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In this paper, RAPD, ISSR, and SSR reaction system of the fruit tree were established, and the genetic diversity of 56 genotypes of Japanese plum were analyzed using RAPD, ISSR, and SSR markers. The optimal reaction of RAPD was studied with 5"-GACCGCTTGT-3" primer for cv. Fenghuali.

本文选择了56个中国李品种资源(包括2个欧洲李品种),从叶片基因组DNA提取方法入手,在建立和优化中国李RAPD、ISSR和SSR反应分析体系的基础上,利用这三种分子标记对中国李品种资源进行聚类分析,旨在促进中国李品种资源分类和遗传育种研究。

In this study we mapped a grain length controlling gene named Lk-4 with SSR and CAPs markers by screening 800 recessive plants in a BC2F2 population derived from a cross of Shuhui527×Xiaoli and backcrossed with Xiaoli as the donor parent.

对F2和BC2F2 群体籽粒大小形状和千粒重的遗传分析表明,回交能将大部分对目的基因效应具有干扰修饰作用的微效基因多态性除去,从而有利于对目的基因型的准确鉴定;在F2和BC2F2群体中只发现两类籽粒长短表现型,即短粒和长粒,并且二者分离比例符合3:1的典型一对等位基因分离比例。

However such understanding can never embrace all that soul, angels and divas understand about genetic information and formation of form.

然而这类认知永远无法融合所有灵魂、天使和提婆对遗传信息和形体构成所理解的全部知识。

Most of the triazole fungicides are widely used against rusts, powdery mildews and scabs. But triazole fungicides were harmful to fish and other aquatic organisms. And the Environmental Protection Agency has classified TBZ as a "possible human carcinogen" because it caused significant toxicity and reproductive effects on laboratory mice.

但是三唑类杀菌剂对于鱼类和其他水生生物有害,而且由于戊唑醇对老鼠具有毒性和遗传效应,美国环境保护署(Environemt Protection Agency, EPA)把戊唑醇列为&人类可能致癌物质&。

The NPMOGA algorithm based on non-penalty parameter constraints solving method is composed of the niched Pareto genetic algorithm and non-penalty parameter constraints solving method.

基于无罚系数约束处理方法的NPMOGA算法,由基于Pareto竞争和等价类共享的小生境Pareto遗传算法和无罚系数的约束处理方法组成。

In this study, 105 TDFs were in silico mapped in the rice high-density linkage map. Nineteen TDFs were mapped to the quantitative trait loci regions for root growth under water-limited conditions in, at least, two of three rice populations derived from three crosses with the same parent of Azucena (Bala×Azucena, IR64×Azucena and IR1552×Azucena). Four of 19 genes (T37, L16, T17 and T7) were mapped based a RIL population of IR1552×Azucena by southern blot analysis. Five genes encode putative or hypothetical protein. Other 14 genes were similar with known genes in databases including expansin (OsEXP2), late embryogenesis-abundant gene , an SR-related protein essential for spliceosome assembly (SART1), autophagocytosis protein , bHLH protein, fruit-ripening protein similar to ASR, nickel-binding protein 2A, DNA-binding protein, pyruvate dehydrogenase kinase , stomatin-like protein, SR1 induced by sucrose starvation, vacuolar protein sorting protein (VSP33a), gibberellin action negative regulator and retroelement.

根据核苷酸序列将105个基因电子定位到水稻的高密度连锁图谱上,其中19个差异表达基因定位在Bala×Azucena、IR64×Azucena和IR1552×Azucena中至少两个群体共同的与根生长相关的QTLs区间,并用Southern杂交将其中的4个定位到IR1552×Azucena群体遗传连锁图谱相应的位点上。19个基因中的5个编码推断的未知功能蛋白质,其余14个编码已知功能蛋白,分别为膨胀素(Os-EXP2)、胚胎后期丰富蛋白、剪接体安装必需的SR相关蛋白(SART1)、自吞噬蛋白、碱性螺旋-环-螺旋转录因子、与ASR相似的果实成熟蛋白、镍结合蛋白、DNA结合蛋白、丙酮酸脱氢酶激酶、stomatins类蛋白、蔗糖调节蛋白SR1、液泡蛋白分类蛋白(VSP33a)、GA负调节因子、逆转座元件。

Aimed at the arising problems such as the cross-incompatibility and hybrid's sterility during distant hybridization of fruit trees, effects of different treatments on cross-compatibility of distant hybridization in stone fruits were studied based on the former studies, then physiological and biochemical changes during hybridization between plum and apricot were primarily discussed, and rescue and culture system of hybrids' embryos were also conducted, further the genetic variation and principle between the hybrids and their parents were analyzed and methods of identification of hybrids were established.

针对果树远缘杂交过程中存在的杂交不亲和性及杂种不育性等问题,在前人研究的基础上,研究了不同处理对核果类果树远缘杂交亲和性的效应,并探讨了李、杏属间杂交过程中发生的生理生化变化以及花粉与柱头的互作,建立了李、杏属间远缘杂种的胚培养技术体系,建立了杂种鉴定检测方法,并分析了杂种后代与其父母本之间的遗传变异及规律,最终将一批远缘杂交新种质定植于大田,同时对自然界存在的杏梅种质资源进行了RAPD分析。

The plum-apricot germplasm resources were analyzed by RAPD markers. The results showed that plum-apricot was more close to the related species with low polymorphism. The clustering results were indicated that most plum-apricots were more close to Chinese plum with the similarity of morphological description, thus the female parent for plum-apricot was probably plum in the occurrence naturally. The result of S allele-specific PCR amplification showed that plum-apricot had only one band with the similarity with plum, thus evolution similarity of plum-apricot was more close to plum than apricot.

5对参试杏梅种质资源的RAPD分析表明,杏梅与其近缘种之间亲缘关系较近,多态性相对较少;聚类分析结果显示大部分的杏梅与中国李的遗传距离较近,与形态学上的描述基本一致,因此杏梅种质资源在自然界中产生时,其母本可能为李;同时S等位基因的扩增结果显示杏梅与李的扩增条数一致,都为一条,因此推断杏梅与李的进化趋同性可能较杏更近。

First, use OTSU method to make license plate image binarization, and then use genetic algorithm to map the entire license plate matching search feature. With a fitness function formed by the area character vector, the optimal locating parameter of the plate area was found.

先用最大类间方差法对车牌图像进行二值化,然后利用遗传算法对全图进行车牌特征匹配搜索,结合区域特征向量构造的适应度函数,最终找到车牌区域的最佳定位参量。

It is not only a safe and socially friendly form of sport for both you and your dog to enjoy, but the training and trial preparations also provide valuable information to the breeder about the dog's key inheritable instincts and general character framed within a formal test.

这不仅仅是一项安全的,社会化的,友好的,而且你和你的狗都能够乐在其中的运动形式,而且这些训练和考验的准备给繁育者们提供了非常有价值的信息——在这场正式的测试里,犬类的本能的遗传和综合的性格构成。

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