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造血

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During the 100th anniversary of Red Cross founded in Shanghai,Sahnghai Red Cross Association set 2004 as "the year of humanity" to not only care about the orphans,sick children as well as the old in great need, but also accelerate the steps of database for blood-making in China to save more blood-affected sicks,adjust and manage the fund of hospital for infants in schools,put an eye on the health of children and propagandize the spirit of Red Cross.

为纪念中国红十字会在上海诞生百年,上海市红十字会把2004年定为&博爱年&,以&庆百年博爱行&为主题,开展关爱孤儿、病孩救助因病致贫的特困老人活动,对特困家庭进行人道救助,还将加快中国造血干细胞资料库建设,救助更多的血液病人,调整办好中小学婴幼儿少儿住院基金,为保护青少年幼儿健康服务,通过贯彻全年的&博爱行&把红十字关爱送进千家万户。

Objective: To investigate the optimal gene transfer protocols of hematopoietic cells mediated by retrovirus.

目的:摸索逆转录病毒介导的理想的造血细胞基因转染体系。

Blood or marrow stem cells are taken from a patient in remission. The cells are stored and then reinfused as soon as conditioning with chemotherapy and/or radiotherapy is completed.

自体造血干细胞回输是一种在缓解期提取患者自身的外周血或骨髓干细胞放在体外储存,当放化疗完成后再将细胞回输入患者体内的治疗方法

Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging.Cell,1991;

这些结果证实适当的细胞周期控制,特别在干/祖细胞早期阶段,是保持正常造血所必需的。

In 3 of 14 mice surviving long-term transplanted with bone marrow cells transduced with high-titer virus, bone marrow, spleen and thymus from two mice and bone marrow and spleen from another mouse contained the intact proviral genome. Long-term expression of the transferred gene was seen in one mouse at level of 7%of endogenous murine α-globin gene.

5%。在2只长期造血重建小鼠的骨髓、脾、胸腺及另一只小鼠的骨髓及脾中整合有人β-珠蛋白基因,在一只重建小鼠的骨髓和脾中有人β-珠蛋白基因表达,约为鼠α-珠蛋白基因的7 %。

The body weight and time for neutrophil and platelet recovery were measured in 20 children with acute leukemia.

本研究通过对脐血CD38 细胞亚群的检测,试图寻找其对脐血移植造血恢复的影响。

"Folic-acid-deficiency anemia: Anemia resulting from too little folic acid, needed for red-Blood-cell maturation. White-cell and platelet levels are also often low. Progressive gastrointestinal problems develop."

叶酸缺乏性贫血:因缺乏成熟红血球所必需的叶酸而引起贫血的一种疾病,常有白血球和血小板减少、骨髓造血障碍和进行性胃肠道疾状。

Keywords leukocyte reduction filter;transfusion;stem cell transplantation

去白细胞滤器;输血;造血干细胞移植

During the aplastic stage after hematopoietic stem cell transplantation, transfusion of platelet, anti-hemorrhage drugs and glucocorticoid were proved to be effectual methods to control the hemorrhagic complications of patients.

结论在造血干细胞移植后骨髓空虚期,及时采用输注新鲜单采血小板、应用止血药物及糖皮质激素等措施,能够有效控制患者出血并发症的发生。

At the third passage, BMSCs showed the typical polar swirl morphology. BMSCs were negative for CD34 and CD45, but positive for CD29 and CD44. Following induction, alkaline phosphatase staining, Oil red staining and toluidine blue staining produced a strong reaction in cells.

第3代骨髓间充质干细胞形态单一均匀,呈典型的极性漩涡状生长,不表达造血前体细胞标志抗原CD34和白细胞标志抗原CD45,表达整合素家族成员CD29和黏附分子CD44,经诱导后碱性磷酸酶染色、油红O染色和甲苯胺蓝染色均呈阳性。

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