通路

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Important family of negative feedback of JAK-STAT pathway: SOCS, PIAS, PTP is highly related with rheumatoid arthritis which will be the new ways for Clinical treatment, drug development and research of autoimmune diseases.

JAK-STAT通路负反馈调节的重要家族：SOCS、PIAS、PTP与类风湿关节炎高度相关，在未来可能成为自身免疫性疾病临床治疗、新药研发或科学研究的新途径。

PIAS proteins play roles mainly through two mechanisms: acting as SUMO E3 ligases to promote modifications of some transcriptional factors and cofactors, especially sumoylation, and to regulate their transcriptional activation; acting as structural proteins to offer platforms for protein-protein interactions and to promote the abstraction and recruitment of other regulators in the cellular signal pathway complexes or gene transcriptional complexes, companied by the subnuclear location of target proteins.

PIAS蛋白的调控机制主要有两种：一种是通过其自身所具有的SUMO(small ubiquitin-related modifiers)E3连接酶活性，促进对一些转录因子、转录辅因子的化学修饰，尤其是SUMO化修饰，从而调控它们的转录活性；另一种是作为构架蛋白，为蛋白质之间的相互作用提供平台，促进细胞信号通路复合物或基因转录复合物中其它调节蛋白的去除和募集，并涉及到靶蛋白的亚核定位。

Planning and managing pipeline inventory.

计划和管理通路库存。

In this review, the common pathway of apoptosis and the regulation in the placenta are described.

本文主要评述细胞凋亡的一般通路以及胎盘发生过程中细胞凋亡的调控。

The ends of the parison are at x=0 and x=2L,and there is symmetry about the plane x=L.

The末端原来在x = 0和x = 2L，并且关于第x飞机有对称性=通路

ABSTRACT Diabetic complications is related to the activation of polyalcohol pathway.

糖尿病并发症与糖代谢多元醇通路激活有关。

This articles reviews many hypothesis about pathogenesis of diabetic retinopathy,such as the increation of polyalcohol pathway,overdose of end product of glycosylated protein,activation of protein kinase C,increased production of active oxygen of mitochondria,factors of immune inflammation,heredity factors,any other factors and so on.

文章对多元醇通路的增加、糖基化终末产物的过量生成、蛋白激酶C的激活、线粒体活性氧产生增多、免疫炎症因素、遗传因素及其他因素等糖尿病视网膜病变发病机制的多种假说加以综述。

Genesis and development of DR is related to interaction among many etiological factors,including over expression of growth factor,hyperglycemia,hypertension,hemodynamic abnormality,end products of glycosylated protein,polyalcohol pathway,heredity and so on.

DR的发生和发展与生长因子过度表达、高血糖、高血压、血流动力学异常、糖基化蛋白终末产物、多元醇通路、遗传等诸多致病因素之间相互作用。

In addition, an experimental system using C language is established, including modules such as representation of waveform polynomial, decision of path senstization, delay computing, clocking based on single-period sensitization, clocking based on multi-period sensitization, test generation considering noise and transformation from bit-level waveform polynomial to word-level polynomial model. They respectively used to test models and techniques proposed in this paper.

另外，：基于C语言本人设计开发了一个实验软件系统，该系统包括波形多J一贞式表示模块、敏化通路判定模块、延时计算模块、单周期敏化的最小时钟周期精确确定模块、多周期敏化的最小时钟周期确定方法模块、考虑噪声的测试生成模块和位级波形多项式描述转化成字级多项式描述模块，分别用于对本文各章中提出的自动化设计的模型和方法进行实验验证。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。