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To make it clear, using multilaser flow cytometry, molecular biologic technology and laser confocal microscope scan technology, we fixed eyes on mitochondrial permeability transition pores, mitochondrial transmembrane potential and caspases-3 (cysteinyl aspartate-specific proteinases) which are playing key roles of mitochondria. Meanwhile, the research also discussed the characteristics of Δψm of cells in cut specimenfrom esophageal carcinoma, adjacent tissue of esophageal carcinoma and the normal esophageal tissue.

为探讨食管癌细胞凋亡过程中线粒体所起的具体作用,本课题采用流式细胞检测、分子生物学和激光共聚焦扫描技术等手段,研究了食管癌细胞凋亡中,线粒体所起关键作用的环节—线粒体通透性转变孔(Mitochondrion Permeability Transition Pore,MPTP)、线粒体跨膜电位ΔΨm和线粒体释放反应中的Caspase-3(Cysteine protease-3);同时研究了食管癌手术切除标本中癌灶、癌旁和切端三处组织的细胞线粒体跨膜电位的特性。

Because the cell membrane is lipoid, lipid-soluble drugs diffuse more rapidly than relatively lipid-insoluble drugs.

由于细胞膜为类脂质,脂溶性药物的扩散比相对的非脂溶性药物更快些,小分子的透膜速度也要比大分子快。

Because the cell membrane is lipoid, lipid-soluble drugs diffuse more rapidly than relatively lipid-i oluble drugs.

由于细胞膜为类脂质,脂溶性药物的扩散比相对的非脂溶性药物更快些,小分子的透膜速度也要比大分子快。

Low molecular weight substances permeability provide a certain time of cell activity,semipermeable membrane immunoisolation technology can be potential method for allograft rejection

由于低分子物质的通透性是细胞活性的关键,半透膜免疫隔离技术能够保证细胞一定时间内的活性,可以作为解决异体移植排斥反应的一种手段。

Treatment with exogenous Spermine could enhance the increase in enzyme activity and delay the increase in permeability of plasma membrane and MDA content.

外源Spm可以提高水分胁迫下SOD、CAT和POD活性的上升幅度,减少膜脂过氧化产物MDA的积累和叶片质膜相对透性的上升幅度。

Despite of some difference effected by acetosalicylic acid between this two kind of plants,we found that the enhancement of relative membrane penetration by heavy metal stress was reduced, the peroxidation of membrane lipids was alleviated and to some degree protected the membrane from injury by Cd~(2+),thus ultimately promoted the tolerance ability of plants to heavy metal stress .

尽管乙酰水杨酸对两种实验材料发挥作用的浓度范围有一定差别,但在受镉离子污染的培养液中施加乙酰水杨酸后,乙酰水杨酸均能有效的抑制重金属Cd~(2+)胁迫引起的细胞膜透性增加、膜脂过氧化水平升高,从而延缓了重金属对膜结构的损伤,最终增强了植物体对水体重金属污染的抵抗能力。

Besides, the treatment could keep fresh weight, maintain stability of soluble sugar and anthocyanidin content and relative membrane permeability in petals tissue.

此外,该处理还具有保持花枝鲜重、维持花瓣中可溶性糖含量、花青素含量和花瓣细胞质膜相对透性的稳定等生理效应。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明:(1)在膜透性的研究中,丛毛羊胡子草抗寒性最强,采用电导率法测得半致死温度LT_(50最低,丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃;在可溶性蛋白含量研究中,抗寒锻炼前后三种植物的可溶性蛋白含量均增加,同时采用SDS-PAGE法,通过对蛋白条带分析,以丛毛羊胡子草条带数最多且着色深;(2)在可溶性糖的研究中,三种植物经抗寒锻炼后叶片可溶性糖含量都比抗寒锻炼前高,且经过抗寒锻炼后三种植物可溶性糖均呈下降趋势,丛毛羊胡子草可溶性糖含量下降较小;从游离脯氨酸含量可知,低温的胁迫影响了植物叶片游离脯氨酸的含量变化,植物的抗寒性与游离脯氨酸有关;低温胁迫下三种植物叶绿素含量随着温度的下降,叶绿素含量、叶绿素a、叶绿素b和叶绿素a/b均呈下降趋势,狗牙根对低温胁迫最为敏感;(3)低温锻炼前后,丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的,特别是过氧化物酶,说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

Effects of salt stress on protective enzyme system,MDA content and membrane permeability of NHC foliar

盐胁迫对NHC牧草叶片保护酶系统,MDA含量及膜透性的影响

A pot experiment was conducted to investigate the effect of soil water on protective enzymes of superoxide dismutase, catalase and peroxidase, and also on membrane lipid peroxidation including malonaldehyde and plasma membrane permeability in pea (Pisum sativum Linn.) leaf during flowering and pod-bearing stages under drought stress and water recovery conditions. Antioxidant ability of pea during flowering and pod-bearing stages was also analyzed.

为探讨不同水分条件对豌豆保护酶系统和膜脂过氧化的影响,采用盆栽人工控水试验方法,模拟干旱胁迫及复水条件,研究了不同水分处理对花荚期豌豆叶片超氧化物歧化酶、过氧化氢酶、过氧化物酶活性以及过氧化产物丙二醛含量和脂膜相对透性的影响,并对花荚期豌豆抗氧化能力进行了综合评价。

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