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Objective To know the feasibility of detection of Bartonella sp.by automatic bacterial identification system.

目的了解全自动细菌鉴定系统检测巴尔通体的可行性。

The Bartonella in Yunnan not only shows phylogenetic diversity , but also shows specificity and there may be a potential species.

云南巴尔通体菌不仅具系统发育多样性,而且还具有其特异性和可能有潜在的新种。

Bartonella infection was found among small mammals in Hainan province, indicating risks of infection among human beings.

海南省小型兽类中有巴尔通体感染,存在人群感染的风险。

objective to investigate the phylogenic diversity of bartonella in yunnan province,p.r.china, by using phylogenetic analyses based on glta gene sequence comparisons.

目的 基于glta基因序列的系统发育分析对云南巴尔通体的多样性进行分析。

Bartonella sp.; Biochemical characteristics ; Automatic bacterial identification system

巴尔通体;生化特性;全自动细菌鉴定系统

"A new species called Bartonella rochalimae was recently discovered in a patient with an enlarged spleen who had travelled to South America," said Professor Chao-Chin Chang from the National Chung Hsing University in Taiwan.

& 最近在一个曾到过南美旅行的患有脾大的病人身上发现一种新的巴尔通体属细菌罗卡利马体菌,&来自台湾国立中兴大学的Chao-Chin Chang教授说。

Methods: To clarify the biochemical characteristics of Bartonella reference strains and isolates from China with ANT card in BioMerieux VITEK 32 microorganism analysis system and Rapid TD32 A system.

采用BioMerieux VITEK 32微生物分析系统的ANI厌氧菌鉴定卡及Rapid ID32A系统检测巴尔通体的各项生化指标。

China, by using phylogenetic analyses based on gltA gene sequence comparisons. Methods The 32 variants of Bartonella isolated in Yunnan Province and published in GenBank from 1999 to today were taken as the target strains.

用云南省1999年至今分离到的并已在GenBank中公布的32个巴尔通体变异体为目标株,利用网上核酸序列比对程序BLAST进行核酸序列同源性搜索,调出相似性最高且有效发表种典型菌株为对比株,进行比对和系统发育分析。

Methods the 32 variants of bartonella isolated in yunnan province and published in genbank from 1999 to today were taken as the target strains. the most closely related typical strains searched by blast in genbank with sequencing program were taken as comparative stains and their glta gene sequences were compared each other.

用云南省1999年至今分离到的并已在genbank中公布的32个巴尔通体变异体为目标株,利用网上核酸序列比对程序blast进行核酸序列同源性搜索,调出相似性最高且有效发表种典型菌株为对比株,进行比对和系统发育分析。

Results A total of 6 suspected colonies were isolated from 65 samples, all being Gram?negative small bacilli, and red for Gimanez staining. The PCR results confirmed that the six strains were all Bartonella , two isolated from Rattus losea and Rattus brunneusculue each, and one from Niniventer fulvescens and Suncus murinus each.

结果 从65 份样本中分离培养出疑似菌落6株,革兰染色镜检均见阴性小杆菌,Gimanez 染色为红色杆菌,经PCR 证实6 株均为巴尔通体,其中分离自黄毛鼠和屋顶鼠各2株,针毛鼠和臭鼩鼱各1株。

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