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The emodin did not inhibited multiplication of TMV in infected leaf discs or infectivity of TMV-RNA in vitro. The infectivity of TMV recovered by dialysis from incubations with emodin in vitro has regained showed that the emodin did not direct viricidal effect. The emodin did not reduce infectivity of TMV when applied before TMV inoculation.

大黄素对粒体无形态破坏作用,与TMV混合后经过透析可恢复大部分的侵染活性,体外混合不影响TMV-RNA的侵染活性,并无体内抑制复制作用,预先接种也不影响TMV随后的侵染。

The morphology of virions seems to not be change after incubated with the paeonol by electron microscope. The regained infectivity of TMV recovered by dialysis from incubation with paeonol in vitro showed that the paeonol have not direct viricidal effect. The paeonol did not reduce infectivity when applied before TMV inoculation. The paeonol in low concentration can restrain the polymerization of TMV coat protein in vitro. The paeonol did not reduced infectivity of TMV-RNA in vitro. It is proposed that the paeonol inhibited TMV by binding with TMV coat protein to interfere with recognition of a host receptor essential for infection or by interfering TMV-CP polymerization in plants. The anti-TMV activity of low molecular weight chemicals extracted from Polygonum cuspidatum were determined.

丹皮酚与TMV混合,电镜下观察对粒体形态没有影响;TMV与丹皮酚混和后透析可恢复侵染力,表明丹皮酚对病毒粒体并没有直接毒害作用;预先用丹皮酚喷施叶面不影响病毒的侵染,表明丹皮酚未干扰TMV对叶片侵染位点的识别;丹皮酚在低浓度时可阻碍TMV-CP的体外聚合,对TMV-RNA并无体外抑制侵染作用,据此推测丹皮酚对TMV的抑制作用可能主要通过其与TMV-CP结合影响病毒粒体对叶片表面侵染位点的识别,或影响体内TMV粒体的脱衣壳或TMV-CP亚基的体内聚合。

He results show that the virus regained infectivity, and the tannins did not direct viricidal effect. Tannins extracted from Polygonum perfoliatum or Archontophoenix alexandrae seeds showed strongly anti-TMV activities separately. TMV virions seems to be aggregated when TMV incubated with the tannins by electron microscope. It seems that TMV coat protein bind to the tannins based on PAGE. Tannins did not reduce infectivity when applied either before or after TMV inoculation. It is proposed that tannins extracted from the 11 species inhibited TMV by binding with TMV coat protein to interfere with recognition site essential for viral coat protein. Tannins extracted from Euphorbia hirta, P. perfoliatum, P. cuspidatum reduce infectivity when applied immediately in N.

MV分别与这11种植物单宁混合后,通过透析除去单宁后,可恢复大部分侵染力,表明单宁对病毒粒体并无直接毒害作用;TMV与杠板归和假槟榔种子中提取的单宁分别混合后,电镜下观察粒体呈现明显的聚集现象;TMV外壳蛋白分别与这两种植物单宁混合后,聚丙烯酰胺凝胶电泳中CP泳带滞后;单宁在病毒接种前施用未降低TMV对叶片的侵染能力;这些结果表明植物单宁对TMV抑制作用可能是单宁与TMV-CP结合影响病毒对叶片侵染位点的识别而降低TMV侵染性,而这种结合是可逆的。

This objective and neutral standing position, from the viewpoint of many more areas of Hebei, Baoding housing security system development and the existing problems in order to find ways to solve these problems the best way.

本文站在客观中立的立场,从多方面多视角透析河北保定地区住房保障制度的发展和存在的诸多问题,以期能找到方法来解决这些问题的最佳方法。

This objective and neutral standing position, from the viewpoint of many more areas of Hebei, Baoding housing security system development and the existing problems in order to find ways to solve these problems the best way.

本文站在客观中立的立场,从多方面多视角透析河北保定地区住房保障制度的发展和存在的诸多难题,以期能找到方法来处理这些问题的最佳方法。

This objective and neutral standing position, from the viewpoint of many more areas of Hebei, Baoding housing security system development and the existing problems in order to find ways to solve these problems the best way.

本文站在客观中立地立场,从多方面多视角透析河北保定地区住房保障制度的发展和存在的诸多难题,以期能找到方法来处理这些问题的最佳方法。

Methods: The releases of GABA and Gly from rat striatums during global ischemia were determined by microdialysis and HPLC methods.

用微透析和高压液相法测定大鼠半球缺血时纹体GABA和Gly的释放。

Methods: The releases of Glu, Asp, Gly, Tau and GABA from rat striatums during 30,60,90,120 min of global ischemia were determined by microdialysis and HPLC methods.

用微透析结合高效液相法测定半球缺血30,60,90,120分钟时大鼠纹体Glu,Asp,Gly,Tau和GABA的释放。

The binding of simazine to catalase in aqueous solution was studied using fluorescence spectroscopy and high performance liquid chromatography with microdialysis sampling technique.

应用荧光光谱法和微透析液相色谱法研究了水溶液中除草剂西玛津与过氧化氢酶分子间的相互作用。

There are generally two main drawbacks in the currently reported methods of dissolving wool, one of which is the low efficiency of dissolving fibers; only the wool keratin solutions with low concentration, low viscosity, relatively low molecular weight and low conversion rate have been obtained; the other drawback is, as mentioned in many patents, the wool keratin solution needed to be concentrated, purified, and coagulated into wool keratin powder, through back-permeation, dialysis techniques, in order to facilitate the application in the subsequent processes.

目前研究报道的羊毛纤维溶解方法都普遍存在有两个主要缺陷:其一是对纤维的溶解效率低,只能获得低浓度、低粘度、较低分子量、低制成率的羊毛角蛋白质溶液;其二则是因浓度低而在多数专利中需要通过反渗透、透析方法使溶液浓缩、提纯、固化成羊毛角蛋白质粉末,以方便后续加工使用。

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