have to come good over the next five years or so. The equities in the pension scheme will have to outperform government bonds by a decent margin. Global will have to break a decade-long habit of losing money. BT will, for the first time in a generation, have to produce industry-leading new products. And a unionised workforce will have to shrink without employees being offered big financial inducements to leave.

他们要想从公司能获得他们所期待的，只能对公司的未来下赌注——未来5年BT能从困境走出：退休金计划中的股票部分，将获得比政府公债多得多的收入；Global也将一改十多年的气象，扭亏为盈；BT将在一代人的时间内生产出业界领先的新产品；随着雇员被巨大的诱惑吸引主动离职，公会的力量也将被削弱。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失