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Erianthus arundinaceum; Sugarcane; ITS identify; Karyotype analysis

斑茅;甘蔗;ITS鉴定;核型分析;染色体遗传行为

Existing plasmids and inherited materials are used to construct expression vector pZMW which could be integrated into S. erythraea chromosome and multi-copy expression vector pZM.

本实验室利用现有的质粒和遗传材料,构建了糖多孢红霉菌染色体整合型表达载体pZMW和游离型多拷贝表达载体pZM。

The strongest evidence for linkage in 147 families with late-onset PD was on chromosome 17 near the tau gene -- previously shown to be involved in other neurodegenerative diseases including frontotemporal dementia with parkinsonism and progressive supranuclear palsy.

147个患有迟发型帕金森氏症的家庭在遗传研究中被发现,该病与17号染色体上的tau基因有关联。以前tau基因被认为与其它的神经变性疾病有关,如伴有震颤麻痹的额颞型痴呆以及进行性核上性麻痹有关。

Finally, the HIV-1 genotypic assay was used to study polymorphisms in the HIV-1 Gag p6 region.

最后,应用基因型耐药性分析方法对Gag p6蛋白的遗传多态性进行了研究。

The distribution of genotypic composition was normal.The population is ideal for gentic mapping and other genomic research

该RILs群体各家系的基因型组成近似正态分布,是一个适于遗传作图及其他基因组研究理想的RILs群体。

The data byχ~2 were consistent with the separation ratio as 1: 2: 1 χ~2=4.028<χ_(0.05,2~2=5.99, which further conformed that glandless character was controlled by a incomplete dominance gene, Gl_2~e. 2. SSR markers every about 5cM were selected in the chromosome 12 of our lab genetic linkage map to screen the parent plants. The markers with the polymorphism were used to amplify the DNA of 210 F_2 segregating plants selected randomly and recorded the marker genotypes. After eliminating the segregation distortion markers byχ~2 test, other SSR markers were detected by linkage analysis.

二、参照本实验室海陆种间遗传连锁图谱第12染色体上的SSR标记,平均每5cM左右选择一个标记进行亲本多态性的筛选,筛选出具有多态性的标记位点进一步扩增随机抽取的210个F_2群体单株的DNA,鉴定每个个体的标记基因型。X~2检测剔除偏分离标记后进行连锁分析,发现Gl_2~e基因位于CIR362与NAU5079之间,进一步参考图谱上位于CIR362与NAU5079之间的标记加密图谱,最终将其定位在CIR362和NAU2251b、NAU3860b、STV033之间。

Glyoxalase I Genetic Polymorphism Gene frequencies Phenotype

乙二醛酶;遗传多态性;基因频率;表型

In this paper, 52 transgenic insect-resistant cotton varieties of Gossypium hirsutum from China and abroad, have been used to investigate fiber quality traits, construct cluster dengrogram. Based on the SSR and AFLP analysis, we analysised the diversity and constructed the fingerprinting of the cultivars.

本研究选取国内外陆地棉转基因抗虫棉品种52份,对其纤维品质进行测定研究,建立聚类图;并利用SSR和AFLP分子标记建立52份供试材料的指纹图谱,从表型和分子水平综合分析其遗传多样性。

Was used to cons Methods: The pSUPER plasmid cloned into RNA polymeraseⅢ H1 promoter was used to construct new vectors which can code hereditable shRNA aimed to HPV18E6/E7 gene mRNA.

采用克隆有人Ⅲ型RNA聚合酶H1启动子的pSUPER质粒,设计并构建针对HPV18E6、E7基因mRNA的、可稳定遗传的编码发夹状结构的特异性RNA干扰质粒载体。

1Unequal number of univalents with decreased degree of synapsis and lagging chromosomes occurred in meiosis.The abnormal chromosomal behavior correlated with heterogenicity gave rise to unequal allocation of homogenous chromosomes to daughter cells and resulted in the deletion of functional chromosoms,thus led to the occurence of a certion proportion of aborted pollens.(2)The interaction between genetic imbalance and such environmental factors as temperature,etc.,further drew forth physiological and even structrural imbalance,with abnormal development of pollen mother cells and tapetal cells,and consequently resulted in no or very few production of pollens.The difference in the interaction between environment and genotype reflected on the unstability of pollen abortion in different years.

1在减数分裂中有数目不等的联合程度较差的单价体以及落后染色体出现,这种与异质性相关的染色体行为异常,导致同源染色体向子细胞的不均衡分配,造成具功能染色体的缺失,从而引起毛白杨一定比率的败育花粉的产生;(2)遗传上的不平衡与温度等环境因子相互作用,进一步引发毛白杨生理乃至结构上的不平衡,花粉母细胞和绒毡层细胞发育异常,从而造成不产粉或产粉较少;环境与基因型互作的差异性导致了花粉败育的年度不稳定性。

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