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In this study we mapped a grain length controlling gene named Lk-4 with SSR and CAPs markers by screening 800 recessive plants in a BC2F2 population derived from a cross of Shuhui527×Xiaoli and backcrossed with Xiaoli as the donor parent.

对F2和BC2F2 群体籽粒大小形状和千粒重的遗传分析表明,回交能将大部分对目的基因效应具有干扰修饰作用的微效基因多态性除去,从而有利于对目的基因型的准确鉴定;在F2和BC2F2群体中只发现两类籽粒长短表现型,即短粒和长粒,并且二者分离比例符合3:1的典型一对等位基因分离比例。

By PCR analysis using specific primers, one specific band at 600~700 bp was distinctively present in cms backcrossed generations. It was genetically stable and cytoplasmically inheritable in the various backcrossed progenies.

根据Brassica nap型细胞质雄性不育相关基因orf222设计兼并引物,利用特异引物PCR方法,分别在不育源、F1、BC2以及BC3世代中扩增出一条600~700 bp 大小的特异条带,而在叶用芥菜保持系中无此条带,具细胞质基因遗传特性。

In this study, genetic analyses were conducted to determine the genetic basis in an elite resistant inbred line Siyi with complete resistance to maize dwarf mosaic. A new genetic model, two dominant complementary genes conditioning the resistance, were found by Mendelian genetic analysis based on parents, F1, F2 and backcrosses in three successive years' field trails. The two genes were further mapped near the centromere of chromosome 3 and 6, respectively by tightly linked microsatellite markers using 242 plants from F2 generation. The resistance gene on chromosome 3 is 1.0 cM apart from the flanking markers phi053 and umc1527, respectively. Whereas the linkage distance between two flanking markers bnlg1600 and phi075 and resistance gene on chromosome 6 was 1.0 and 4.0 cM, respectively. Genotypic analysis of the plants from testcross and F3 populations supported the new genetic manners.

课题组通过连续三年的抗病鉴定,在国内种质资源中筛选出一份综合农艺性状优良、配合力较高的自交系四一,三年的表型遗传研究和两年的分子标记工作,发现四一中的玉米矮花叶病抗性是由两对显性互补基因控制的,进而利用F2作图群体,把发现的两个基因定位在第三和第六染色体的着丝点附近,并获得了双侧紧密连锁的分子标记连锁图谱,其中第三染色体上的分子标记UMC1527和phi053从抗病基因双侧逼近1 cM,而第六染色体上的分子标记phi075、bnlg1600从抗病基因双侧分别逼近4 cM和1 cM;利用B2群体、F2:3家系、BC3F1群体和带有第三、第六染色体抗病基因以及两个抗病基因的近等基因系,进一步证实了四一中成株期抗性是由两个显性互补基因控制的。

Farther, the sperm DNA of wild male was extracted from copulated wild female thelycum in three families of the ten.

从其中3个家系的雌性亲虾纳精囊中提取了雄虾精子DNA,微卫星标记显示各家系的子代个体均有一个等位基因与雄性亲虾的基因型相符,且符合孟德尔遗传规律,这为野生中国明对虾雌虾在繁殖季节一对一的繁殖行为提供了遗传学的证据。

In this family, five F1 males and twenty-three F1 females were intercrossed to generate 147 F2 offspring.

根据美国肉畜研究中心公布的猪遗传连锁图谱,在1号和3号染色体上等间隔(20cM)选择8个和9个微卫星标记,对参考家系全部的F~0|、F~1|和F~2|个体进行扩增,获得各标记位点基因型。

Genetic susceptibility modulates the impact of obesity on risk for type 2 diabetes.

遗传易感性调节着肥胖者患2型糖尿病的可能性大小。

Background— Protein kinase A signaling has long been known to play an important role in cardiac function. Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal features. Mouse models of this genetic defect have been unsatisfactory because homozygote null animals die early in development and heterozygotes do not exhibit a cardiac phenotype.

背景——蛋白激酶A信号传导在心功能中发挥了重要作用早已为人们所知蛋白激酶A调节亚基因PRKAR1A的变化引起蛋白激酶A系统失调,导致了遗传性卡尼复合肿瘤综合症,其中包括的心脏粘液瘤作为它的一个主要特征此种遗传缺陷的小鼠模式仍然不能令人满意,因其纯合体空白动物在发展中早期死亡,而杂合体不能显示心脏表型

On the basis of that all frame offsets are given, a genetic algorithm is presented to optimize the object.

在建立全船肋骨型值的基础上,提出了改进的遗传算法来优化货舱区分段环焊缝位置方法。

The reason is that the c2 distance measurement in correspondent analysis usually overrates the effect of the genes with low frequencies.

蹄型效应经常会歪曲潜在遗传结构的真实形态,其产生主要是因为对应分析中的c2距离不相似测度高估了稀有基因的作用。

Objective: Type 2 diabetes is heterogeneous in its clinical features, pathogenesis, and predisposing or causal genetic factors.

目的:2型糖尿病在临床表现、发病机制和遗传易感或致病因素上各不相同。

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