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逐渐融合

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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在囊胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Since the decontrol of economics and trade relationship between mainland and Taiwan from 1979, the shipping relationship has also made certain mitigation and development, which along with the gradual promotion of container transportation.

自1979年两岸经贸关系解冻开始,两岸的航运关系也有了一定的缓和与发展,集装箱的贸易和运输量也随着两岸关系的逐渐融合而日渐提升。

During this period, a great deal of Sogdian got into Tang Kingdom, and established several colonies along the Silk Road and in some inner commercial cities in China.

这些聚落成为粟特人商业活动的中转站和文化输入的桥头堡,同时,在民族融合的过程中,这些聚落依其各自的文化特点,以其不同的方式逐渐融合于汉族社会之中。

From then on, the Western Xia Dyna sty had conflicts and peace alternatively with the Eastern Yi.

西夏和东夷在长期的接触中逐渐融合,形成了华夏集团的前身。

The higher people's living standard is,the more obvious the spiritual and material of unity needs,we request that the spirit of the art of the introduction of specific material design, and design requirements of the entity to become the carrier of the arts,and then presents in front of the people compositely. This requires the art and design must be to a considerable extent to convergence.

人们的生活水平越高,对于精神与物质相统一的需求就越明显,既要求将艺术的精神引入具体的物质设计,又要求设计的实体成为艺术的载体,并复合地呈现在人们面前,这就要求艺术与设计必须在相当程度上走向趋同逐渐融合

A novel polymer of Poly(diglycidyl maleate-co-stearyl methacrylate) was synthesized and its structure was characterized by ~1H NMR. Its self-assembly behavior was studied in different solvents. It was found that vesicles and tubule-liked aggregates can be obtained through self-assembly of P(DGMA-co-SMA)/THF/EtOH system. And spheral aggregates were obtained in P(DGMA-co-SMA)/THF/H_2O system while the spheral aggregates were converted into rod-liked aggregates and line-liked aggregates after aged for different time.

合成了带含环氧基侧链和十八烷基侧链的聚(马来酸二缩水甘油酯-co-甲基丙烯酸十八酯),用~1H NMR对所得的共聚物进行了表征;研究了其在选择性溶剂中的自组装行为,发现P(DGMA-co-SMA)/THF/EtOH体系可以通过自组装形成囊泡形自组装体和支化的管状自组装体,P(DGMA-co-SMA)/THF/H_2O体系最初自组装形成球形自组装体,随着陈化时间的延长,球形自组装体逐渐融合成长条形自组装体和线形自组装体;探讨了自组装过程的机理,建立了可能的自组装过程模型。

His opinion of art, thought that artist and work of art must be interacted and integrated.

其对艺术的看法,认为艺术家和艺术品是相互影响的,逐渐融合

The manner of the central square is gradually intergraded into the masterplan design; the landscape divided the extra spaces in a natural way, and well established the penetrating relationship between the square and the buildings.

中心广场的手法也逐渐融合到建筑地块的总平面设计中,折线和景观自然地划分建筑以外剩下的地块,已达到广场和建筑相互渗透的关系。

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The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0,0.4,0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗,测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

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