逐渐增加的

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

In the 20 yr forest, 304. 6gm〓 C and 11. 6gm〓 N are inputted to the soil. In the 80 yr forest, 502. 3gm〓 C and 17. 2gm〓 N are inputted to the soil. In the climax forest, 430.3gm〓 C and 11. 9gm〓 N are inputted to the soil. Along with forest succession, the C inputted to the soil B layer by fine root increases because of the deepening of root depth distribution.

白桦山杨幼林、白桦山杨成熟林与原始阔叶红松林中，细根每年向林地周转的碳量分别为304.6gm〓、502.3gm〓、430.3gm〓，氮量分别为11.6gm〓、17.2gm〓、11.9gm〓；其中，随着演替的进行，由于根系分布的加深，细根每年向土壤B层周转的碳量会逐渐增加。

With the development of modern agriculture, the popularization of new cultivars, the improvement of cultivation measures, as well as with the increase of the cropping index, crop yield is increasing.

随着现代农业的发展，作物良种和高产栽培技术措施的推广和普及，使农作物产量逐渐增加，随农作物收获带走的硼亦随之增多，造成植物缺硼现象时有发生。

As temperature increases, PO blocks appear to be stretched conformers and strong inter-chain interaction with the formation of a hydrophobic core in the micellar phase.

随着温度增加，PO链段表现为逐渐伸展的构象伴随着较强的链间相互作用，形成疏水的胶团核。

For business card printing and membership card making business has been a gradual increase in a number of health, environmental protection, labour protection requirements and constraints, particularly on food packaging business card printing and membership card making more stringent requirements, so water is consistent with the international health standards.

对于制卡和会员卡制作行业来说，已逐渐增加了许多卫生、环保、劳动保护等方面的要求和制约条件，特别是对食品包装制卡和会员卡制作的要求更为严格，因此水性上光油也符合国际卫生标准的要求。

In the packaging business card printing and membership card production and commercial business card printing and membership card production, the gradual increase in recent years many health, environmental protection, labour protection requirements and constraints, particularly on food packaging business card printing and membership card making more stringent requirements to comply with international health standards.

在包装制卡和会员卡制作和商业制卡和会员卡制作等方面，近年来已逐渐增加了许多卫生、环保、劳动保护等方面的要求和制约条件，特别是对食品包装制卡和会员卡制作的要求更为严格，只有这样才能符合国际卫生标准的要求。

In the world, the sulfide content in petroleum is increasing with each passing day.

世界上供应的石油资源硫含量在逐渐增加，同时由于环境问题的突出，对汽油、柴油等石油产品硫含量制定了更加严格的标准。

The research employed nylon bag implantation and in vitro fermentation techniques. With the procession of in vitro fermentation, anaerobic fungal population density in the cultures were shown to increase, with the largest fungal populations growing on ryegrass leaves, ryegrass stem and straw occurring after 48 h of incubation.

结果表明，随体外发酵的进行，真菌数量逐渐增加，以黑麦草叶、黑麦草茎、稻草秸为底物时，瘤胃真菌数量均在48 h达到最大值，且真菌数量的最大值随底物木质素含量的上升呈下降趋势。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。