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Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料,分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异,两种生境鸭跖草在铜胁迫下养分吸收和积累的差异,两种生境鸭跖草在铜胁迫下生理反应的差异,以及铜在鸭跖草细胞内的分布及结合形态研究,矿山鸭跖草差异表达的cDNA克隆,铜对鸭跖草种群遗传分化的影响,从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

In this study, we investigated the possible regulatory mechanisms and roles of epigenetic modifications in HOXB13 gene transcription.

本文探讨了表观遗传修饰对HOXB13基因转录的影响,并且对其可能的机制和意义做了深入的研究。

This view seems to require revision in views of more recent experimental finding that both relay cells in the lateral geniculate nucleus and the ganglion cells in the retina do show some orientation and direction sensitivities.

近年来大量的实验结果表明,视皮层下的外膝体神经元和视网膜神经节细胞都具一定程度的方位和方向敏感性,这些性质是遗传决定的,不受后天环境的影响。

However, the mechanism inducing agonistic behavior is not clear until now. We used 24 colonies of C. formosanus from Hunan, Hubei, Guangxi, Anhui, and Zhejiang provinces to study the relations between the inter-colony agonistic behavior and the greeting behavior, or the secretion of frontal gland, gnathal gland and abdominal gland, the compounds of body wall, the genetic background, the contents of microelement and protein in body, the food kinds, the microorgansim in intestinal cavity, the season change, the colony age, and the colony individuals quantity.

我们以采自湖南、湖北、广西、安徽、浙江等省的24巢台湾乳白蚁为材料,对其问候行为、额腺、颚腺和腹腺分泌物组成、表皮化合物组成、遗传背景、体内微量元素和蛋白质含量、食物种类、肠道微生物等与格斗行为的关系及季节变化和巢龄、巢群大小对格斗行为的影响作了大量的研究。

Surveys of their cocoon and filament quality show variations compared with control plot, found a mutant that has hereditable character. All those indicated that He-Ne laser has distinct inductive effects on mulberry wild silkworm.

丝质调查与对照相比也有差异,产生显著差异的个体,能继代遗传,显示He--Ne激光辐照对野桑蚕生理生化性状有明显影响。

Not only it impairs patients" genital health and Quality of life, but also is associated with an increased risk of type 2 diabetes, endometrial carcinoma, hyperlipemia, hyperpiesia, cardiovascular and cerebrovascular events. Up to now, precise pathogenesy of PCOS is yet unknown. The modern medicine indicates the etiopathogenisis of PCOS related to hereditism, endocrinology, immunology, metabolism and so on. The main features are hyperandrogenism and hyperinsulinemia, the correlation and between the above factors and how they inference PCOS are still unclear. In the view point of Traditional Chinese Medicine ,PCOS classifies to "menopathy","infertility" and "Zhengjia , its occurrence exists a close relationship with dysfunction of liver, kidney, spleen and further inducing deficiency, sputum, sluggish stagnant and fire. Many scholars insist that pathogenesis of PCOS is a renal deficiency plus phlegmatic hygrosis. However, after many years clinical observation.

随着研究的不断深入,PCOS日益显示出其复杂性和重要性;他不仅影响到患者的生殖健康及生存质量,而且其异常的激素环境使该类患者对子宫内膜癌、高血压、高血脂及心脑血管意外等病发生危险明显增加;但目前该病的确切发病机制尚不十分清楚,现代医学认为其病因涉及到遗传、内分泌、免疫、代谢等多个方面,以高雄激素血症与高胰岛素血症为主要特征,二者在PCOS发病中的确切作用,目前尚不清楚;PCOS相当于祖国医学的&月经病&&不孕&&癥瘕&等范畴,本病的发生与肝、肾、脾功能失调导致虚、痰、瘀、火有关。

The homeotic gene not only plays a role in the tegulation of cell ar- rangement in plant s organ, but also is the base of the floral meristem identity, inflores- cence meristem identity and floral organ identity.

植物由营养状态转入生殖状态是发育过程中的重要一步,其转变过程受遗传固子及环境的双重控制,光周期与温度是影响开花诱导的主要环境固子,而植物的同源异型基因参与植物器官内细胞排列方式形成的调拉过程,是拉制花分生组织特异性、花序分生组织特异性和花器官特异性的基础。

And a new study shows that genetics may be a factor: inherited traits, such as impulsivity, can make a person more or less willing to have sex at an earlier age.

另外最近的研究表明基因也许是一个决定&破处&早晚的因素:在遗传性状中,如是否易冲动,会影响到一个人是否会在较小的年龄时就发生性行为。

To obtain a better understanding of the historical dynamics of present-day species in this region, we examined the population structure of chloroplast trnT-trnF sequence variation in Juniperus przewalskii, an endemic tree species mainly distributed in the northeast plateau.

由于青藏高原的地貌效应,第四纪冰期气候的反复变化应对现今该地区生物的地理分布及其居群遗传结构产生重大影响。

Especially,it is greatly affected by temperature,humidity and other treatments during malting.

它不但受大麦品种本身遗传因素和环境条件的控制,制麦期间的温、湿度以及其它各种理化处理对其也有很大影响。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。