遗传类

As investigated, the shape of the fruit was mainly rotundity, and the color of the fruit were red, black, yellow, mauve. Variation coefficient of mineral elements, which revealed wider variation range and rich genetic diversity. Among them, Fe, the content and the variation coefficient of which was the highest(2.12 mg·100g-1 and 26.99％), it has large potential for futher selection. Ripe fruit of 12 wild myrobalan plum seedlings were analyzed using head space-solid phase microextraction and gas chromatography-mass spectrometry. The results showed considerable genetic variations in these aspects: The total content of volatile components, the classes and contents of each compounds classes, the segregation ratio, and content of main components. There were 83 compounds in total belonging to 6 classes detected in 12 wild myrobalan plum seedlings, including esters, alcohols, ketones, aldehydes, heterocycle and Hydrocarbons. Among them, Formic acid, hexyl ester, the content of which was the highest(4.33 μg·g-1),was important character impact odors.

结果表明，①新疆野生樱桃李实生株系的果实形状主要为圆形，少数为椭圆形、卵圆形、卵形和宽卵形，果实颜色有黄色、红色、紫红色和黑色等4种类型，野生樱桃李果实纵径、横径、单果重、果形指数以及可溶性固形物含量均存在一定程度的变异，遗传多样性较为丰富，其中以单果重的变异系数最大(9.13 %)；②新疆野生樱桃李实生株系果肉组织的Zn、Ca、Mg、Mn、Fe及Cu等6种矿质元素含量变异丰富，其中以Fe元素的含量（平均值为2.12 mg·100g-1）和变异系数（26.99％）最高，进一步筛选高铁性状单株的潜力很大；③从新疆野生樱桃李12个实生株系中共鉴定出醇类、醛类、酯类、酮类、烃类及杂环类等6类83种挥发性化合物，各实生株系挥发性化合物总含量、挥发性化合物种类及其含量以及主要挥发性化合物分离比率与含量等存在广泛的遗传变异，遗传多样性较为丰富，其中以甲酸己酯含量最高（平均值为4.33 μg·g-1），是野生樱桃李果实香气形成的重要特征性化合物。

The results showed that the average heterozygosity was higher in 9 populations,the lowest is Jinding, the highest is San sheldrake, which ranged from 0.5137~0.6055. The average heterozygosity of 9 duck populations was 0.5523, which reflected the rich diversity. Considerable breed differentiation was observed and 25.65% of the total genetic variation came from breed differences, this result affirmed each breed was of own genetic diversity. The DA genetic distances suggested the longer differentiation existed between those breeds. The domestic duck breeds in East China were clustered into four groups based on the NJ clustering, the clustering results had some relationship with the distributions and economic utilizations of these duck breeds.

结果表明：9个品种的平均杂合度都较高，最低的为金定鸭，最高的为山麻鸭，杂合度范围为0.5137～0.6055，群体平均杂合度为0.5523，反映了各鸭种的杂合度都较高，遗传多样性丰富；华东区各鸭种间存在较大的遗传分化，25.65%的遗传变异来源于品种间的差异，更进一步反映了各品种具有本品种特征特性的多样性；通过计算DA遗传距离发现各群体的遗传距离较远，分化时间较长；NJ聚类结果将华东区家鸭资源聚为4类，NJ的聚类结果分析与几个鸭品种的地域分布和经济用途有一定关系。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析，分别提取19份马铃薯品种的DNA，进行随机引物多态性扩增，从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究，每条RAPD引物扩增出4~9条带，共获得52条带，其中多态性条带为43条；19份马铃薯品种的遗传距离介于0.17~0.72之间，平均值为0.39，平均遗传距离介于0.31~0.51之间；聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类，聚类结果与系谱分析基本相符，同时也说明克新系列马铃薯的遗传基础有所拓宽。

Sickle cell disease, also called sickle cell anemia, is a genetic condition that deforms red blood cells.

镰状细胞病也叫做镰刀性细胞贫血病，是红细胞残缺的一种基因遗传类疾病。

The results above showed:(1) The cultivars of large-flowered chrysanthemum are widely distributed in Beijing area with plentiful floral types and floral colors;(2) The cultivars with the rare types and colors are urgent for protection;(3) Most of the quantitative characters obeys to the normal distribution,some important traits agreed with skewed distribution.The probability grading can assist quantitative character in establishing new grading standards;(4) Most of qualitative characters is asymmetrical in cultivars,which might be caused by unbalanced differentiation of characters or linkages coming from emphasis characters selection;(5) The floral characters are relatively stable and have comparably large influence on classification,the characters of stipule also have a certain degree of stability and can act as an reasonable supplement for cultivars classification,but the foliar characters have worse stability and can only be recognized as lower level criterion.(6) The genetic relationship among the Flat-petaled group,the Quilled-petal group and the Spoon-petaled group are closest,which followed by the Filiform-petal group because of long-term selection pressure for the specialization of the petal tip loved by most people.The Anemone-petal group has further genetic relationship with these four groups.(7) ISSR molecular marker detection indicates that the proportion of polymorphic loci of lardge-flowered chrysanthemum is higher,in which the Flat-petaled group has the highest genetic diversity level.

综合以上的研究结果表明：(1)北京地区现有大量大菊品种，且花型与花色较为丰富；(2)珍稀花型、花色品种亟需保护；(3)大部分数量性状是符合正态分布的，少数重点观赏性状迫于选择压力呈现偏态分布，概率分级法能够辅助大菊的数量性状建立新的分级标准；(4)大部分质量性状在品种群内的分布是不均匀的，可能是由菊花性状遗传分化的不均衡性或受到选育重点性状的连锁作用而造成的；(5)大菊花部性状相对稳定，对品种分类影响较大，其托叶部分也具有一定程度的稳定性，可以作为品种分类的合理补充，而叶部性状稳定性较差，在品种分类中应作为更次一级的分类依据；(6)平瓣类、管瓣类和匙瓣类的亲缘关系最近，畸瓣类品种花瓣先端特化受到人们的喜爱，长期的选择压力使其区别于3种基本瓣型，而这四种瓣型与桂瓣类在亲缘关系上较远；(7)ISSR分子标记检测表明：大菊品种多态性位点百分率较高，平瓣类品种的遗传分化最为丰富。

The 54 accessions were classified into five major groups:Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ by cluster analysis using UPGMA, which showed significant relationship with the origin regions of accessions. Genetic differentiation between and within eight eco-geographical groups of C.dactylon was estimated by Shannon's diversity index, which showed that 63.81% genetic variance existed within group, and 36.19 % genetic variance was among groups, indicating rather large genetic distances among those germplasm, and there was a correlation between genetic differentiation and eco-geographical habits among the groups.

聚类分析结果表明，52份材料可聚为5类；基于Shannon多样性指数估算了8个狗牙根生态地理类群内和类群间的遗传分化，类群内的遗传变异占总变异的63.81%，类群间的遗传变异占总变异的36.19%，表明这些抗源材料遗传差异较大，各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性。

The experiment showed: 1PER, EST and AMY were polymorphic, meanwhile the isozymic chromatograph could identify the cultivars in this study; 2Two multifoliolate cultivars had specific isozyme loci PER-4, and the level of PER activity of the internal cultivars was higher than the external cultivars; 3 Genetic similarity and euclidean coefficient between the cultivars were 0.826-0.952, 0.500-1.118, between the multifoliolate cultivars were 0.904, 0.500; 4 Multifoliolate cultivares were classified for one type, and others for two other types.

结果表明：1测定的5种同工酶中过氧化物酶、酯酶、淀粉酶具有多态性，综合此3种酶酶谱可以鉴定供试的7个品种；22个多叶型品种具有一共同特征酶带PER-4，国内品种的过氧化物酶活性明显高于国外品种；3品种间的遗传一致度为0.826～0.952，欧氏遗传距离为0.500～1.118，多叶型品种间遗传一致度为0.904，欧氏遗传距离为0.500；4等级聚类分析结果为，以欧氏遗传距离0.400为分界，供试品种分为3类，2个多叶型品种为一类，其余的5种三叶型品种分为另两类。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Firstly,the author analyzes principles and general methods of lustering- based Data mining.

本文作者首先认真研究分析了基于聚类的数据挖掘的基本原理，提出了一个将单亲遗传算法与Kohonen聚类算法相结合的混合聚类算法，克服了Kohonen 算法的局部最优问题以及采用普通遗传算法聚类时的搜索速度和聚类精度的矛盾。

The 25 acces-sions were classified into three major groups: A, B, and C by cluster analysis using UPGMA, which showed significant relationship with the origin regions of accessions. Thus, it was suggested that the Sichuan hulless barleys could be used as elite germplasms to enhance the genetic background for super-hulless barley breeding.

聚类分析结果表明， 25份材料可分成A、B、C 3大类，材料聚类与其来源地有明显的相关性。25份材料间的平均遗传距离较小(0.3240)，平均遗传多样性较低(0.5126)，遗传基础较为狭窄。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了