造血的

Objective: To explore the effect of ABO mismatch on clinical characteristics in allogeneic hematopoietic stem cell transplantation.

目的：分析ABO血型不合对异基因造血干细胞移植的影响。

Can accelerate the bone marrow cell from G1 phase into S phase when we measure it's Cell cycle, it make S phase rate was increased .thus it promoted DNA synthesize and repaired. Mice irradiated at dose of 5.5 Gy gamma-irradiation showed timed-related decreases and restores in peripheral blood picture at day 1 through day 21, the counts of WBC of these group had taken drug has various degree to increase than irradiation control group in day 1. Grugs failed to protected the peripheral blood cell at lowest point, but seems promote their rescovery.9803 treated group was distinct. At 7days after mice were radiated in 3.5 Gy. we observed drug can Significantly hold back the decreased number of hematopoietic progenitors colony forming radiation induced. These findings indicated 9803 have a certain radioprotective activity against gammer irradiation in mice.

给药组的 CFU-S 数量较照射对照组明显增加，3.5Gy 照后第七天观察给药9803组对小鼠骨髓造血组细胞集落生成能力的影响，给药组的造血祖细胞集落的形成能力强于照射对照组，并且9803组明显优于523组。5.5Gy 照射引起的小鼠的外周血细胞数量的降低以 WBC 发生最早，照后24小时给药组的 WBC 数量均比照射对照组有不同程度的升高，以9803各给药组最为明显，药物未能使照射引起的外周血细胞最低值升高，而有降低的趋势，但给药对照射后期外周血象的总体恢复有较好的促进作用。7.5Gy 照射后第七天测定小鼠骨髓细胞周期的测定中发现9803具有促进骨髓细胞由 G1期进入 S 期的效应，致使 G0/G1期细胞显著减少， S 期细胞比率显著增加，这利于促进 DNA 合成修复，即促进骨髓细胞增殖，骨髓细胞 DNA 含量给药组显著高于照射对照组。

We show that the HSC compartment of PV and PMF patients contains JAK2V617F-positive long-term, multipotent and self-renewing cells.

研究结果发现PV和PMF小鼠的造血干细胞中获得了长期植入的JAK2 V617F阳性的能自我更新的多能造血干细胞。

There is no mature scheme on haploidentical non-T-depleted transplantation in China. Prognosis of chemotherapy is poor for refractory and high-risk leukemia. Allogene hematopoietic stem cell transplantation should be applied without delay. If we can go beyond the histocompatibility leukocyte antigen boundary, HLA-haploidentical family members would became the resource of allo-HSCT donors.

目前，国内外尚无单倍型相合未去T细胞移植成熟的方案，对于难治和高危白血病患者，化疗预后是很差的，应不失时机进行异基因造血干细胞移植，如果能够跨越人类主要组织相容性抗原的限制，用人类主要组织相容性抗原半相合的亲属作为造血干细胞移植的供体，就可以彻底解决干细胞来源问题。

The transforming growth factor-β superfamily, a large group of highly conserved growth factors including TGF-βs, activins and BMPs, regulate a wide variety of cellular functions such as proliferation, differentiation, apoptosis and migration. Signals from these growth factors are transduced by a group of Smad proteins. To date, there are nine vertebrate Smads, including the receptor-activated Smads , Smadsl-3, 5 and 8, the common mediator Smad4 and Smad4β, and the inhibitory Smads, Smad6 and 7. Signaling is initiated when the ligand induces assembly of a heteromeric complex of type Ⅱ and type Ⅰ TGFβ receptors. Then R-Smads are directly phosphorylated by activated type I TGF-β receptors.

在多种SMADS中，SMAD5令我们产生兴趣，基于三个原因：1、Smad5基因敲除胚胎中发现卵黄囊的异位造血以及CFU-GM祖细胞数目增加，表明SMAD5可能是早期造血发育的负向调控基因。；2、应用反义核酸封闭Smad5可以逆转TGF-β对祖细胞增殖的抑制效应，表明它可能介导TGF-β信号转导，后者的异常，包括某些SMADS的功能异常，和白血病紧密相关；3、Smad5基因定位在人染色体5q21，此区域的缺失和急性髓系白血病和骨髓增生异常综合症相关，因此，Smad5被怀疑为白血病抑制基因。

The 1〓 generation of which is transfusion with mature cells, such as the whole blood and blood ingredient: the representative of the second generation is hematopoietic stem/progenitor cells transplantation, which can regraft normal hematopoiesis and immunity in a body with destroyed immunity and hematopoiesis by the self-renewal and multiple differentiation capacity of stem cells.

第一代细胞疗法是成熟血细胞的输注，如全血输注及成分输血等，对于纠正各种血细胞减少有明显疗效；第二代细胞疗法的代表是干细胞移植，指利用干细胞能够在体内自我更新和多向分化的能力在病态免疫和造血功能被摧毁的机体内重建正常的造血和免疫功能。

Hal Broxmeyer, chairman and Mary Margaret Walther Professor of Microbiology/Immunology and scientific director of the Walther Oncology Center at Indiana University School of Medicine, said the new study "provides crucial, important, and physiologically relevant information on homing/engrafting capability … Most importantly, the results from this study open up the possibility of using a relatively simple means to enhance homing/engraftment and mobilization of" blood stem cells in bone marrow transplantation.

Mary Margaret Walther中心主席及微观生物学、免疫学教授、印第安纳大学医学院Walther 肿瘤中心科技主任Hal Broxmeyer称这一新的研究&为研究造血干细胞的移植/植活提供了关键、重要及生理学上相关的信息…最重要的是，这一研究成果开启了运用相对简便的方法增强&造血干细胞在骨髓移植上的&移植/植活及可移植性&。

The synergistic effect of IL-6 combined with G-CSF is better than that of either one. The mechanism of the effect is due to accelerati ve action on the recovery of residual bone marrow stem cells in irradiated mice. This procedure requires long time,and is not attributed to the inhibitory effect upon programmed cell death of bone marrow cells.

G-CSF和IL-6联合应用的效果优于单用的效果，其促进辐射后机体造血功能恢复的机理是通过刺激骨髓残余造血干细胞的恢复，该过程需要一段较长的时间，而不是通过抑制照后即刻骨髓细胞的程序性死亡。

During blood cell deelopment, the remoal of DNA adducts, the resealing of repair gaps, the resistance to DNA-reactie drugs clearly increased in stem or mature compared with progenitor cells of the same indiidual.

在血细胞发育过程中，造血干细胞和相应的成熟细胞对致DNA突变剂的去除，DNA缺口的填补，DNA反应药物的抵抗明显高于对应得造血祖细胞。

It is concluded that the expression of hERMAP in fetal liver approximately consistent with haematopoiesis of fetal liver, and the expression of this gene in bone marrow aged 15-32th fetal weeks coincides with haematopoiesis of fetal bone marrow.

人类ERMAP基因在胎儿肝脏中的表达规律与胎儿肝脏造血过程基本一致，在15-32周胎儿骨髓中的表达规律与胎儿期骨髓造血活动基本吻合，推测该基因的功能可能与胎儿发育过程中红系细胞向肝脏和骨髓的迁移活动有关。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。