通用

Chinaberry tree samples showing witches' broom were collected in Danzhou, Hainan Province. Using universal primers Rl6mF2/Rl6Mr1 for phytoplasma 16 S rDNA, a fragment of about 1.4 kb was amplified, cloned and sequenced from the DNA samples extracted from the diseased Chinaberry tree.

利用植原体16S rDNA通用引物对Rl6mF2/Rl6mR1，通过PCR技术从表现典型丛枝症状的苦楝植株总DNA扩增到约1.4 kb的特异片段，将此片段克隆后进行序列测定、分析及系统关系树构建。

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作；采用植原体16SrRNA基因通用引物（R16mF2/R16mR1及R16F2/R16R2）和/或植原体核糖体蛋白基因引物（rpF1/R1）对所采集到的病害标样总DNA进行PCR扩增，根据植原体特异扩增条带的出现，共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害，初步明确了云南省植原体病害的种类；通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列（已在GenBank中共登录近20余个相关序列），通过序列比较分析，明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性；对一些发生在草本植物上的植原体进行了株系保存；在国内核心期刊及一般期刊发表研究论文 9 篇，申请技术发明专利1项；初步建立了有关植原体病害的网页；培养研究生4名。

Keywords: Chinese character terminal,graphical kernel system,general purpose simulation program.

全文扼要讨论了这种软件的三个方面：（1）面向汉字终端的三码中西文系统的特点及新见解；（2）面向汉字、图形终端的图形核心系统GKS的设计；（3）能仿真多种终端的"通用仿真程序"的设计方法。

Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans. Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common Pathogenic non-Candida albicans (Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis), and the products were digested with the two restriction endonucleases (Msp Ⅰ and Hae Ⅲ) respectively. Results The four isolates consisted of 15 strains of Candida glabrata (7.50%), 7 strains of Candida parapsilosis (3.50%), 5 strains of Candida krusei (2.50%), 2 strains of Candida tropicalis (1.00%).

首先采用芽管试验、厚壁孢子试验、法国科玛嘉念珠菌显色培养基及API20CAUX酵母菌鉴定系统将分离自VVC患者阴道内的念珠菌菌株鉴定到种，然后采用真菌通用引物将4种常见非白念珠菌（包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌）进行PCR扩增，并选用MspⅠ和HaeⅢ两种内切酶对扩增产物进行酶切分析。

In recent years, as automobiles, machinery, aviation, aerospace and chemical industry have developed in the direction towards high performance, low power consumption and long life, general rubber such as nature rubber, chloroprene rubber, and acrylonitrile butadiene rubber etc can not satisfy thermal stability and oil resistance in new rubber products.

近年来，随着汽车、机械、航空、航天、化工等领域使用的橡胶制品向高性能、低能耗和长寿命方向发展，天然橡胶、氯丁橡胶、丁腈橡胶等通用橡胶已无法满足新型橡胶配件耐高温和耐油等方面的要求。

The most likely of GM's "safe" brands to face the chopping block is unquestionably Pontiac, however.

最有可能的通用汽车公司的"安全"品牌面对砧板无疑是庞蒂亚克，但是。

Now it appears as though GM's often forgotten subsidiary, Holden, may be next on the chopping block.

现在看来，好像通用常常被遗忘的附属，霍顿，可接下来砧板。

Chrysler Corporation used to be one of the three largest American automobile manufacturers whose brands include both passenger and commercial vehicles such as Chrysler, Jeep AE and Dodge.

克莱斯勒汽车公司：创建于1924年，曾是美国三大汽车制造商之一，另外两大汽车公司为通用和福特汽车公司。

Ford and GM dropped by more than 20% in 2008, and Chrysler by 30%.

福特汽车公司和通用公司在2008年的销量下降了20%，克莱斯勒公司则下降了30%。

We are pleased to see that GM and Chrysler have gotten out of bankruptcy.

我们很高兴的看到通用和克莱斯勒已经走出了破产的阴影。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。